Docosahexaenoic Acid Enhances Segregation of Lipids between Raft and Nonraft Domains: 2H-NMR Study
Solid-state 2H-NMR of [2H31]-N-palmitoylsphingomyelin ([2H31]16:0SM, PSM*), supplemented by differential scanning calorimetry, was used for the first time, to our knowledge, to investigate the molecular organization of the sphingolipid in 1:1:1mol mixtures with 1-palmitoyl-2-oleoyl-sn-glycero-3-phos...
Gespeichert in:
| Veröffentlicht in: | Biophysical journal 2008-07, Vol.95 (1), p.203-214 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 214 |
|---|---|
| container_issue | 1 |
| container_start_page | 203 |
| container_title | Biophysical journal |
| container_volume | 95 |
| creator | Soni, Smita P. LoCascio, Daniel S. Liu, Yidong Williams, Justin A. Bittman, Robert Stillwell, William Wassall, Stephen R. |
| description | Solid-state 2H-NMR of [2H31]-N-palmitoylsphingomyelin ([2H31]16:0SM, PSM*), supplemented by differential scanning calorimetry, was used for the first time, to our knowledge, to investigate the molecular organization of the sphingolipid in 1:1:1mol mixtures with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (16:0–18:1PE, POPE) or 1-palmitoyl-2-docosahexaenoyl-sn-glycero-3-phosphoethanolamine (16:0–22:6PE, PDPE) and cholesterol. When compared with 2H-NMR data for analogous mixtures of [2H31]16:0–18:1PE (POPE*) or [2H31]16:0-22:6PE (PDPE*) with egg SM and cholesterol, molecular interactions of oleic acid (OA) versus docosahexaenoic acid (DHA) are distinguished, and details of membrane architecture emerge. SM-rich, characterized by higher-order, and PE-rich, characterized by lower-order, domains |
| doi_str_mv | 10.1529/biophysj.107.123612 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2426647</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0006349508702960</els_id><sourcerecordid>864419400</sourcerecordid><originalsourceid>FETCH-LOGICAL-c2802-a3b7e71d0347f140ccb1b494226f1762b5449ab0fe6df6f93c642506f2c4c2013</originalsourceid><addsrcrecordid>eNp9UU1vEzEQtRCIhra_gItvnDaMP9abRQKpagtFSlupH2fLH-PEVWIHe1PIv2erlEq9cJrRzHtvNO8R8pHBlLW8_2xj3ix39WHKoJsyLhTjb8iEtZI3ADP1lkwAQDVC9u0B-VDrAwDjLbD35IDNhOg7ySfEnmWXq1niH4MpR0dPXPT0PC1NcljpLS4KLswQc6I50HncRF-pxeE3YqI3JgzUJE-vcipP_Vlem5jqF8ovmqvLG3o7bP3uiLwLZlXx-Lkekvvv53enF838-sfP05N54_gMeGOE7bBjHoTsApPgnGVW9pJzFVinuG2l7I2FgMoHFXrhlBzfUYE76TgwcUi-7XU3W7tG7zANxaz0psS1KTudTdSvNyku9SI_ai65UrIbBT49C5T8a4t10OtYHa5WJmHeVj1TUrJeAoxIsUe6kmstGF6uMNBP4eh_4YyDTu_DGVlf9ywcXXiMWHR1EUeffSzoBu1z_C__L9OjmJY</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>864419400</pqid></control><display><type>article</type><title>Docosahexaenoic Acid Enhances Segregation of Lipids between Raft and Nonraft Domains: 2H-NMR Study</title><source>Cell Press Free Archives</source><source>Access via ScienceDirect (Elsevier)</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>Soni, Smita P. ; LoCascio, Daniel S. ; Liu, Yidong ; Williams, Justin A. ; Bittman, Robert ; Stillwell, William ; Wassall, Stephen R.</creator><creatorcontrib>Soni, Smita P. ; LoCascio, Daniel S. ; Liu, Yidong ; Williams, Justin A. ; Bittman, Robert ; Stillwell, William ; Wassall, Stephen R.