Pseudorabies virus glycoproteins gII and gp50 are essential for virus penetration
Pseudorabies virus (PrV) glycoproteins gII and gp50 are major constituents of the viral envelope and targets of neutralizing monoclonal antibodies. Both are homologs of essential glycoproteins found in herpes simplex virus, gB (gII) and gD (gp50). We recently isolated a gII-negative PrV deletion mut...
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| Veröffentlicht in: | Journal of Virology 1991-10, Vol.65 (10), p.5348-5356 |
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| creator | Rauh, I. (Federal Research Centre for Virus Diseases of Animals, Tubingen, Germany) Mettenleiter, T.C |
| description | Pseudorabies virus (PrV) glycoproteins gII and gp50 are major constituents of the viral envelope and targets of neutralizing monoclonal antibodies. Both are homologs of essential glycoproteins found in herpes simplex virus, gB (gII) and gD (gp50). We recently isolated a gII-negative PrV deletion mutant on complementing cell lines and established the essential character of gII for PrV replication (I. Rauh, F. Weiland, F. Fehler, G. Keil, and T. C. Mettenleiter, J. Virol. 65:621-631, 1991). In this report, we describe the isolation of a gp50-negative PrV mutant after constructing cell lines that constitutively express gp50 and phenotypically complement the gp50 defect. Analysis of the gp50- mutant proved that gp50 is essential for PrV replication. Further studies showed that both gII and gp50 are required for viral penetration into target cells. The penetration defect in the gII and gp50 deletion mutants could be overcome by experimental polyethylene glycol-induced membrane fusion. Surprisingly, whereas gII proved to be essential for both penetration and cell-cell spread of the virus, gp50 was required only for penetration and appeared dispensable for direct cell-cell spread |
| doi_str_mv | 10.1128/jvi.65.10.5348-5356.1991 |
| format | Article |
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(Federal Research Centre for Virus Diseases of Animals, Tubingen, Germany) ; Mettenleiter, T.C</creator><creatorcontrib>Rauh, I. (Federal Research Centre for Virus Diseases of Animals, Tubingen, Germany) ; Mettenleiter, T.C</creatorcontrib><description>Pseudorabies virus (PrV) glycoproteins gII and gp50 are major constituents of the viral envelope and targets of neutralizing monoclonal antibodies. Both are homologs of essential glycoproteins found in herpes simplex virus, gB (gII) and gD (gp50). We recently isolated a gII-negative PrV deletion mutant on complementing cell lines and established the essential character of gII for PrV replication (I. Rauh, F. Weiland, F. Fehler, G. Keil, and T. C. Mettenleiter, J. Virol. 65:621-631, 1991). In this report, we describe the isolation of a gp50-negative PrV mutant after constructing cell lines that constitutively express gp50 and phenotypically complement the gp50 defect. Analysis of the gp50- mutant proved that gp50 is essential for PrV replication. Further studies showed that both gII and gp50 are required for viral penetration into target cells. The penetration defect in the gII and gp50 deletion mutants could be overcome by experimental polyethylene glycol-induced membrane fusion. Surprisingly, whereas gII proved to be essential for both penetration and cell-cell spread of the virus, gp50 was required only for penetration and appeared dispensable for direct cell-cell spread</description><identifier>ISSN: 0022-538X</identifier><identifier>EISSN: 1098-5514</identifier><identifier>DOI: 10.1128/jvi.65.10.5348-5356.1991</identifier><identifier>PMID: 1654444</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Animals ; Biological and medical sciences ; Blotting, Southern ; Cell Line ; CELLULE ; CELULAS ; DNA, Viral - genetics ; DNA, Viral - isolation & purification ; Fundamental and applied biological sciences. Psychology ; Genes, Viral ; GLICOPROTEINAS ; GLYCOPROTEINE ; Herpesvirus 1, Suid - genetics ; Herpesvirus 1, Suid - physiology ; HERPETOVIRIDAE ; Microbiology ; Mutagenesis ; MUTANT ; MUTANTES ; Plasmids ; Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains ; Restriction Mapping ; Viral Envelope Proteins - genetics ; Viral Envelope