Cardiovascular responses to electrical stimulation of sympathetic nerves in the pithed mouse
Abstract The pithed rat model has been used extensively to study peripheral cardiovascular responses to electrical stimulation of the sympathetic nervous system, as pithing eliminates central and reflex effects. However, since the transgenic mouse has become a standard and economical model organism,...
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| Veröffentlicht in: | Autonomic neuroscience 2008-06, Vol.140 (1), p.49-52 |
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| creator | Elayan, Hamzeh H Sun, Ping Milic, Milos Liu, Fujun Bao, Xuping Ziegler, Michael G |
| description | Abstract The pithed rat model has been used extensively to study peripheral cardiovascular responses to electrical stimulation of the sympathetic nervous system, as pithing eliminates central and reflex effects. However, since the transgenic mouse has become a standard and economical model organism, an electrically stimulated pithed mouse would facilitate a variety of studies. We have developed surgical techniques, drug doses and stimulation parameters for an electrically stimulated pithed mouse to study peripheral sympathetic nerve effects on blood pressure. Similar to the pithed rat, the pithed mouse showed voltage and frequency-dependent blood pressure responses to a pulsed train of electrical stimuli. In addition, α-adrenergic stimulation with phenylephrine gave a marked systolic pressor response, while the β2 agonist salbutamol lowered diastolic blood pressure. Furthermore, pithed transgenic mice unable to synthesize catecholamines in adrenergic cells displayed smaller pressor responses than pithed control mice. In summary, the electrically stimulated pithed mouse can be used to study peripheral effects of the sympathetic system on cardiovascular dynamics unencumbered by central responses. |
| doi_str_mv | 10.1016/j.autneu.2008.03.003 |
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However, since the transgenic mouse has become a standard and economical model organism, an electrically stimulated pithed mouse would facilitate a variety of studies. We have developed surgical techniques, drug doses and stimulation parameters for an electrically stimulated pithed mouse to study peripheral sympathetic nerve effects on blood pressure. Similar to the pithed rat, the pithed mouse showed voltage and frequency-dependent blood pressure responses to a pulsed train of electrical stimuli. In addition, α-adrenergic stimulation with phenylephrine gave a marked systolic pressor response, while the β2 agonist salbutamol lowered diastolic blood pressure. Furthermore, pithed transgenic mice unable to synthesize catecholamines in adrenergic cells displayed smaller pressor responses than pithed control mice. In summary, the electrically stimulated pithed mouse can be used to study peripheral effects of the sympathetic system on cardiovascular dynamics unencumbered by central responses.</description><identifier>ISSN: 1566-0702</identifier><identifier>EISSN: 1872-7484</identifier><identifier>DOI: 10.1016/j.autneu.2008.03.003</identifier><identifier>PMID: 18407806</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Adrenergic alpha-Agonists - pharmacology ; Adrenergic beta-Agonists - pharmacology ; Adrenergic Fibers - drug effects ; Adrenergic Fibers - metabolism ; Adrenergic Fibers - secretion ; Advanced Basic Science ; Animals ; Biological and medical sciences ; Blood pressure ; Blood Pressure - drug effects ; Blood Pressure - genetics ; Blood Pressure - physiology ; Catecholamine ; Catecholamines - genetics ; Catecholamines - metabolism ; Catecholamines - secretion ; Decerebrate State ; Denervation ; Diastole - drug effects ; Diastole - genetics ; Electric Stimulation ; Electrical stimulation ; Epinephrine ; Fundamental and applied biological sciences. Psychology ; Heart - drug effects ; Heart - innervation ; Heart - physiology ; Medical Education ; Membrane Potentials - drug effects ; Membrane Potentials - physiology ; Mice ; Mice, Inbred C57BL ; Mice, Transgenic ; Models, Animal ; Neurosurgical Procedures - methods ; Peripheral nervous system. Autonomic nervous system. Neuromuscular transmission. Ganglionic transmission. Electric organ ; Phenylethanolamine N-methyltransferase ; Pithed mouse ; Synaptic Transmission - drug effects ; Synaptic Transmission - genetics ; Systole - drug effects ; Systole - genetics ; Vertebrates: nervous system and sense organs</subject><ispartof>Autonomic neuroscience, 2008-06, Vol.140 (1), p.49-52</ispartof><rights>Elsevier B.V.</rights><rights>2008 Elsevier B.V.