Distinct horizontal patterns in the spatial organization of superficial zone chondrocytes of human joints
A better understanding of the unique cellular and functional properties of the superficial zone of articular cartilage may aid current strategies in tissue engineering which attempts a layered design for the repair of cartilage lesions to avert or postpone the onset of osteoarthritis. However, data...
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| Veröffentlicht in: | Journal of structural biology 2008-05, Vol.162 (2), p.335-344 |
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| creator | Rolauffs, Bernd Williams, James M. Grodzinsky, Alan J. Kuettner, Klaus E. Cole, Ada A. |
| description | A better understanding of the unique cellular and functional properties of the superficial zone of articular cartilage may aid current strategies in tissue engineering which attempts a layered design for the repair of cartilage lesions to avert or postpone the onset of osteoarthritis. However, data pertaining to the cellular organization of non-degenerated superficial zone of articular cartilage is not available for most human joints. The present study analyzed the arrangement of chondrocytes of non-degenerated human joints (shoulder, elbow, knee, and ankle) by using fluorescence microscopy of the superficial zone in a top-down view. The resulting horizontal chondrocyte arrangements were tested for randomness, homogeneity or a significant grouping via point pattern analysis and were correlated with the joint type in which they occurred. The present study demonstrated that human superficial chondrocytes occurred in four distinct patterns of strings, clusters, pairs or single chondrocytes. Those patterns represented a significant grouping (p |
| doi_str_mv | 10.1016/j.jsb.2008.01.010 |
| format | Article |
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However, data pertaining to the cellular organization of non-degenerated superficial zone of articular cartilage is not available for most human joints. The present study analyzed the arrangement of chondrocytes of non-degenerated human joints (shoulder, elbow, knee, and ankle) by using fluorescence microscopy of the superficial zone in a top-down view. The resulting horizontal chondrocyte arrangements were tested for randomness, homogeneity or a significant grouping via point pattern analysis and were correlated with the joint type in which they occurred. The present study demonstrated that human superficial chondrocytes occurred in four distinct patterns of strings, clusters, pairs or single chondrocytes. Those patterns represented a significant grouping (p<0.0001) with horizontal alignment. Each articular joint surface was dominated by only one of these four patterns (p<0.001). Specific patterns correlated with specific diarthrodial joint types (p<0.001). Further studies need to establish whether these organizational patterns are a consequence of their surrounding environment or whether they are linked to a functional purpose.</description><subject>Ankle</subject><subject>Articular cartilage</subject><subject>Body Patterning</subject><subject>Cartilage, Articular - anatomy & histology</subject><subject>Cartilage, Articular - cytology</subject><subject>Cell organization</subject><subject>Chondrocyte</subject><subject>Chondrocytes - cytology</subject><subject>Chondron</subject><subject>Cluster</subject><subject>Clustering</subject><subject>Human</subject><subject>Humans</subject><subject>Joint type</subject><subject>Joints</subject><subject>Knee</subject><subject>Microscopy, Fluorescence</subject><subject>Point pattern</subject><subject>Superficial zone</subject><issn>1047-8477</issn><issn>1095-8657</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UU1r3DAQFaWl-eoP6CXo1Ju3GllrywQCIW3TQqCX5iy08iiW8UpbSQ4kvz5yd0nTS2FAmpn33gzzCPkIbAUMms_jakybFWdMrhiUYG_IMbBuXclm3b5d_qKtpGjbI3KS0sgYE8DhPTkCWfN1K9tj4r64lJ03mQ4huqfgs57oTueM0SfqPM0D0lQKrtRDvNfePZUkeBosTfMOo3Vm6RUqUjME38dgHjOmBTDMW-3pGJzP6Yy8s3pK-OHwnpK7b19_XX-vbn_e_Li-uq2MEF2uoJe9rVnNetSCSyG0ZQBdCxZ5WR80CCOBd7WRhne6tdrq9QbKDTYdNE1Xn5LLve5u3myxN-hz1JPaRbfV8VEF7dS_He8GdR8eFBeiBsaLwKeDQAy_Z0xZbV0yOE3aY5iTajooZ_wzCfZAE0NKEe3LEGBqMUiNqhikFoMUgxKscM5fb_eXcXCkAC72ACw3enAYVTIOvcHeRTRZ9cH9R_4ZVXukNA</recordid><startdate>20080501</startdate><enddate>20080501</enddate><creator>Rolauffs, Bernd</creator><creator>Williams, James M.