Muscle-like nicotinic receptor accessory molecules in sensory hair cells of the inner ear
Nothing is known about the regulation of nicotinic acetylcholine receptors (nAChRs) in hair cells of the inner ear. MuSK, rapsyn and RIC-3 are accessory molecules associated with muscle and brain nAChR function. We demonstrate that these accessory molecules are expressed in the inner ear raising the...
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| Veröffentlicht in: | Molecular and cellular neuroscience 2008-06, Vol.38 (2), p.153-169 |
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| creator | Osman, Abdullah A. Schrader, Angela D. Hawkes, Aubrey J. Akil, Omar Bergeron, Adam Lustig, Lawrence R. Simmons, Dwayne D. |
| description | Nothing is known about the regulation of nicotinic acetylcholine receptors (nAChRs) in hair cells of the inner ear. MuSK, rapsyn and RIC-3 are accessory molecules associated with muscle and brain nAChR function. We demonstrate that these accessory molecules are expressed in the inner ear raising the possibility of a muscle-like mechanism for clustering and assembly of nAChRs in hair cells. We focused our investigations on rapsyn and RIC-3. Rapsyn interacts with the cytoplasmic loop of nAChR α9 subunits but not nAChR α10 subunits. Although rapsyn and RIC-3 increase nAChR α9 expression, rapsyn plays a greater role in receptor clustering while RIC-3 is important for acetylcholine-induced calcium responses. Our data suggest that RIC-3 facilitates receptor function, while rapsyn enhances receptor clustering at the cell surface. |
| doi_str_mv | 10.1016/j.mcn.2008.02.008 |
| format | Article |
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MuSK, rapsyn and RIC-3 are accessory molecules associated with muscle and brain nAChR function. We demonstrate that these accessory molecules are expressed in the inner ear raising the possibility of a muscle-like mechanism for clustering and assembly of nAChRs in hair cells. We focused our investigations on rapsyn and RIC-3. Rapsyn interacts with the cytoplasmic loop of nAChR α9 subunits but not nAChR α10 subunits. Although rapsyn and RIC-3 increase nAChR α9 expression, rapsyn plays a greater role in receptor clustering while RIC-3 is important for acetylcholine-induced calcium responses. 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MuSK, rapsyn and RIC-3 are accessory molecules associated with muscle and brain nAChR function. We demonstrate that these accessory molecules are expressed in the inner ear raising the possibility of a muscle-like mechanism for clustering and assembly of nAChRs in hair cells. We focused our investigations on rapsyn and RIC-3. Rapsyn interacts with the cytoplasmic loop of nAChR α9 subunits but not nAChR α10 subunits. Although rapsyn and RIC-3 increase nAChR α9 expression, rapsyn plays a greater role in receptor clustering while RIC-3 is important for acetylcholine-induced calcium responses. Our data suggest that RIC-3 facilitates receptor function, while rapsyn enhances receptor clustering at the cell surface.</description><subject>Animals</subject><subject>Bungarotoxins - pharmacology</subject><subject>Calcium - metabolism</subject><subject>Cholinergic synapse</subject><subject>Female</subject><subject>Hair Cells, Auditory, Inner - cytology</subject><subject>Hair Cells, Auditory, Inner - metabolism</subject><subject>Humans</subject><subject>Intracellular Signaling Peptides and Proteins - genetics</subject><subject>Intracellular Signaling Peptides and Proteins - metabolism</subject><subject>Kidney - cytology</subject><subject>LLC-PK1 Cells</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Muscle Proteins - metabolism</subject><subject>Peripheral auditory system</subject><subject>Pregnancy</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Receptor assembly and clustering</subject><subject>Receptor Protein-Tyrosine Kinases - genetics</subject><subject>Receptor Protein-Tyrosine Kinases - metabolism</subject><subject>Receptors, Cholinergic - genetics</subject><subject>Receptors, Cholinergic - metabolism</subject><subject>Receptors, Nicotinic - genetics</subject><subject>Receptors, Nicotinic - metabolism</subject><subject>RNA, Messenger - metabolism</subject><subject>Swine</subject><subject>Synapses - metabolism</subject><subject>Synaptogenesis</subject><subject>Transfection</subject><issn>1044-7431</issn><issn>1095-9327</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUU2P1DAMrRCI_YAfwAXlxK3FTvqRCgkJrZYFaREXOHCKMq7LZGiTIWlX2n9PhhnxcYEc_Cz7-SXOK4pnCBUCti931Uy-kgC6AllleFCcI_RN2SvZPTzkdV12tcKz4iKlHQA0slePizPUtYQa-_Piy4c10cTl5L6x8I7C4nIUkYn3S4jCEnFKId6LOUxM68RJOC8S-5_FrXVREE9TEmEUy5Zz03MUbOOT4tFop8RPT3hZfH57_enqXXn78eb91ZvbkmoNS9lvOlU30G6alrqRUGrWCM1gh5HzkQiWQfeIhIqoYdQ50YOmBjejVkpdFq-Puvt1M_NA7JdoJ7OPbrbx3gTrzN8d77bma7gzMt_fos4CL04CMXxfOS1mdumwk_Uc1mTaXmLTgfovUUInoe_6TMQjkWJIKfL46zUI5uCc2ZnsnDk4Z0CaDHnm-Z9r_J44WZUJr44Ezp955ziaRI498eCyW4sZgvuH_A-sLqtG</recordid><startdate>20080601</startdate><enddate>20080601</enddate><creator>Osman, Abdullah A.