Human T-cell Leukemia Virus Type 1 Oncoprotein Tax Represses ZNF268 Expression through the cAMP-responsive Element-binding Protein/Activating Transcription Factor Pathway
Expression of the human T-cell leukemia virus type 1 (HTLV-1) oncoprotein Tax is correlated with cellular transformation, contributing to the development of adult T-cell leukemia. In this study, we investigated the role of Tax in the regulation of the ZNF268 gene, which plays a role in the different...
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| Veröffentlicht in: | The Journal of biological chemistry 2008-06, Vol.283 (24), p.16299-16308 |
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| creator | Wang, Di Guo, Ming-Xiong Hu, Hai-Ming Zhao, Zhou-Zhou Qiu, Hong-Ling Shao, Huan-Jie Zhu, Chen-Gang Xue, Lu Shi, Yun-Bo Li, Wen-Xin |
| description | Expression of the human T-cell leukemia virus type 1 (HTLV-1) oncoprotein Tax is correlated with cellular transformation, contributing to the development of adult T-cell leukemia. In this study, we investigated the role of Tax in the regulation of the ZNF268 gene, which plays a role in the differentiation of blood cells and the pathogenesis of leukemia. We demonstrated that ZNF268 mRNA was repressed in HTLV-1-infected cells. We also showed that stable and transient expression of HTLV-1 Tax led to repression of ZNF268. In addition, by using reporter constructs that bear the human ZNF268 promoter and its mutants, we showed that Tax repressed ZNF268 promoter in a process dependent on a functional cAMP-responsive element. By using Tax, cAMP-responsive element-binding protein (CREB)-1, CREB-2, and their mutants, we further showed that Tax repressed ZNF268 through the CREB/activating transcription factor pathway. Electrophoretic mobility shift assays and chromatin immunoprecipitation demonstrated the formation of the complex of Tax·CREB-1 directly at the cAMP-responsive element both in vitro and in vivo. These findings suggest a role for ZNF268 in aberrant T-cell proliferation observed in HTLV-1-associated diseases. |
| doi_str_mv | 10.1074/jbc.M706426200 |
| format | Article |
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In this study, we investigated the role of Tax in the regulation of the ZNF268 gene, which plays a role in the differentiation of blood cells and the pathogenesis of leukemia. We demonstrated that ZNF268 mRNA was repressed in HTLV-1-infected cells. We also showed that stable and transient expression of HTLV-1 Tax led to repression of ZNF268. In addition, by using reporter constructs that bear the human ZNF268 promoter and its mutants, we showed that Tax repressed ZNF268 promoter in a process dependent on a functional cAMP-responsive element. By using Tax, cAMP-responsive element-binding protein (CREB)-1, CREB-2, and their mutants, we further showed that Tax repressed ZNF268 through the CREB/activating transcription factor pathway. Electrophoretic mobility shift assays and chromatin immunoprecipitation demonstrated the formation of the complex of Tax·CREB-1 directly at the cAMP-responsive element both in vitro and in vivo. These findings suggest a role for ZNF268 in aberrant T-cell proliferation observed in HTLV-1-associated diseases.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1074/jbc.M706426200</identifier><identifier>PMID: 18375384</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Activating Transcription Factor 1 - metabolism ; Base Sequence ; Cyclic AMP - metabolism ; Cyclic AMP Response Element-Binding Protein - metabolism ; DNA-Binding Proteins - metabolism ; Gene Expression Regulation ; Gene Products, tax - physiology ; Human T-lymphotropic virus 1 ; Human T-lymphotropic virus 1 - metabolism ; Humans ; Mechanisms of Signal Transduction ; Models, Biological ; Models, Genetic ; Molecular Sequence Data ; Mutation ; Promoter Regions, Genetic ; Protein Binding ; Repressor Proteins - metabolism</subject><ispartof>The Journal of biological chemistry, 2008-06, Vol.283 (24), p.16299-16308</ispartof><rights>2008 © 2008 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology.</rights><rights>Copyright © 2008, The American Society for Biochemistry and Molecular Biology, Inc. 2008</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c521t-ef7354509e71c173ad4c7abd8860eb1809fc47ed7046dd66d31832484c69b8b93</citedby><cites>FETCH-LOGICAL-c521t-ef7354509e71c173ad4c7abd8860eb1809fc47ed7046dd66d31832484c69b8b93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2423250/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2423250/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18375384$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Di</creatorcontrib><creatorcontrib>Guo, Ming-Xiong</creatorcontrib><creatorcontrib>Hu, Hai-Ming</creatorcontrib><creatorcontrib>Zhao, Zhou-Zhou</creatorcontrib><creatorcontrib>Qiu, Hong-Ling</creatorcontrib><creatorcontrib>Shao, Huan-Jie</creatorcontrib><creatorcontrib>Zhu, Chen-Gang</creatorcontrib><creatorcontrib>Xue, Lu</creatorcontrib><creatorcontrib>Shi, Yun-Bo</creatorcontrib><creatorcontrib>Li, Wen-Xin</creatorcontrib><title>Human T-cell Leukemia Virus Type 1 Oncoprotein Tax Represses ZNF268 Expression through the cAMP-responsive Element-binding Protein/Activating Transcription Factor Pathway</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>Expression of the human T-cell leukemia virus type 1 (HTLV-1) oncoprotein Tax is correlated with cellular transformation, contributing to the development of adult T-cell leukemia. In this study, we investigated the role of Tax in the regulation of the ZNF268 gene, which plays a role in the differentiation of blood cells and the pathogenesis of leukemia. We demonstrated that ZNF268 mRNA was repressed in HTLV-1-infected cells. We also showed that stable and transient expression of HTLV-1 Tax led to repression of ZNF268. In addition, by using reporter constructs that bear the human ZNF268 promoter and its mutants, we showed that Tax repressed ZNF268 promoter in a process dependent on a functional cAMP-responsive element. By using Tax, cAMP-responsive element-binding protein (CREB)-1, CREB-2, and their mutants, we further showed that Tax repressed ZNF268 through the CREB/activating transcription factor pathway. Electrophoretic mobility shift assays and chromatin immunoprecipitation demonstrated the formation of the complex of Tax·CREB-1 directly at the cAMP-responsive element both in vitro and in vivo. These findings suggest a role for ZNF268 in aberrant T-cell proliferation observed in HTLV-1-associated diseases.</description><subject>Activating Transcription Factor 1 - metabolism</subject><subject>Base Sequence</subject><subject>Cyclic AMP - metabolism</subject><subject>Cyclic AMP Response Element-Binding Protein - metabolism</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>Gene Expression Regulation</subject><subject>Gene Products, tax - physiology</subject><subject>Human T-lymphotropic virus 1</subject><subject>Human T-lymphotropic virus 1 - metabolism</subject><subject>Humans</subject><subject>Mechanisms of Signal Transduction</subject><subject>Models, Biological</subject><subject>Models, Genetic</subject><subject>Molecular Sequence Data</subject><subject>Mutation</subject><subject>Promoter Regions, Genetic</subject><subject>Protein Binding</subject><subject>Repressor Proteins - metabolism</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFks9v0zAUxyMEYmVw5Qg-IG7pbMeJnQtSNbUMqWMVdAhxsRzntfFo4mAn3fov8VfiLBWDA8KXJ7338Vff9yOKXhI8JZizs5tCTy85zhjNKMaPognBIomTlHx9HE0wpiTOaSpOomfe3-DwWE6eRidEJDxNBJtEPy_6WjVoHWvY7dAS-u9QG4W-GNd7tD60gAi6arRtne3ABFDdoU_QOvAePPr2cUEzgeZ39wljG9RVzvbbKkRAena5ikOhtY03e0DzHdTQdHFhmtI0W7QaNc9mujN71Q2ptVON18603SC2ULqzDq1UV92qw_PoyUbtPLw4xtPoejFfn1_Ey6v3H85ny1inlHQxbHiSshTnwIkmPFEl01wVpRAZhoIInG8041ByzLKyzLIyCdOgTDCd5YUo8uQ0ejfqtn1RQ6mDZad2snWmVu4grTLy70pjKrm1e0kZTWiKg8Dbo4CzP3rwnayNH-arGrC9l5xkXGCS_RekmIs0u1ecjqB21nsHm99uCJbDHchwB_LhDsKHV3_28IAfFx-ANyNQmW11axzIwlhdQS2pSEInkmQ0H2bxesQ2ykq1dcbL688UkwTjHFPBhh7ESEBYyd6Ak14baDSUQVR3srTmXyZ_Ae4g2ZI</recordid><startdate>20080613</startdate><enddate>20080613</enddate><creator>Wang, Di</creator><creator>Guo, Ming-Xiong</creator><creator>Hu, Hai-Ming</creator><creator>Zhao, Zhou-Zhou</creator><creator>Qiu, Hong-Ling</creator><creator>Shao, Huan-Jie</creator><creator>Zhu, Chen-Gang</creator><creator>Xue, Lu</creator><creator>Shi, Yun-Bo</creator><creator>Li, Wen-Xin</creator><general>Elsevier Inc</general><general>American Society for Biochemistry and Molecular Biology</general><scope>6I.