</creatorcontrib><description>Solid-state 2H-NMR of [2H31]-N-palmitoylsphingomyelin ([2H31]16:0SM, PSM*), supplemented by differential scanning calorimetry, was used for the first time, to our knowledge, to investigate the molecular organization of the sphingolipid in 1:1:1mol mixtures with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (16:0–18:1PE, POPE) or 1-palmitoyl-2-docosahexaenoyl-sn-glycero-3-phosphoethanolamine (16:0–22:6PE, PDPE) and cholesterol. When compared with 2H-NMR data for analogous mixtures of [2H31]16:0–18:1PE (POPE*) or [2H31]16:0-22:6PE (PDPE*) with egg SM and cholesterol, molecular interactions of oleic acid (OA) versus docosahexaenoic acid (DHA) are distinguished, and details of membrane architecture emerge. SM-rich, characterized by higher-order, and PE-rich, characterized by lower-order, domains <20nm in size are formed in the absence and presence of cholesterol in both OA- and DHA-containing membranes. Although acyl chain order within both domains increases on the addition of sterol to the two systems, the resultant differential in order between SM- and PE-rich domains is almost a factor of 3 greater with DHA than with OA. Our interpretation is that the aversion that cholesterol has for DHA—but not for OA—excludes the sterol from DHA-containing, PE-rich (nonraft) domains and excludes DHA from SM-rich/cholesterol-rich (raft) domains. We attribute, in part, the diverse health benefits associated with dietary consumption of DHA to an alteration in membrane domains.</description><identifier>ISSN: 0006-3495</identifier><identifier>EISSN: 1542-0086</identifier><identifier>DOI: 10.1529/biophysj.107.123612</identifier><identifier>PMID: 18339742</identifier><language>eng</language><publisher>Elsevier Inc</publisher><subject>Cholesterol ; Differential scanning calorimetry ; Docosahexaenoic acid ; Lipids ; Membranes ; Molecular interactions ; Rafts ; Sterols</subject><ispartof>Biophysical journal, 2008-07, Vol.95 (1), p.203-214</ispartof><rights>2008 The Biophysical Society</rights><rights>Copyright © 2008, Biophysical Society</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2802-a3b7e71d0347f140ccb1b494226f1762b5449ab0fe6df6f93c642506f2c4c2013</citedby><cites>FETCH-LOGICAL-c2802-a3b7e71d0347f140ccb1b494226f1762b5449ab0fe6df6f93c642506f2c4c2013</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2426647/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://dx.doi.org/10.1529/biophysj.107.123612$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,3550,27924,27925,45995,53791,53793</link.rule.ids></links><search><creatorcontrib>Soni, Smita P.</creatorcontrib><creatorcontrib>LoCascio, Daniel S.</creatorcontrib><creatorcontrib>Liu, Yidong</creatorcontrib><creatorcontrib>Williams, Justin A.</creatorcontrib><creatorcontrib>Bittman, Robert</creatorcontrib><creatorcontrib>Stillwell, William</creatorcontrib><creatorcontrib>Wassall, Stephen R.</creatorcontrib><title>Docosahexaenoic Acid Enhances Segregation of Lipids between Raft and Nonraft Domains: 2H-NMR Study</title><title>Biophysical journal</title><description>Solid-state 2H-NMR of [2H31]-N-palmitoylsphingomyelin ([2H31]16:0SM, PSM*), supplemented by differential scanning calorimetry, was used for the first time, to our knowledge, to investigate the molecular organization of the sphingolipid in 1:1:1mol mixtures with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (16:0–18:1PE, POPE) or 1-palmitoyl-2-docosahexaenoyl-sn-glycero-3-phosphoethanolamine (16:0–22:6PE, PDPE) and cholesterol. When compared with 2H-NMR data for analogous mixtures of [2H31]16:0–18:1PE (POPE*) or [2H31]16:0-22:6PE (PDPE*) with egg SM and cholesterol, molecular interactions of oleic acid (OA) versus docosahexaenoic acid (DHA) are distinguished, and details of membrane architecture emerge. SM-rich, characterized by higher-order, and PE-rich, characterized by lower-order, domains <20nm in size are formed in the absence and presence of cholesterol in both OA- and DHA-containing membranes. Although acyl chain order within both domains increases on the addition of sterol to the two systems, the resultant differential in order between SM- and PE-rich domains is almost a factor of 3 greater with DHA than with OA. Our interpretation is that the aversion that cholesterol has for DHA—but not for OA—excludes the sterol from DHA-containing, PE-rich (nonraft) domains and excludes DHA from SM-rich/cholesterol-rich (raft) domains. We attribute, in part, the diverse health benefits associated with dietary consumption of DHA to an alteration in membrane domains.</description><subject>Cholesterol</subject><subject>Differential scanning calorimetry</subject><subject>Docosahexaenoic acid</subject><subject>Lipids</subject><subject>Membranes</subject><subject>Molecular interactions</subject><subject>Rafts</subject><subject>Sterols</subject><issn>0006-3495</issn><issn>1542-0086</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><recordid>eNp9UU1vEzEQtRCIhra_gItvnDaMP9abRQKpagtFSlupH2fLH-PEVWIHe1PIv2erlEq9cJrRzHtvNO8R8pHBlLW8_2xj3ix39WHKoJsyLhTjb8iEtZI3ADP1lkwAQDVC9u0B-VDrAwDjLbD35IDNhOg7ySfEnmWXq1niH4MpR0dPXPT0PC1NcljpLS4KLswQc6I50HncRF-pxeE3YqI3JgzUJE-vcipP_Vlem5jqF8ovmqvLG3o7bP3uiLwLZlXx-Lkekvvv53enF838-sfP05N54_gMeGOE7bBjHoTsApPgnGVW9pJzFVinuG2l7I2FgMoHFXrhlBzfUYE76TgwcUi-7XU3W7tG7zANxaz0psS1KTudTdSvNyku9SI_ai65UrIbBT49C5T8a4t10OtYHa5WJmHeVj1TUrJeAoxIsUe6kmstGF6uMNBP4eh_4YyDTu_DGVlf9ywcXXiMWHR1EUeffSzoBu1z_C__L9OjmJY</recordid><startdate>20080701</startdate><enddate>20080701</enddate><creator>Soni, Smita P.</creator><creator>LoCascio, Daniel S.</creator><creator>Liu, Yidong</creator><creator>Williams, Justin A.</creator><creator>Bittman, Robert</creator><creator>Stillwell, William</creator><creator>Wassall, Stephen R.</creator><general>Elsevier Inc</general><general>The Biophysical Society</general><scope>6I.</scope><scope>AAFTH</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TB</scope><scope>7U5</scope><scope>8FD</scope><scope>FR3</scope><scope>L7M</scope><scope>5PM</scope></search><sort><creationdate>20080701</creationdate><title>Docosahexaenoic Acid Enhances Segregation of Lipids between Raft and Nonraft Domains: 2H-NMR Study</title><author>Soni, Smita P. ; LoCascio, Daniel S. ; Liu, Yidong ; Williams, Justin A. ; Bittman, Robert ; Stillwell, William ; Wassall, Stephen R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2802-a3b7e71d0347f140ccb1b494226f1762b5449ab0fe6df6f93c642506f2c4c2013</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Cholesterol</topic><topic>Differential scanning calorimetry</topic><topic>Docosahexaenoic acid</topic><topic>Lipids</topic><topic>Membranes</topic><topic>Molecular interactions</topic><topic>Rafts</topic><topic>Sterols</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Soni, Smita P.</creatorcontrib><creatorcontrib>LoCascio, Daniel S.</creatorcontrib><creatorcontrib>Liu, Yidong</creatorcontrib><creatorcontrib>Williams, Justin A.</creatorcontrib><creatorcontrib>Bittman, Robert</creatorcontrib><creatorcontrib>Stillwell, William</creatorcontrib><creatorcontrib>Wassall, Stephen R.