</creator><creator>Schrader, Angela D.</creator><creator>Hawkes, Aubrey J.</creator><creator>Akil, Omar</creator><creator>Bergeron, Adam</creator><creator>Lustig, Lawrence R.</creator><creator>Simmons, Dwayne D.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20080601</creationdate><title>Muscle-like nicotinic receptor accessory molecules in sensory hair cells of the inner ear</title><author>Osman, Abdullah A. ; Schrader, Angela D. ; Hawkes, Aubrey J. ; Akil, Omar ; Bergeron, Adam ; Lustig, Lawrence R. ; Simmons, Dwayne D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c480t-9b734506b56c7fc128e8105dadfeeee210ae08911c13cc5e18c138d8c51bf8333</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animals</topic><topic>Bungarotoxins - pharmacology</topic><topic>Calcium - metabolism</topic><topic>Cholinergic synapse</topic><topic>Female</topic><topic>Hair Cells, Auditory, Inner - cytology</topic><topic>Hair Cells, Auditory, Inner - metabolism</topic><topic>Humans</topic><topic>Intracellular Signaling Peptides and Proteins - genetics</topic><topic>Intracellular Signaling Peptides and Proteins - metabolism</topic><topic>Kidney - cytology</topic><topic>LLC-PK1 Cells</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Muscle Proteins - metabolism</topic><topic>Peripheral auditory system</topic><topic>Pregnancy</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Receptor assembly and clustering</topic><topic>Receptor Protein-Tyrosine Kinases - genetics</topic><topic>Receptor Protein-Tyrosine Kinases - metabolism</topic><topic>Receptors, Cholinergic - genetics</topic><topic>Receptors, Cholinergic - metabolism</topic><topic>Receptors, Nicotinic - genetics</topic><topic>Receptors, Nicotinic - metabolism</topic><topic>RNA, Messenger - metabolism</topic><topic>Swine</topic><topic>Synapses - metabolism</topic><topic>Synaptogenesis</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Osman, Abdullah A.</creatorcontrib><creatorcontrib>Schrader, Angela D.</creatorcontrib><creatorcontrib>Hawkes, Aubrey J.</creatorcontrib><creatorcontrib>Akil, Omar</creatorcontrib><creatorcontrib>Bergeron, Adam</creatorcontrib><creatorcontrib>Lustig, Lawrence R.</creatorcontrib><creatorcontrib>Simmons, Dwayne D.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Molecular and cellular neuroscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Osman, Abdullah A.</au><au>Schrader, Angela D.</au><au>Hawkes, Aubrey J.</au><au>Akil, Omar</au><au>Bergeron, Adam</au><au>Lustig, Lawrence R.</au><au>Simmons, Dwayne D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Muscle-like nicotinic receptor accessory molecules in sensory hair cells of the inner ear</atitle><jtitle>Molecular and cellular neuroscience</jtitle><addtitle>Mol Cell Neurosci</addtitle><date>2008-06-01</date><risdate>2008</risdate><volume>38</volume><issue>2</issue><spage>153</spage><epage>169</epage><pages>153-169</pages><issn>1044-7431</issn><eissn>1095-9327</eissn><abstract>Nothing is known about the regulation of nicotinic acetylcholine receptors (nAChRs) in hair cells of the inner ear. MuSK, rapsyn and RIC-3 are accessory molecules associated with muscle and brain nAChR function. We demonstrate that these accessory molecules are expressed in the inner ear raising the possibility of a muscle-like mechanism for clustering and assembly of nAChRs in hair cells. We focused our investigations on rapsyn and RIC-3. Rapsyn interacts with the cytoplasmic loop of nAChR α9 subunits but not nAChR α10 subunits. Although rapsyn and RIC-3 increase nAChR α9 expression, rapsyn plays a greater role in receptor clustering while RIC-3 is important for acetylcholine-induced calcium responses. Our data suggest that RIC-3 facilitates receptor function, while rapsyn enhances receptor clustering at the cell surface.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>18420419</pmid><doi>10.1016/j.mcn.2008.02.008</doi><tpages>17</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Bungarotoxins - pharmacology Calcium - metabolism Cholinergic synapse Female Hair Cells, Auditory, Inner - cytology Hair Cells, Auditory, Inner - metabolism Humans Intracellular Signaling Peptides and Proteins - genetics Intracellular Signaling Peptides and Proteins - metabolism Kidney - cytology LLC-PK1 Cells Male Mice Mice, Inbred C57BL Muscle Proteins - metabolism Peripheral auditory system Pregnancy Rats Rats, Sprague-Dawley Receptor assembly and clustering Receptor Protein-Tyrosine Kinases - genetics Receptor Protein-Tyrosine Kinases - metabolism Receptors, Cholinergic - genetics Receptors, Cholinergic - metabolism Receptors, Nicotinic - genetics Receptors, Nicotinic - metabolism RNA, Messenger - metabolism Swine Synapses - metabolism Synaptogenesis Transfection |
| title | Muscle-like nicotinic receptor accessory molecules in sensory hair cells of the inner ear |
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