Corneal response to femtosecond laser photodisruption in the rabbit
In this report we evaluated the effect of femtosecond laser energy on the development of corneal haze and keratocyte activation in rabbits following intra-stromal photodisruption to create LASIK flaps using a modified commercial femtosecond surgical laser. Three groups of flap parameters were studie...
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| Veröffentlicht in: | Experimental eye research 2008-05, Vol.86 (5), p.835-843 |
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| creator | Morishige, Naoyuki Kesler-Diaz, Anna Wahlert, Andrew J. Kurtz, Ronald M. Juhasz, Tibor Sarayba, Melvin Jester, James V. |
| description | In this report we evaluated the effect of femtosecond laser energy on the development of corneal haze and keratocyte activation in rabbits following intra-stromal photodisruption to create LASIK flaps using a modified commercial femtosecond surgical laser. Three groups of flap parameters were studied: 1.5μJ/pulse with 10μm spot separation and complete side cut (Group 1); 3.5μJ/pulse with 14μm spot separation and complete side cut (Group 2); 3.5μJ/pulse with 14μm spot separation and partial (50μm) side cut (Group 3). All flaps were left attached without lifting to avoid epithelial contamination. Rabbits were then evaluated pre- and post-operatively by quantitative in vivo and ex vivo confocal microscopy. The achieved flap thickness 1week after surgery averaged 88.9±12.8, 90.8±6.9 and 86.5±6.8μm for Groups 1–3 respectively (p=NS). Interface thickness was significantly greater (p |
| doi_str_mv | 10.1016/j.exer.2008.02.012 |
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Three groups of flap parameters were studied: 1.5μJ/pulse with 10μm spot separation and complete side cut (Group 1); 3.5μJ/pulse with 14μm spot separation and complete side cut (Group 2); 3.5μJ/pulse with 14μm spot separation and partial (50μm) side cut (Group 3). All flaps were left attached without lifting to avoid epithelial contamination. Rabbits were then evaluated pre- and post-operatively by quantitative in vivo and ex vivo confocal microscopy. The achieved flap thickness 1week after surgery averaged 88.9±12.8, 90.8±6.9 and 86.5±6.8μm for Groups 1–3 respectively (p=NS). Interface thickness was significantly greater (p<0.05) in the higher energy groups averaging 40.0±11.2 and 37.7±5.7μm for Groups 2–3 compared to 28.6±4.5μm for Group 1. Corneal haze was barely detectible and not significantly different between groups, although haze was detected in the region of the side-cuts in Groups 1 and 2. No clinically significant changes in stromal or epithelial thickness were noted. Laser confocal microscopy showed the presence of small diameter cells within the flap interface that resided within disrupted regions of the corneal collagen lamellae. Keratocyte activation was only detected in regions of the 100% side cut and not over the flap interface. In conclusion, the results of this study indicate that photodisruption of the corneal stroma alone without flap elevation regardless of laser energy does not induce significant corneal haze in the rabbit. However, a thicker stromal interface was seen with the higher energy suggesting greater stromal damage.</description><identifier>ISSN: 0014-4835</identifier><identifier>EISSN: 1096-0007</identifier><identifier>DOI: 10.1016/j.exer.2008.02.012</identifier><identifier>PMID: 18395714</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; corneal haze ; Corneal Opacity - etiology ; Corneal Opacity - pathology ; Corneal Stroma - pathology ; Corneal Stroma - surgery ; Epithelium, Corneal - pathology ; Image Processing, Computer-Assisted - methods ; in vivo confocal microscopy ; IntraLase ; keratocyte activation ; Keratomileusis, Laser In Situ - adverse effects ; Keratomileusis, Laser In Situ - methods ; LASIK ; Microscopy, Confocal ; Postoperative Period ; Rabbits ; Scattering, Radiation ; Wound Healing</subject><ispartof>Experimental eye research, 2008-05, Vol.86 (5), p.835-843</ispartof><rights>2008 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c453t-3b6417de302a77f4d45bb79895e35fb54b49ce66633a62f452764b97cb261aa73</citedby><cites>FETCH-LOGICAL-c453t-3b6417de302a77f4d45bb79895e35fb54b49ce66633a62f452764b97cb261aa73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0014483508000717$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18395714$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Morishige, Naoyuki</creatorcontrib><creatorcontrib>Kesler-Diaz, Anna</creatorcontrib><creatorcontrib>Wahlert, Andrew J.