Proteins - metabolism ; Viral Proteins - biosynthesis ; Viral Proteins - genetics ; Virion - genetics ; Virion - physiology ; Virology ; Virus Replication</subject><ispartof>Journal of Virology, 1991-10, Vol.65 (10), p.5348-5356</ispartof><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c586t-2d4a0d84de5a691442bb59afdd48f86d270e513e43375c2f5bbbaf3125dfdfc63</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC249015/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC249015/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27915,27916,53782,53784</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5160517$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1654444$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rauh, I. (Federal Research Centre for Virus Diseases of Animals, Tubingen, Germany)</creatorcontrib><creatorcontrib>Mettenleiter, T.C</creatorcontrib><title>Pseudorabies virus glycoproteins gII and gp50 are essential for virus penetration</title><title>Journal of Virology</title><addtitle>J Virol</addtitle><description>Pseudorabies virus (PrV) glycoproteins gII and gp50 are major constituents of the viral envelope and targets of neutralizing monoclonal antibodies. Both are homologs of essential glycoproteins found in herpes simplex virus, gB (gII) and gD (gp50). We recently isolated a gII-negative PrV deletion mutant on complementing cell lines and established the essential character of gII for PrV replication (I. Rauh, F. Weiland, F. Fehler, G. Keil, and T. C. Mettenleiter, J. Virol. 65:621-631, 1991). In this report, we describe the isolation of a gp50-negative PrV mutant after constructing cell lines that constitutively express gp50 and phenotypically complement the gp50 defect. Analysis of the gp50- mutant proved that gp50 is essential for PrV replication. Further studies showed that both gII and gp50 are required for viral penetration into target cells. The penetration defect in the gII and gp50 deletion mutants could be overcome by experimental polyethylene glycol-induced membrane fusion. Surprisingly, whereas gII proved to be essential for both penetration and cell-cell spread of the virus, gp50 was required only for penetration and appeared dispensable for direct cell-cell spread</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blotting, Southern</subject><subject>Cell Line</subject><subject>CELLULE</subject><subject>CELULAS</subject><subject>DNA, Viral - genetics</subject><subject>DNA, Viral - isolation & purification</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Viral</subject><subject>GLICOPROTEINAS</subject><subject>GLYCOPROTEINE</subject><subject>Herpesvirus 1, Suid - genetics</subject><subject>Herpesvirus 1, Suid - physiology</subject><subject>HERPETOVIRIDAE</subject><subject>Microbiology</subject><subject>Mutagenesis</subject><subject>MUTANT</subject><subject>MUTANTES</subject><subject>Plasmids</subject><subject>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</subject><subject>Restriction Mapping</subject><subject>Viral Envelope Proteins - genetics</subject><subject>Viral Envelope Proteins - metabolism</subject><subject>Viral Proteins - biosynthesis</subject><subject>Viral Proteins - genetics</subject><subject>Virion - genetics</subject><subject>Virion - physiology</subject><subject>Virology</subject><subject>Virus Replication</subject><issn>0022-538X</issn><issn>1098-5514</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkl2L1DAUhoMo67j6BwShiHg3Y5ImaXrhhSx-DCyo6IJ34bQ56WTpNDVpR_bfm9Jh_chNOHmf9-SQN4QUjO4Y4_rN7cnvlNzlUpZCb2Up1Y7VNXtANozW-UAy8ZBsKOU8i_rHY_IkpVtKmRBKXJALpqTIa0O-fkk42xCh8ZiKk49zKrr-rg1jDBP6IVf7fQGDLbpR0gIiFpgSDpOHvnAhni0jDjhFmHwYnpJHDvqEz877Jbn58P771aft9eeP-6t319tWajVtuRVArRYWJag6z8WbRtbgrBXaaWV5RVGyEkVZVrLlTjZNA65kXFpnXavKS_J27TvOzRFtm2eK0Jsx-iPEOxPAm3-VwR9MF06Gi5oymf2vz_4Yfs6YJnP0qcW-hwHDnAxTnGpd8QzqFWxjSCmiu7-DUbOkYXIaRsmlXNIwSxpmSSNbX_w94x_j-vxZf3XWIbXQuwhD69M9JpmiklUZe7liB98dfvmIBtLxv1sz9HyFHAQDXcx9br7VrGL5E5S_AdNYqeI</recordid><startdate>19911001</startdate><enddate>19911001</enddate><creator>Rauh, I. (Federal Research Centre for Virus Diseases of Animals, Tubingen, Germany)</creator><creator>Mettenleiter, T.C</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>5PM</scope></search><sort><creationdate>19911001</creationdate><title>Pseudorabies