Curcuma longa extract as a histological dye for collagen fibres and red blood cells
Crude ethanolic extract and column chromatographic fractions of the Allepey cultivar of Curcuma longa Roxb, commonly called turmeric (tumeric) in commerce, were used as a stain for tissue sections. Staining was carried out under basic, acidic and neutral media conditions. Inorganic and organic disso...
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| Veröffentlicht in: | Journal of anatomy 2007-05, Vol.210 (5), p.600-603 |
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| container_title | Journal of anatomy |
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| creator | Avwioro, O. G. Onwuka, S. K. Moody, J. O. Agbedahunsi, J. M. Oduola, T. Ekpo, O. E. Oladele, A. A. |
| description | Crude ethanolic extract and column chromatographic fractions of the Allepey cultivar of Curcuma longa Roxb, commonly called turmeric (tumeric) in commerce, were used as a stain for tissue sections. Staining was carried out under basic, acidic and neutral media conditions. Inorganic and organic dissolution solvents were used. The stain was used as a counterstain after alum and iron haematoxylins. C. longa stained collagen fibres, cytoplasm, red blood cells and muscle cells yellow. It also stained in a fashion similar to eosin, except for its intense yellow colour. Preliminary phytochemical evaluation of the active column fraction revealed that it contained flavonoids, free anthraquinone and deoxy sugar. A cheap, natural dye can thus be obtained from C. longa. |
| doi_str_mv | 10.1111/j.1469-7580.2007.00721.x |
| format | Article |
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A cheap, natural dye can thus be obtained from C. longa.</description><identifier>ISSN: 0021-8782</identifier><identifier>EISSN: 1469-7580</identifier><identifier>DOI: 10.1111/j.1469-7580.2007.00721.x</identifier><identifier>PMID: 17451535</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Coloring Agents ; Curcuma ; Curcuma longa ; Cytoplasm - ultrastructure ; Erythrocytes - cytology ; Fibrillar Collagens - ultrastructure ; flavonoid and stain ; Humans ; Methods ; Muscle Cells - cytology ; Plant Extracts ; rhizome ; Staining and Labeling - methods ; turmeric (tumeric) ; Zingiberaceae</subject><ispartof>Journal of anatomy, 2007-05, Vol.210 (5), p.600-603</ispartof><rights>2007 The Authors Journal compilation © 2007 Anatomical Society of Great Britain and Ireland 2007</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4711-81650c3497f8f25dd479f765d3779c69cc6c0962cc89a4b4e61e70279a9e09f93</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2375739/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2375739/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,1416,1432,27923,27924,45573,45574,46408,46832,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17451535$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Avwioro, O. G.</creatorcontrib><creatorcontrib>Onwuka, S. K.</creatorcontrib><creatorcontrib>Moody, J. O.</creatorcontrib><creatorcontrib>Agbedahunsi, J. M.</creatorcontrib><creatorcontrib>Oduola, T.</creatorcontrib><creatorcontrib>Ekpo, O. E.</creatorcontrib><creatorcontrib>Oladele, A. A.</creatorcontrib><title>Curcuma longa extract as a histological dye for collagen fibres and red blood cells</title><title>Journal of anatomy</title><addtitle>J Anat</addtitle><description>Crude ethanolic extract and column chromatographic fractions of the Allepey cultivar of Curcuma longa Roxb, commonly called turmeric (tumeric) in commerce, were used as a stain for tissue sections. Staining was carried out under basic, acidic and neutral media conditions. Inorganic and organic dissolution solvents were used. The stain was used as a counterstain after alum and iron haematoxylins. C. longa stained collagen fibres, cytoplasm, red blood cells and muscle cells yellow. It also stained in a fashion similar to eosin, except for its intense yellow colour. Preliminary phytochemical evaluation of the active column fraction revealed that it contained flavonoids, free anthraquinone and deoxy sugar. A cheap, natural dye can thus be obtained from C. longa.