Islet cell antibodies represent autoimmune response against several antigens
To study the antigens involved in the islet cell antibody (ICA) reaction we selected 30 patient serum samples (ten in each group) positive for ICA and one other additional autoantibody, such as glutamic acid decarboxylase antibodies (GADA), thyrosine phosphatase antibodies (IA-2A) or insulin autoant...
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| Veröffentlicht in: | International journal of experimental diabetes research 2001, Vol.2 (2), p.85-90 |
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| container_title | International journal of experimental diabetes research |
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| creator | Månsson, L Törn, C Landin-Olsson, M |
| description | To study the antigens involved in the islet cell antibody (ICA) reaction we selected 30 patient serum samples (ten in each group) positive for ICA and one other additional autoantibody, such as glutamic acid decarboxylase antibodies (GADA), thyrosine phosphatase antibodies (IA-2A) or insulin autoantibodies (IAA). The serum samples were incubated with the specific antigen (GAD65, IA-2 or insulin) and the ICA analysis and the corresponding immunoprecipitation assay were performed before and after the absorption. We could then demonstrate that specific autoantibodies against GAD65 and IA-2 could be absorbed with the corresponding antigen, since ten GADA positive and six IA-2A samples turned completely negative. However, the ICA reaction after absorption with GADA, IA-2A and insulin was still present, although at significantly lower levels. The results strongly indicate that the ICA reaction represents simultaneous autoimmunity against several other antigens beside GAD65, IA-2 and insulin. |
| doi_str_mv | 10.1155/EDR.2001.85 |
| format | Article |
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The serum samples were incubated with the specific antigen (GAD65, IA-2 or insulin) and the ICA analysis and the corresponding immunoprecipitation assay were performed before and after the absorption. We could then demonstrate that specific autoantibodies against GAD65 and IA-2 could be absorbed with the corresponding antigen, since ten GADA positive and six IA-2A samples turned completely negative. However, the ICA reaction after absorption with GADA, IA-2A and insulin was still present, although at significantly lower levels. The results strongly indicate that the ICA reaction represents simultaneous autoimmunity against several other antigens beside GAD65, IA-2 and insulin.</description><identifier>ISSN: 1560-4284</identifier><identifier>ISSN: 2314-6745</identifier><identifier>EISSN: 2314-6753</identifier><identifier>DOI: 10.1155/EDR.2001.85</identifier><identifier>PMID: 12369720</identifier><language>eng</language><publisher>United States: Hindawi Publishing Corporation</publisher><subject>Adolescent ; Adult ; Antibody Specificity ; Autoantibodies - blood ; Autoantigens - immunology ; Diabetes Mellitus, Type 1 - blood ; Diabetes Mellitus, Type 1 - immunology ; Glutamate Decarboxylase - immunology ; Humans ; Islets of Langerhans - immunology ; Isoenzymes - immunology ; Membrane Proteins - immunology ; Protein Tyrosine Phosphatase, Non-Receptor Type 1 ; Protein Tyrosine Phosphatases - immunology ; Receptor-Like Protein Tyrosine Phosphatases, Class 8</subject><ispartof>International journal of experimental diabetes research, 2001, Vol.2 (2), p.85-90</ispartof><rights>Copyright © 2001 Hindawi Publishing Corporation. 2001 Hindawi Publishing Corporation</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3235-bbb00b768e068664b703d22436ba3988e6db8f1bd469200329e2f66779279ef03</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2478538/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2478538/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,4009,27902,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12369720$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Månsson, L</creatorcontrib><creatorcontrib>Törn, C</creatorcontrib><creatorcontrib>Landin-Olsson, M</creatorcontrib><title>Islet cell antibodies represent autoimmune response against several antigens</title><title>International journal of experimental diabetes research</title><addtitle>Int J Exp Diabetes Res</addtitle><description>To study the antigens involved in the islet cell antibody (ICA) reaction we selected 30 patient serum samples (ten in each group) positive for ICA and one other additional autoantibody, such as glutamic acid decarboxylase antibodies (GADA), thyrosine phosphatase antibodies (IA-2A) or insulin autoantibodies (IAA). The serum samples were incubated with the specific antigen (GAD65, IA-2 or insulin) and the ICA analysis and the corresponding immunoprecipitation assay were performed before and after the absorption. We could then demonstrate that specific autoantibodies against GAD65 and IA-2 could be absorbed with the corresponding antigen, since ten GADA positive and six IA-2A samples turned completely negative. However, the ICA reaction after absorption with GADA, IA-2A and insulin was still present, although at significantly lower levels. The results strongly indicate that the ICA reaction represents simultaneous autoimmunity against several other antigens beside GAD65, IA-2 and insulin.