A sub-proteome of Arabidopsis thaliana mature stems trapped on Concanavalin A is enriched in cell wall glycoside hydrolases

N-glycosylated proteins were isolated from Arabidopsis thaliana mature stems using affinity chromatography on Concanavalin A Sepharose, separated by 2D-electrophoresis and identified using nanoHPLC-MS/MS and MALDI-TOF MS. 102 glycoproteins were identified. 94% of these proteins were predicted by bio...

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Veröffentlicht in:	Journal of experimental botany 2007-01, Vol.58 (10), p.2503-2512
Hauptverfasser:	Minic, Zoran, Jamet, Elisabeth, Négroni, Luc, Arsene der Garabedian, P, Zivy, Michel, Jouanin, Lise
Format:	Artikel
Sprache:	eng
Schlagworte:	Arabidopsis - enzymology Arabidopsis - growth & development Arabidopsis Proteins - classification Arabidopsis Proteins - metabolism Arabidopsis Proteins - physiology Arabidopsis thaliana Biochemistry, Molecular Biology Biological and medical sciences Cell biochemistry Cell physiology cell wall Cell Wall - enzymology Cell walls Chromatography, Affinity - methods Chromatography, High Pressure Liquid Computational Biology Concanavalin A Concanavalin A - chemistry Enzymes Fundamental and applied biological sciences. Psychology Genomics glycoside hydrolase Glycoside Hydrolases - metabolism Glycoside Hydrolases - physiology Glycosides Glycosylation Life Sciences Oxidases Plant biochemistry Plant cells Plant physiology and development plant stem Plant Stems - enzymology Plant Stems - growth & development Plants Polysaccharides Proteins Proteome Proteomics Research Papers Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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