</scope><scope>AAFTH</scope><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20080613</creationdate><title>Human T-cell Leukemia Virus Type 1 Oncoprotein Tax Represses ZNF268 Expression through the cAMP-responsive Element-binding Protein/Activating Transcription Factor Pathway</title><author>Wang, Di ; Guo, Ming-Xiong ; Hu, Hai-Ming ; Zhao, Zhou-Zhou ; Qiu, Hong-Ling ; Shao, Huan-Jie ; Zhu, Chen-Gang ; Xue, Lu ; Shi, Yun-Bo ; Li, Wen-Xin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c521t-ef7354509e71c173ad4c7abd8860eb1809fc47ed7046dd66d31832484c69b8b93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Activating Transcription Factor 1 - metabolism</topic><topic>Base Sequence</topic><topic>Cyclic AMP - metabolism</topic><topic>Cyclic AMP Response Element-Binding Protein - metabolism</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>Gene Expression Regulation</topic><topic>Gene Products, tax - physiology</topic><topic>Human T-lymphotropic virus 1</topic><topic>Human T-lymphotropic virus 1 - metabolism</topic><topic>Humans</topic><topic>Mechanisms of Signal Transduction</topic><topic>Models, Biological</topic><topic>Models, Genetic</topic><topic>Molecular Sequence Data</topic><topic>Mutation</topic><topic>Promoter Regions, Genetic</topic><topic>Protein Binding</topic><topic>Repressor Proteins - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Di</creatorcontrib><creatorcontrib>Guo, Ming-Xiong</creatorcontrib><creatorcontrib>Hu, Hai-Ming</creatorcontrib><creatorcontrib>Zhao, Zhou-Zhou</creatorcontrib><creatorcontrib>Qiu, Hong-Ling</creatorcontrib><creatorcontrib>Shao, Huan-Jie</creatorcontrib><creatorcontrib>Zhu, Chen-Gang</creatorcontrib><creatorcontrib>Xue, Lu</creatorcontrib><creatorcontrib>Shi, Yun-Bo</creatorcontrib><creatorcontrib>Li, Wen-Xin</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Di</au><au>Guo, Ming-Xiong</au><au>Hu, Hai-Ming</au><au>Zhao, Zhou-Zhou</au><au>Qiu, Hong-Ling</au><au>Shao, Huan-Jie</au><au>Zhu, Chen-Gang</au><au>Xue, Lu</au><au>Shi, Yun-Bo</au><au>Li, Wen-Xin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human T-cell Leukemia Virus Type 1 Oncoprotein Tax Represses ZNF268 Expression through the cAMP-responsive Element-binding Protein/Activating Transcription Factor Pathway</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>2008-06-13</date><risdate>2008</risdate><volume>283</volume><issue>24</issue><spage>16299</spage><epage>16308</epage><pages>16299-16308</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><abstract>Expression of the human T-cell leukemia virus type 1 (HTLV-1) oncoprotein Tax is correlated with cellular transformation, contributing to the development of adult T-cell leukemia. In this study, we investigated the role of Tax in the regulation of the ZNF268 gene, which plays a role in the differentiation of blood cells and the pathogenesis of leukemia. We demonstrated that ZNF268 mRNA was repressed in HTLV-1-infected cells. We also showed that stable and transient expression of HTLV-1 Tax led to repression of ZNF268. In addition, by using reporter constructs that bear the human ZNF268 promoter and its mutants, we showed that Tax repressed ZNF268 promoter in a process dependent on a functional cAMP-responsive element. By using Tax, cAMP-responsive element-binding protein (CREB)-1, CREB-2, and their mutants, we further showed that Tax repressed ZNF268 through the CREB/activating transcription factor pathway. Electrophoretic mobility shift assays and chromatin immunoprecipitation demonstrated the formation of the complex of Tax·CREB-1 directly at the cAMP-responsive element both in vitro and in vivo. These findings suggest a role for ZNF268 in aberrant T-cell proliferation observed in HTLV-1-associated diseases.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>18375384</pmid><doi>10.1074/jbc.M706426200</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Activating Transcription Factor 1 - metabolism Base Sequence Cyclic AMP - metabolism Cyclic AMP Response Element-Binding Protein - metabolism DNA-Binding Proteins - metabolism Gene Expression Regulation Gene Products, tax - physiology Human T-lymphotropic virus 1 Human T-lymphotropic virus 1 - metabolism Humans Mechanisms of Signal Transduction Models, Biological Models, Genetic Molecular Sequence Data Mutation Promoter Regions, Genetic Protein Binding Repressor Proteins - metabolism |
| title | Human T-cell Leukemia Virus Type 1 Oncoprotein Tax Represses ZNF268 Expression through the cAMP-responsive Element-binding Protein/Activating Transcription Factor Pathway |
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