virus glycoproteins gII and gp50 are essential for virus penetration</title><author>Rauh, I. (Federal Research Centre for Virus Diseases of Animals, Tubingen, Germany) ; Mettenleiter, T.C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c586t-2d4a0d84de5a691442bb59afdd48f86d270e513e43375c2f5bbbaf3125dfdfc63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blotting, Southern</topic><topic>Cell Line</topic><topic>CELLULE</topic><topic>CELULAS</topic><topic>DNA, Viral - genetics</topic><topic>DNA, Viral - isolation & purification</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Viral</topic><topic>GLICOPROTEINAS</topic><topic>GLYCOPROTEINE</topic><topic>Herpesvirus 1, Suid - genetics</topic><topic>Herpesvirus 1, Suid - physiology</topic><topic>HERPETOVIRIDAE</topic><topic>Microbiology</topic><topic>Mutagenesis</topic><topic>MUTANT</topic><topic>MUTANTES</topic><topic>Plasmids</topic><topic>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</topic><topic>Restriction Mapping</topic><topic>Viral Envelope Proteins - genetics</topic><topic>Viral Envelope Proteins - metabolism</topic><topic>Viral Proteins - biosynthesis</topic><topic>Viral Proteins - genetics</topic><topic>Virion - genetics</topic><topic>Virion - physiology</topic><topic>Virology</topic><topic>Virus Replication</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rauh, I. (Federal Research Centre for Virus Diseases of Animals, Tubingen, Germany)</creatorcontrib><creatorcontrib>Mettenleiter, T.C</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rauh, I. (Federal Research Centre for Virus Diseases of Animals, Tubingen, Germany)</au><au>Mettenleiter, T.C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pseudorabies virus glycoproteins gII and gp50 are essential for virus penetration</atitle><jtitle>Journal of Virology</jtitle><addtitle>J Virol</addtitle><date>1991-10-01</date><risdate>1991</risdate><volume>65</volume><issue>10</issue><spage>5348</spage><epage>5356</epage><pages>5348-5356</pages><issn>0022-538X</issn><eissn>1098-5514</eissn><abstract>Pseudorabies virus (PrV) glycoproteins gII and gp50 are major constituents of the viral envelope and targets of neutralizing monoclonal antibodies. Both are homologs of essential glycoproteins found in herpes simplex virus, gB (gII) and gD (gp50). We recently isolated a gII-negative PrV deletion mutant on complementing cell lines and established the essential character of gII for PrV replication (I. Rauh, F. Weiland, F. Fehler, G. Keil, and T. C. Mettenleiter, J. Virol. 65:621-631, 1991). In this report, we describe the isolation of a gp50-negative PrV mutant after constructing cell lines that constitutively express gp50 and phenotypically complement the gp50 defect. Analysis of the gp50- mutant proved that gp50 is essential for PrV replication. Further studies showed that both gII and gp50 are required for viral penetration into target cells. The penetration defect in the gII and gp50 deletion mutants could be overcome by experimental polyethylene glycol-induced membrane fusion. Surprisingly, whereas gII proved to be essential for both penetration and cell-cell spread of the virus, gp50 was required only for penetration and appeared dispensable for direct cell-cell spread</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>1654444</pmid><doi>10.1128/jvi.65.10.5348-5356.1991</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Biological and medical sciences Blotting, Southern Cell Line CELLULE CELULAS DNA, Viral - genetics DNA, Viral - isolation & purification Fundamental and applied biological sciences. Psychology Genes, Viral GLICOPROTEINAS GLYCOPROTEINE Herpesvirus 1, Suid - genetics Herpesvirus 1, Suid - physiology HERPETOVIRIDAE Microbiology Mutagenesis MUTANT MUTANTES Plasmids Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains Restriction Mapping Viral Envelope Proteins - genetics Viral Envelope Proteins - metabolism Viral Proteins - biosynthesis Viral Proteins - genetics Virion - genetics Virion - physiology Virology Virus Replication |
| title | Pseudorabies virus glycoproteins gII and gp50 are essential for virus penetration |
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