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Antibody Specificity</subject><subject>Autoantibodies - blood</subject><subject>Autoantigens - immunology</subject><subject>Diabetes Mellitus, Type 1 - blood</subject><subject>Diabetes Mellitus, Type 1 - immunology</subject><subject>Glutamate Decarboxylase - immunology</subject><subject>Humans</subject><subject>Islets of Langerhans - immunology</subject><subject>Isoenzymes - immunology</subject><subject>Membrane Proteins - immunology</subject><subject>Protein Tyrosine Phosphatase, Non-Receptor Type 1</subject><subject>Protein Tyrosine Phosphatases - immunology</subject><subject>Receptor-Like Protein Tyrosine Phosphatases, Class 8</subject><issn>1560-4284</issn><issn>2314-6745</issn><issn>2314-6753</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctLw0AQxhdRbK2evEtOXiR1H9nXRZBatVAQRM_LbjKpkWRTd5OC_70pLT5OngZmvvn4zXwInRM8JYTz6_nd85RiTKaKH6AxZSRLheTsEI0JFzjNqMpG6CTGd4wpxQofoxGhTGhJ8RgtF7GGLsmhrhPru8q1RQUxCbAOEMF3ie27tmqa3sPQjOvWR0jsylY-dkmEDQS7W1yBj6foqLR1hLN9naDX-_nL7DFdPj0sZrfLNGeU8dQ5h7GTQgEWSojMScwKSjMmnGVaKRCFUyVxRSb0cBijGmgphJSaSg0lZhN0s_Nd966BIh84BwyzDlVjw6dpbWX-Tnz1ZlbtxtBMKs7UYHC5NwjtRw-xM00Vtz-wHto-GkkYVZzrf4VES04UloPwaifMQxtjgPKbhmCzjckMMZltTGYgmKCL3wf8aPe5sC_KEo4z</recordid><startdate>2001</startdate><enddate>2001</enddate><creator>Månsson, L</creator><creator>Törn, C</creator><creator>Landin-Olsson, M</creator><general>Hindawi Publishing Corporation</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>2001</creationdate><title>Islet cell antibodies represent autoimmune response against several antigens</title><author>Månsson, L ; Törn, C ; Landin-Olsson, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3235-bbb00b768e068664b703d22436ba3988e6db8f1bd469200329e2f66779279ef03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Antibody Specificity</topic><topic>Autoantibodies - blood</topic><topic>Autoantigens - immunology</topic><topic>Diabetes Mellitus, Type 1 - blood</topic><topic>Diabetes Mellitus, Type 1 - immunology</topic><topic>Glutamate Decarboxylase - immunology</topic><topic>Humans</topic><topic>Islets of Langerhans - immunology</topic><topic>Isoenzymes - immunology</topic><topic>Membrane Proteins - immunology</topic><topic>Protein Tyrosine Phosphatase, Non-Receptor Type 1</topic><topic>Protein Tyrosine Phosphatases - immunology</topic><topic>Receptor-Like Protein Tyrosine Phosphatases, Class 8</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Månsson, L</creatorcontrib><creatorcontrib>Törn, C</creatorcontrib><creatorcontrib>Landin-Olsson, M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International journal of experimental diabetes research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Månsson, L</au><au>Törn, C</au><au>Landin-Olsson, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Islet cell antibodies represent autoimmune response against several antigens</atitle><jtitle>International journal of experimental diabetes research</jtitle><addtitle>Int J Exp Diabetes Res</addtitle><date>2001</date><risdate>2001</risdate><volume>2</volume><issue>2</issue><spage>85</spage><epage>90</epage><pages>85-90</pages><issn>1560-4284</issn><issn>2314-6745</issn><eissn>2314-6753</eissn><abstract>To study the antigens involved in the islet cell antibody (ICA) reaction we selected 30 patient serum samples (ten in each group) positive for ICA and one other additional autoantibody, such as glutamic acid decarboxylase antibodies (GADA), thyrosine phosphatase antibodies (IA-2A) or insulin autoantibodies (IAA). The serum samples were incubated with the specific antigen (GAD65, IA-2 or insulin) and the ICA analysis and the corresponding immunoprecipitation assay were performed before and after the absorption. We could then demonstrate that specific autoantibodies against GAD65 and IA-2 could be absorbed with the corresponding antigen, since ten GADA positive and six IA-2A samples turned completely negative. However, the ICA reaction after absorption with GADA, IA-2A and insulin was still present, although at significantly lower levels. The results strongly indicate that the ICA reaction represents simultaneous autoimmunity against several other antigens beside GAD65, IA-2 and insulin.</abstract><cop>United States</cop><pub>Hindawi Publishing Corporation</pub><pmid>12369720</pmid><doi>10.1155/EDR.2001.85</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; Wiley-Blackwell Open Access Titles; PubMed Central; Alma/SFX Local Collection |
| subjects | Adolescent Adult Antibody Specificity Autoantibodies - blood Autoantigens - immunology Diabetes Mellitus, Type 1 - blood Diabetes Mellitus, Type 1 - immunology Glutamate Decarboxylase - immunology Humans Islets of Langerhans - immunology Isoenzymes - immunology Membrane Proteins - immunology Protein Tyrosine Phosphatase, Non-Receptor Type 1 Protein Tyrosine Phosphatases - immunology Receptor-Like Protein Tyrosine Phosphatases, Class 8 |
| title | Islet cell antibodies represent autoimmune response against several antigens |
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