</creatorcontrib><creatorcontrib>Kurtz, Ronald M.</creatorcontrib><creatorcontrib>Juhasz, Tibor</creatorcontrib><creatorcontrib>Sarayba, Melvin</creatorcontrib><creatorcontrib>Jester, James V.</creatorcontrib><title>Corneal response to femtosecond laser photodisruption in the rabbit</title><title>Experimental eye research</title><addtitle>Exp Eye Res</addtitle><description>In this report we evaluated the effect of femtosecond laser energy on the development of corneal haze and keratocyte activation in rabbits following intra-stromal photodisruption to create LASIK flaps using a modified commercial femtosecond surgical laser. Three groups of flap parameters were studied: 1.5μJ/pulse with 10μm spot separation and complete side cut (Group 1); 3.5μJ/pulse with 14μm spot separation and complete side cut (Group 2); 3.5μJ/pulse with 14μm spot separation and partial (50μm) side cut (Group 3). All flaps were left attached without lifting to avoid epithelial contamination. Rabbits were then evaluated pre- and post-operatively by quantitative in vivo and ex vivo confocal microscopy. The achieved flap thickness 1week after surgery averaged 88.9±12.8, 90.8±6.9 and 86.5±6.8μm for Groups 1–3 respectively (p=NS). Interface thickness was significantly greater (p<0.05) in the higher energy groups averaging 40.0±11.2 and 37.7±5.7μm for Groups 2–3 compared to 28.6±4.5μm for Group 1. Corneal haze was barely detectible and not significantly different between groups, although haze was detected in the region of the side-cuts in Groups 1 and 2. No clinically significant changes in stromal or epithelial thickness were noted. Laser confocal microscopy showed the presence of small diameter cells within the flap interface that resided within disrupted regions of the corneal collagen lamellae. Keratocyte activation was only detected in regions of the 100% side cut and not over the flap interface. In conclusion, the results of this study indicate that photodisruption of the corneal stroma alone without flap elevation regardless of laser energy does not induce significant corneal haze in the rabbit. However, a thicker stromal interface was seen with the higher energy suggesting greater stromal damage.</description><subject>Animals</subject><subject>corneal haze</subject><subject>Corneal Opacity - etiology</subject><subject>Corneal Opacity - pathology</subject><subject>Corneal Stroma - pathology</subject><subject>Corneal Stroma - surgery</subject><subject>Epithelium, Corneal - pathology</subject><subject>Image Processing, Computer-Assisted - methods</subject><subject>in vivo confocal microscopy</subject><subject>IntraLase</subject><subject>keratocyte activation</subject><subject>Keratomileusis, Laser In Situ - adverse effects</subject><subject>Keratomileusis, Laser In Situ - methods</subject><subject>LASIK</subject><subject>Microscopy, Confocal</subject><subject>Postoperative Period</subject><subject>Rabbits</subject><subject>Scattering, Radiation</subject><subject>Wound Healing</subject><issn>0014-4835</issn><issn>1096-0007</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1r3DAQhkVJSLZJ_kAPxafe7OpbNoRCWPoFgV6Ss5DkcVeLV3IkbWj_fbXs0qaXMIeBmfd9Z3gQekdwRzCRH7cd_ILUUYz7DtMOE_oGrQgeZIsxVmdohTHhLe-ZuERvc97WKeOKX6BL0rNBKMJXaL2OKYCZmwR5iSFDU2Izwa7EDC6GsZlNhtQsm1ji6HPaL8XH0PjQlA00yVjryzU6n8yc4ebUr9Djl88P62_t_Y-v39d3963jgpWWWcmJGoFhapSa-MiFtWroBwFMTFZwywcHUkrGjKQTF1RJbgflLJXEGMWu0Kdj7rK3OxgdhJLMrJfkdyb91tF4_f8m-I3-GZ815Zz0StSAD6eAFJ_2kIve-exgnk2AuM9aDmTgtaqQHoUuxZwTTH-PEKwP7PVWH9jrA3uNqa7sq-n9y_f-WU6wq-D2KIAK6dlXe3YegoPRJ3BFj9G_lv8HuvWW8g</recordid><startdate>20080501</startdate><enddate>20080501</enddate><creator>Morishige, Naoyuki</creator><creator>Kesler-Diaz, Anna</creator><creator>Wahlert, Andrew J.</creator><creator>Kurtz, Ronald M.</creator><creator>Juhasz, Tibor</creator><creator>Sarayba, Melvin</creator><creator>Jester, James V.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20080501</creationdate><title>Corneal response to femtosecond laser photodisruption in the rabbit</title><author>Morishige, Naoyuki ; Kesler-Diaz, Anna ; Wahlert, Andrew J. ; Kurtz, Ronald M. ; Juhasz, Tibor ; Sarayba, Melvin ; Jester, James V.