</description><subject>Coloring Agents</subject><subject>Curcuma</subject><subject>Curcuma longa</subject><subject>Cytoplasm - ultrastructure</subject><subject>Erythrocytes - cytology</subject><subject>Fibrillar Collagens - ultrastructure</subject><subject>flavonoid and stain</subject><subject>Humans</subject><subject>Methods</subject><subject>Muscle Cells - cytology</subject><subject>Plant Extracts</subject><subject>rhizome</subject><subject>Staining and Labeling - methods</subject><subject>turmeric (tumeric)</subject><subject>Zingiberaceae</subject><issn>0021-8782</issn><issn>1469-7580</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkV9PwyAUxYnRuDn9CoYn31ahQCmJMVkW_2bJHtRnQindurAyYdXt20vdMvVNEgLJ_d3D4R4AIEYJjut6kWCaiSFnOUpShHgSd4qTzRHoHwrHoI9Qioc5z9MeOAthgRAmSNBT0MOcMswI64OXcet1u1TQumamoNmsvdJrqAJUcF6HtbNuVmtlYbk1sHIeametmpkGVnXhTcSaEnpTwsI6V0JtrA3n4KRSNpiL_TkAb_d3r-PH4WT68DQeTYaachyN4YwhTajgVV6lrCwpFxXPWEk4FzoTWmcaiSzVOheKFtRk2HCUcqGEQaISZABud7qrtliaUpsmmrdy5eul8lvpVC3_Vpp6LmfuQ6aEM046gau9gHfvrQlruaxD9wXVGNcGyRHFJCMsgvkO1N6F4E11eAQj2SUiF7IbvOwGL7tE5HcichNbL3-b_GncRxCBmx3wWVuz_bewfJ6O4oV8AeY0mlw</recordid><startdate>200705</startdate><enddate>200705</enddate><creator>Avwioro, O. G.</creator><creator>Onwuka, S. K.</creator><creator>Moody, J. O.</creator><creator>Agbedahunsi, J. M.</creator><creator>Oduola, T.</creator><creator>Ekpo, O. E.</creator><creator>Oladele, A. A.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell Publishing Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>200705</creationdate><title>Curcuma longa extract as a histological dye for collagen fibres and red blood cells</title><author>Avwioro, O. G. ; Onwuka, S. K. ; Moody, J. O. ; Agbedahunsi, J. M. ; Oduola, T. ; Ekpo, O. E. ; Oladele, A. A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4711-81650c3497f8f25dd479f765d3779c69cc6c0962cc89a4b4e61e70279a9e09f93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Coloring Agents</topic><topic>Curcuma</topic><topic>Curcuma longa</topic><topic>Cytoplasm - ultrastructure</topic><topic>Erythrocytes - cytology</topic><topic>Fibrillar Collagens - ultrastructure</topic><topic>flavonoid and stain</topic><topic>Humans</topic><topic>Methods</topic><topic>Muscle Cells - cytology</topic><topic>Plant Extracts</topic><topic>rhizome</topic><topic>Staining and Labeling - methods</topic><topic>turmeric (tumeric)</topic><topic>Zingiberaceae</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Avwioro, O. G.</creatorcontrib><creatorcontrib>Onwuka, S. K.</creatorcontrib><creatorcontrib>Moody, J. 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A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Curcuma longa extract as a histological dye for collagen fibres and red blood cells</atitle><jtitle>Journal of anatomy</jtitle><addtitle>J Anat</addtitle><date>2007-05</date><risdate>2007</risdate><volume>210</volume><issue>5</issue><spage>600</spage><epage>603</epage><pages>600-603</pages><issn>0021-8782</issn><eissn>1469-7580</eissn><abstract>Crude ethanolic extract and column chromatographic fractions of the Allepey cultivar of Curcuma longa Roxb, commonly called turmeric (tumeric) in commerce, were used as a stain for tissue sections. Staining was carried out under basic, acidic and neutral media conditions. Inorganic and organic dissolution solvents were used. The stain was used as a counterstain after alum and iron haematoxylins. C. longa stained collagen fibres, cytoplasm, red blood cells and muscle cells yellow. 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| subjects | Coloring Agents Curcuma Curcuma longa Cytoplasm - ultrastructure Erythrocytes - cytology Fibrillar Collagens - ultrastructure flavonoid and stain Humans Methods Muscle Cells - cytology Plant Extracts rhizome Staining and Labeling - methods turmeric (tumeric) Zingiberaceae |
| title | Curcuma longa extract as a histological dye for collagen fibres and red blood cells |
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