</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c578t-5079f7e1cd017e709c4fd6b37d5f00184b642261a5a8e86a5c3fee1e46a13bf43</citedby><cites>FETCH-LOGICAL-c578t-5079f7e1cd017e709c4fd6b37d5f00184b642261a5a8e86a5c3fee1e46a13bf43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.autneu.2008.03.003$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20435462$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18407806$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Elayan, Hamzeh H</creatorcontrib><creatorcontrib>Sun, Ping</creatorcontrib><creatorcontrib>Milic, Milos</creatorcontrib><creatorcontrib>Liu, Fujun</creatorcontrib><creatorcontrib>Bao, Xuping</creatorcontrib><creatorcontrib>Ziegler, Michael G</creatorcontrib><title>Cardiovascular responses to electrical stimulation of sympathetic nerves in the pithed mouse</title><title>Autonomic neuroscience</title><addtitle>Auton Neurosci</addtitle><description>Abstract The pithed rat model has been used extensively to study peripheral cardiovascular responses to electrical stimulation of the sympathetic nervous system, as pithing eliminates central and reflex effects. However, since the transgenic mouse has become a standard and economical model organism, an electrically stimulated pithed mouse would facilitate a variety of studies. We have developed surgical techniques, drug doses and stimulation parameters for an electrically stimulated pithed mouse to study peripheral sympathetic nerve effects on blood pressure. Similar to the pithed rat, the pithed mouse showed voltage and frequency-dependent blood pressure responses to a pulsed train of electrical stimuli. In addition, α-adrenergic stimulation with phenylephrine gave a marked systolic pressor response, while the β2 agonist salbutamol lowered diastolic blood pressure. Furthermore, pithed transgenic mice unable to synthesize catecholamines in adrenergic cells displayed smaller pressor responses than pithed control mice. In summary, the electrically stimulated pithed mouse can be used to study peripheral effects of the sympathetic system on cardiovascular dynamics unencumbered by central responses.</description><subject>Adrenergic alpha-Agonists - pharmacology</subject><subject>Adrenergic beta-Agonists - pharmacology</subject><subject>Adrenergic Fibers - drug effects</subject><subject>Adrenergic Fibers - metabolism</subject><subject>Adrenergic Fibers - secretion</subject><subject>Advanced Basic Science</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blood pressure</subject><subject>Blood Pressure - drug effects</subject><subject>Blood Pressure - genetics</subject><subject>Blood Pressure - physiology</subject><subject>Catecholamine</subject><subject>Catecholamines - genetics</subject><subject>Catecholamines - metabolism</subject><subject>Catecholamines - secretion</subject><subject>Decerebrate State</subject><subject>Denervation</subject><subject>Diastole - drug effects</subject><subject>Diastole - genetics</subject><subject>Electric Stimulation</subject><subject>Electrical stimulation</subject><subject>Epinephrine</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Heart - drug effects</subject><subject>Heart - innervation</subject><subject>Heart - physiology</subject><subject>Medical Education</subject><subject>Membrane Potentials - drug effects</subject><subject>Membrane Potentials - physiology</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Transgenic</subject><subject>Models, Animal</subject><subject>Neurosurgical Procedures - methods</subject><subject>Peripheral nervous system. Autonomic nervous system. Neuromuscular transmission. Ganglionic transmission. Electric organ</subject><subject>Phenylethanolamine N-methyltransferase</subject><subject>Pithed mouse</subject><subject>Synaptic Transmission - drug effects</subject><subject>Synaptic Transmission - genetics</subject><subject>Systole - drug effects</subject><subject>Systole - genetics</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>1566-0702</issn><issn>1872-7484</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkk-P1SAUxRujcf7oNzCmG3XVeikU6MZk8qKjySQu1J0J4dFbh2cLFehL3reX-l5m1IVugMCPk3vvOUXxjEBNgPDXu1ovyeFSNwCyBloD0AfFOZGiqQST7GE-t5xXIKA5Ky5i3EEGoeOPizMiGQgJ_Lz4utGht36vo1lGHcqAcfYuYiyTL3FEk4I1eixjslMGkvWu9EMZD9Os0y0ma0qHYZ9568p8Uc42r305-SXik-LRoMeIT0_7ZfHl3dvPm_fVzcfrD5urm8q0QqaqBdENAonpgQgU0Bk29HxLRd8OALnWLWdNw4lutUTJdWvogEiQcU3odmD0snhz1J2X7YS9QZeCHtUc7KTDQXlt1Z8vzt6qb36vGiY5FZAFXp0Egv-xYExqstHgOGqHuRElJQXatEAz-fKfpCCcMdKtkuwImuBjDDjclUNArQaqnToaqFYDFVAFv_Sf_97K_aeTYxl4cQKyZXocgnbGxjuuAUZbxpv7mWAe_N5iUNFYdAZ7G7Krqvf2f5X8LWBG69YwfMcDxp1fgsumKqJio0B9WsO2Zi1nLH9nHf0JAkXStg</recordid><startdate>20080601</startdate><enddate>20080601</enddate><creator>Elayan, Hamzeh H</creator><creator>Sun, Ping</creator><creator>Milic, Milos</creator><creator>Liu, Fujun</creator><creator>Bao, Xuping</creator><creator>Ziegler, Michael G</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TK</scope><scope>5PM</scope></search><sort><creationdate>20080601</creationdate><title>Cardiovascular responses to electrical stimulation of sympathetic nerves in the pithed mouse</title><author>Elayan, Hamzeh H ; Sun, Ping ; Milic, Milos ; Liu, Fujun ; Bao, Xuping ; Ziegler, Michael G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c578t-5079f7e1cd017e709c4fd6b37d5f00184b642261a5a8e86a5c3fee1e46a13bf43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Adrenergic alpha-Agonists - pharmacology</topic><topic>Adrenergic beta-Agonists - pharmacology</topic><topic>Adrenergic Fibers - drug effects</topic><topic>Adrenergic Fibers - metabolism</topic><topic>Adrenergic Fibers - secretion</topic><topic>Advanced Basic Science</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blood