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c453t-3b6417de302a77f4d45bb79895e35fb54b49ce66633a62f452764b97cb261aa73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animals</topic><topic>corneal haze</topic><topic>Corneal Opacity - etiology</topic><topic>Corneal Opacity - pathology</topic><topic>Corneal Stroma - pathology</topic><topic>Corneal Stroma - surgery</topic><topic>Epithelium, Corneal - pathology</topic><topic>Image Processing, Computer-Assisted - methods</topic><topic>in vivo confocal microscopy</topic><topic>IntraLase</topic><topic>keratocyte activation</topic><topic>Keratomileusis, Laser In Situ - adverse effects</topic><topic>Keratomileusis, Laser In Situ - methods</topic><topic>LASIK</topic><topic>Microscopy, Confocal</topic><topic>Postoperative Period</topic><topic>Rabbits</topic><topic>Scattering, Radiation</topic><topic>Wound Healing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Morishige, Naoyuki</creatorcontrib><creatorcontrib>Kesler-Diaz, Anna</creatorcontrib><creatorcontrib>Wahlert, Andrew J.</creatorcontrib><creatorcontrib>Kurtz, Ronald M.</creatorcontrib><creatorcontrib>Juhasz, Tibor</creatorcontrib><creatorcontrib>Sarayba, Melvin</creatorcontrib><creatorcontrib>Jester, James V.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Experimental eye research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Morishige, Naoyuki</au><au>Kesler-Diaz, Anna</au><au>Wahlert, Andrew J.</au><au>Kurtz, Ronald M.</au><au>Juhasz, Tibor</au><au>Sarayba, Melvin</au><au>Jester, James V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Corneal response to femtosecond laser photodisruption in the rabbit</atitle><jtitle>Experimental eye research</jtitle><addtitle>Exp Eye Res</addtitle><date>2008-05-01</date><risdate>2008</risdate><volume>86</volume><issue>5</issue><spage>835</spage><epage>843</epage><pages>835-843</pages><issn>0014-4835</issn><eissn>1096-0007</eissn><abstract>In this report we evaluated the effect of femtosecond laser energy on the development of corneal haze and keratocyte activation in rabbits following intra-stromal photodisruption to create LASIK flaps using a modified commercial femtosecond surgical laser. Three groups of flap parameters were studied: 1.5μJ/pulse with 10μm spot separation and complete side cut (Group 1); 3.5μJ/pulse with 14μm spot separation and complete side cut (Group 2); 3.5μJ/pulse with 14μm spot separation and partial (50μm) side cut (Group 3). All flaps were left attached without lifting to avoid epithelial contamination. Rabbits were then evaluated pre- and post-operatively by quantitative in vivo and ex vivo confocal microscopy. The achieved flap thickness 1week after surgery averaged 88.9±12.8, 90.8±6.9 and 86.5±6.8μm for Groups 1–3 respectively (p=NS). Interface thickness was significantly greater (p<0.05) in the higher energy groups averaging 40.0±11.2 and 37.7±5.7μm for Groups 2–3 compared to 28.6±4.5μm for Group 1. Corneal haze was barely detectible and not significantly different between groups, although haze was detected in the region of the side-cuts in Groups 1 and 2. No clinically significant changes in stromal or epithelial thickness were noted. Laser confocal microscopy showed the presence of small diameter cells within the flap interface that resided within disrupted regions of the corneal collagen lamellae. Keratocyte activation was only detected in regions of the 100% side cut and not over the flap interface. In conclusion, the results of this study indicate that photodisruption of the corneal stroma alone without flap elevation regardless of laser energy does not induce significant corneal haze in the rabbit. However, a thicker stromal interface was seen with the higher energy suggesting greater stromal damage.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>18395714</pmid><doi>10.1016/j.exer.2008.02.012</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals corneal haze Corneal Opacity - etiology Corneal Opacity - pathology Corneal Stroma - pathology Corneal Stroma - surgery Epithelium, Corneal - pathology Image Processing, Computer-Assisted - methods in vivo confocal microscopy IntraLase keratocyte activation Keratomileusis, Laser In Situ - adverse effects Keratomileusis, Laser In Situ - methods LASIK Microscopy, Confocal Postoperative Period Rabbits Scattering, Radiation Wound Healing |
| title | Corneal response to femtosecond laser photodisruption in the rabbit |
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