Differential stabilization of reaction intermediates: specificity checkpoints for M.EcoRI revealed by transient fluorescence and fluorescence lifetime studies

M.EcoRI, a bacterial sequence-specific S-adenosyl-l-methionine-dependent DNA methyltransferase, relies on a complex conformational mechanism to achieve its remarkable specificity, including DNA bending, base flipping and intercalation into the DNA. Using transient fluorescence and fluorescence lifet...

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Veröffentlicht in:	Nucleic acids research 2008-05, Vol.36 (9), p.2917-2925
Hauptverfasser:	Youngblood, Ben, Bonnist, Eleanor, Dryden, David T.F, Jones, Anita C, Reich, Norbert O
Format:	Artikel
Sprache:	eng
Schlagworte:	DNA - chemistry DNA - metabolism Fluorescence Resonance Energy Transfer Kinetics Mutation Nucleic Acid Enzymes Site-Specific DNA-Methyltransferase (Adenine-Specific) - genetics Site-Specific DNA-Methyltransferase (Adenine-Specific) - metabolism Substrate Specificity
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