pressure</topic><topic>Blood Pressure - drug effects</topic><topic>Blood Pressure - genetics</topic><topic>Blood Pressure - physiology</topic><topic>Catecholamine</topic><topic>Catecholamines - genetics</topic><topic>Catecholamines - metabolism</topic><topic>Catecholamines - secretion</topic><topic>Decerebrate State</topic><topic>Denervation</topic><topic>Diastole - drug effects</topic><topic>Diastole - genetics</topic><topic>Electric Stimulation</topic><topic>Electrical stimulation</topic><topic>Epinephrine</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Heart - drug effects</topic><topic>Heart - innervation</topic><topic>Heart - physiology</topic><topic>Medical Education</topic><topic>Membrane Potentials - drug effects</topic><topic>Membrane Potentials - physiology</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Transgenic</topic><topic>Models, Animal</topic><topic>Neurosurgical Procedures - methods</topic><topic>Peripheral nervous system. Autonomic nervous system. Neuromuscular transmission. Ganglionic transmission. Electric organ</topic><topic>Phenylethanolamine N-methyltransferase</topic><topic>Pithed mouse</topic><topic>Synaptic Transmission - drug effects</topic><topic>Synaptic Transmission - genetics</topic><topic>Systole - drug effects</topic><topic>Systole - genetics</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Elayan, Hamzeh H</creatorcontrib><creatorcontrib>Sun, Ping</creatorcontrib><creatorcontrib>Milic, Milos</creatorcontrib><creatorcontrib>Liu, Fujun</creatorcontrib><creatorcontrib>Bao, Xuping</creatorcontrib><creatorcontrib>Ziegler, Michael G</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Neurosciences Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Autonomic neuroscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Elayan, Hamzeh H</au><au>Sun, Ping</au><au>Milic, Milos</au><au>Liu, Fujun</au><au>Bao, Xuping</au><au>Ziegler, Michael G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cardiovascular responses to electrical stimulation of sympathetic nerves in the pithed mouse</atitle><jtitle>Autonomic neuroscience</jtitle><addtitle>Auton Neurosci</addtitle><date>2008-06-01</date><risdate>2008</risdate><volume>140</volume><issue>1</issue><spage>49</spage><epage>52</epage><pages>49-52</pages><issn>1566-0702</issn><eissn>1872-7484</eissn><abstract>Abstract The pithed rat model has been used extensively to study peripheral cardiovascular responses to electrical stimulation of the sympathetic nervous system, as pithing eliminates central and reflex effects. However, since the transgenic mouse has become a standard and economical model organism, an electrically stimulated pithed mouse would facilitate a variety of studies. We have developed surgical techniques, drug doses and stimulation parameters for an electrically stimulated pithed mouse to study peripheral sympathetic nerve effects on blood pressure. Similar to the pithed rat, the pithed mouse showed voltage and frequency-dependent blood pressure responses to a pulsed train of electrical stimuli. In addition, α-adrenergic stimulation with phenylephrine gave a marked systolic pressor response, while the β2 agonist salbutamol lowered diastolic blood pressure. Furthermore, pithed transgenic mice unable to synthesize catecholamines in adrenergic cells displayed smaller pressor responses than pithed control mice. In summary, the electrically stimulated pithed mouse can be used to study peripheral effects of the sympathetic system on cardiovascular dynamics unencumbered by central responses.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>18407806</pmid><doi>10.1016/j.autneu.2008.03.003</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Autonomic neuroscience, 2008-06, Vol.140 (1), p.49-52 |
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| subjects | Adrenergic alpha-Agonists - pharmacology Adrenergic beta-Agonists - pharmacology Adrenergic Fibers - drug effects Adrenergic Fibers - metabolism Adrenergic Fibers - secretion Advanced Basic Science Animals Biological and medical sciences Blood pressure Blood Pressure - drug effects Blood Pressure - genetics Blood Pressure - physiology Catecholamine Catecholamines - genetics Catecholamines - metabolism Catecholamines - secretion Decerebrate State Denervation Diastole - drug effects Diastole - genetics Electric Stimulation Electrical stimulation Epinephrine Fundamental and applied biological sciences. Psychology Heart - drug effects Heart - innervation Heart - physiology Medical Education Membrane Potentials - drug effects Membrane Potentials - physiology Mice Mice, Inbred C57BL Mice, Transgenic Models, Animal Neurosurgical Procedures - methods Peripheral nervous system. Autonomic nervous system. Neuromuscular transmission. Ganglionic transmission. Electric organ Phenylethanolamine N-methyltransferase Pithed mouse Synaptic Transmission - drug effects Synaptic Transmission - genetics Systole - drug effects Systole - genetics Vertebrates: nervous system and sense organs |
| title | Cardiovascular responses to electrical stimulation of sympathetic nerves in the pithed mouse |
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