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>CrossRef</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Biophysical journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Soni, Smita P.</au><au>LoCascio, Daniel S.</au><au>Liu, Yidong</au><au>Williams, Justin A.</au><au>Bittman, Robert</au><au>Stillwell, William</au><au>Wassall, Stephen R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Docosahexaenoic Acid Enhances Segregation of Lipids between Raft and Nonraft Domains: 2H-NMR Study</atitle><jtitle>Biophysical journal</jtitle><date>2008-07-01</date><risdate>2008</risdate><volume>95</volume><issue>1</issue><spage>203</spage><epage>214</epage><pages>203-214</pages><issn>0006-3495</issn><eissn>1542-0086</eissn><abstract>Solid-state 2H-NMR of [2H31]-N-palmitoylsphingomyelin ([2H31]16:0SM, PSM*), supplemented by differential scanning calorimetry, was used for the first time, to our knowledge, to investigate the molecular organization of the sphingolipid in 1:1:1mol mixtures with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (16:0–18:1PE, POPE) or 1-palmitoyl-2-docosahexaenoyl-sn-glycero-3-phosphoethanolamine (16:0–22:6PE, PDPE) and cholesterol. When compared with 2H-NMR data for analogous mixtures of [2H31]16:0–18:1PE (POPE*) or [2H31]16:0-22:6PE (PDPE*) with egg SM and cholesterol, molecular interactions of oleic acid (OA) versus docosahexaenoic acid (DHA) are distinguished, and details of membrane architecture emerge. SM-rich, characterized by higher-order, and PE-rich, characterized by lower-order, domains <20nm in size are formed in the absence and presence of cholesterol in both OA- and DHA-containing membranes. Although acyl chain order within both domains increases on the addition of sterol to the two systems, the resultant differential in order between SM- and PE-rich domains is almost a factor of 3 greater with DHA than with OA. Our interpretation is that the aversion that cholesterol has for DHA—but not for OA—excludes the sterol from DHA-containing, PE-rich (nonraft) domains and excludes DHA from SM-rich/cholesterol-rich (raft) domains. We attribute, in part, the diverse health benefits associated with dietary consumption of DHA to an alteration in membrane domains.</abstract><pub>Elsevier Inc</pub><pmid>18339742</pmid><doi>10.1529/biophysj.107.123612</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-3495 |
| ispartof | Biophysical journal, 2008-07, Vol.95 (1), p.203-214 |
| issn | 0006-3495 1542-0086 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2426647 |
| source | Cell Press Free Archives; Access via ScienceDirect (Elsevier); EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Cholesterol Differential scanning calorimetry Docosahexaenoic acid Lipids Membranes Molecular interactions Rafts Sterols |
| title | Docosahexaenoic Acid Enhances Segregation of Lipids between Raft and Nonraft Domains: 2H-NMR Study |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-27T01%3A40%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Docosahexaenoic%20Acid%20Enhances%20Segregation%20of%20Lipids%20between%20Raft%20and%20Nonraft%20Domains:%202H-NMR%20Study&rft.jtitle=Biophysical%20journal&rft.au=Soni,%20Smita%20P.&rft.date=2008-07-01&rft.volume=95&rft.issue=1&rft.spage=203&rft.epage=214&rft.pages=203-214&rft.issn=0006-3495&rft.eissn=1542-0086&rft_id=info:doi/10.1529/biophysj.107.123612&rft_dat=%3Cproquest_pubme%3E864419400%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=864419400&rft_id=info:pmid/18339742&rft_els_id=S0006349508702960&rfr_iscdi=true |