</creator><creator>Grodzinsky, Alan J.</creator><creator>Kuettner, Klaus E.</creator><creator>Cole, Ada A.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20080501</creationdate><title>Distinct horizontal patterns in the spatial organization of superficial zone chondrocytes of human joints</title><author>Rolauffs, Bernd ; Williams, James M. ; Grodzinsky, Alan J. ; Kuettner, Klaus E. ; Cole, Ada A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c449t-1d8df3030dea42844af011971fe20411a14c81293c8c29a7fafa5b1008b916693</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Ankle</topic><topic>Articular cartilage</topic><topic>Body Patterning</topic><topic>Cartilage, Articular - anatomy & histology</topic><topic>Cartilage, Articular - cytology</topic><topic>Cell organization</topic><topic>Chondrocyte</topic><topic>Chondrocytes - cytology</topic><topic>Chondron</topic><topic>Cluster</topic><topic>Clustering</topic><topic>Human</topic><topic>Humans</topic><topic>Joint type</topic><topic>Joints</topic><topic>Knee</topic><topic>Microscopy, Fluorescence</topic><topic>Point pattern</topic><topic>Superficial zone</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rolauffs, Bernd</creatorcontrib><creatorcontrib>Williams, James M.</creatorcontrib><creatorcontrib>Grodzinsky, Alan J.</creatorcontrib><creatorcontrib>Kuettner, Klaus E.</creatorcontrib><creatorcontrib>Cole, Ada A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of structural biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rolauffs, Bernd</au><au>Williams, James M.</au><au>Grodzinsky, Alan J.</au><au>Kuettner, Klaus E.</au><au>Cole, Ada A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Distinct horizontal patterns in the spatial organization of superficial zone chondrocytes of human joints</atitle><jtitle>Journal of structural biology</jtitle><addtitle>J Struct Biol</addtitle><date>2008-05-01</date><risdate>2008</risdate><volume>162</volume><issue>2</issue><spage>335</spage><epage>344</epage><pages>335-344</pages><issn>1047-8477</issn><eissn>1095-8657</eissn><abstract>A better understanding of the unique cellular and functional properties of the superficial zone of articular cartilage may aid current strategies in tissue engineering which attempts a layered design for the repair of cartilage lesions to avert or postpone the onset of osteoarthritis. However, data pertaining to the cellular organization of non-degenerated superficial zone of articular cartilage is not available for most human joints. The present study analyzed the arrangement of chondrocytes of non-degenerated human joints (shoulder, elbow, knee, and ankle) by using fluorescence microscopy of the superficial zone in a top-down view. The resulting horizontal chondrocyte arrangements were tested for randomness, homogeneity or a significant grouping via point pattern analysis and were correlated with the joint type in which they occurred. The present study demonstrated that human superficial chondrocytes occurred in four distinct patterns of strings, clusters, pairs or single chondrocytes. Those patterns represented a significant grouping (p<0.0001) with horizontal alignment. Each articular joint surface was dominated by only one of these four patterns (p<0.001). Specific patterns correlated with specific diarthrodial joint types (p<0.001). 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| ispartof | Journal of structural biology, 2008-05, Vol.162 (2), p.335-344 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Ankle Articular cartilage Body Patterning Cartilage, Articular - anatomy & histology Cartilage, Articular - cytology Cell organization Chondrocyte Chondrocytes - cytology Chondron Cluster Clustering Human Humans Joint type Joints Knee Microscopy, Fluorescence Point pattern Superficial zone |
| title | Distinct horizontal patterns in the spatial organization of superficial zone chondrocytes of human joints |
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