Identification of gene clusters differentially expressed during the cellular injury responses (CIR) to cisplatin
The goal of this study was to identify changes in mRNA levels in tumour cells after a toxic exposure to cisplatin (IC 99 dose). Using suppression-subtractive hybridization (SSH) 2 cDNA libraries were created, an UP library (202 cDNA fragments) and a DOWN library (153 cDNA fragments). Using reversed...
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| Veröffentlicht in: | British journal of cancer 2001-10, Vol.85 (8), p.1206-1210 |
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| creator | Johnsson, A Byrne, P Bruin, R de Weiner, D Wong, J Los, G |
| description | The goal of this study was to identify changes in mRNA levels in tumour cells after a toxic exposure to cisplatin (IC
99
dose). Using suppression-subtractive hybridization (SSH) 2 cDNA libraries were created, an UP library (202 cDNA fragments) and a DOWN library (153 cDNA fragments). Using reversed Northern hybridization 16 and 30 fragments were truly differentially expressed in the UP and DOWN libraries, respectively. Most prominent in the UP library were the mitochondrial and injury response clusters and in the DOWN library the cytoskeletal, protein synthesis and signalling clusters. These distinct clusters potentially represent an expression profile of the cisplatin-induced cellular injury response. © 2001 Cancer Research Campaign
http://www.bjcancer.com |
| doi_str_mv | 10.1054/bjoc.2001.2080 |
| format | Article |
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99
dose). Using suppression-subtractive hybridization (SSH) 2 cDNA libraries were created, an UP library (202 cDNA fragments) and a DOWN library (153 cDNA fragments). Using reversed Northern hybridization 16 and 30 fragments were truly differentially expressed in the UP and DOWN libraries, respectively. Most prominent in the UP library were the mitochondrial and injury response clusters and in the DOWN library the cytoskeletal, protein synthesis and signalling clusters. These distinct clusters potentially represent an expression profile of the cisplatin-induced cellular injury response. © 2001 Cancer Research Campaign
http://www.bjcancer.com</description><identifier>ISSN: 0007-0920</identifier><identifier>EISSN: 1532-1827</identifier><identifier>DOI: 10.1054/bjoc.2001.2080</identifier><identifier>PMID: 11710836</identifier><identifier>CODEN: BJCAAI</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Antineoplastic agents ; Antineoplastic Agents - pharmacology ; Apoptosis ; Biological and medical sciences ; Biomedical and Life Sciences ; Biomedicine ; Blotting, Northern ; Cancer Research ; Cell Survival - drug effects ; Cisplatin - pharmacology ; DNA, Complementary - analysis ; DNA-Binding Proteins - genetics ; Drug Resistance ; Epidemiology ; Gene expression ; Gene Expression Profiling ; General aspects ; HSP90 Heat-Shock Proteins - genetics ; Humans ; Hybridization ; Laboratories ; Medical research ; Medical sciences ; Molecular Medicine ; Multigene Family ; Nuclear Proteins ; Oncology ; Pharmacology. Drug treatments ; Proto-Oncogene Proteins - genetics ; Proto-Oncogene Proteins c-mdm2 ; Short Communication ; Tumor Cells, Cultured</subject><ispartof>British journal of cancer, 2001-10, Vol.85 (8), p.1206-1210</ispartof><rights>The Author(s) 2001</rights><rights>2002 INIST-CNRS</rights><rights>Copyright 2001 Cancer Research Campaign http://www.bjcancer.com.</rights><rights>Copyright Nature Publishing Group Oct 2001</rights><rights>Copyright © 2001 Cancer Research Campaign 2001 Cancer Research Campaign</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c515t-68032f343077c921d59613ada883b0ac029227a716e3dd6e30456ca49c2ec98f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2375161/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2375161/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,41464,42533,51294,53766,53768</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14133296$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11710836$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Johnsson, A</creatorcontrib><creatorcontrib>Byrne, P</creatorcontrib><creatorcontrib>Bruin, R de</creatorcontrib><creatorcontrib>Weiner, D</creatorcontrib><creatorcontrib>Wong, J</creatorcontrib><creatorcontrib>Los, G</creatorcontrib><title>Identification of gene clusters differentially expressed during the cellular injury responses (CIR) to cisplatin</title><title>British journal of cancer</title><addtitle>Br J Cancer</addtitle><addtitle>Br J Cancer</addtitle><description>The goal of this study was to identify changes in mRNA levels in tumour cells after a toxic exposure to cisplatin (IC
99
dose). Using suppression-subtractive hybridization (SSH) 2 cDNA libraries were created, an UP library (202 cDNA fragments) and a DOWN library (153 cDNA fragments). Using reversed Northern hybridization 16 and 30 fragments were truly differentially expressed in the UP and DOWN libraries, respectively. Most prominent in the UP library were the mitochondrial and injury response clusters and in the DOWN library the cytoskeletal, protein synthesis and signalling clusters. These distinct clusters potentially represent an expression profile of the cisplatin-induced cellular injury response. © 2001 Cancer Research Campaign
http://www.bjcancer.com</description><subject>Antineoplastic agents</subject><subject>Antineoplastic Agents - pharmacology</subject><subject>Apoptosis</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Blotting, Northern</subject><subject>Cancer Research</subject><subject>Cell Survival - drug effects</subject><subject>Cisplatin - pharmacology</subject><subject>DNA, Complementary - analysis</subject><subject>DNA-Binding Proteins - genetics</subject><subject>Drug Resistance</subject><subject>Epidemiology</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>General aspects</subject><subject>HSP90 Heat-Shock Proteins - genetics</subject><subject>Humans</subject><subject>Hybridization</subject><subject>Laboratories</subject><subject>Medical research</subject><subject>Medical sciences</subject><subject>Molecular Medicine</subject><subject>Multigene Family</subject><subject>Nuclear Proteins</subject><subject>Oncology</subject><subject>Pharmacology. Drug treatments</subject><subject>Proto-Oncogene Proteins - genetics</subject><subject>Proto-Oncogene Proteins c-mdm2</subject><subject>Short Communication</subject><subject>Tumor Cells, Cultured</subject><issn>0007-0920</issn><issn>1532-1827</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp1kd2LEzEUxYMobq2--ihBkNWH6d4kk0nmZUGKH4UFQfQ5pJlMNyVNxmRmsf-9GVqsCr7cEO4v99yTg9BLAisCvL7Z7qNZUQBSioRHaEE4oxWRVDxGCwAQFbQUrtCznPfl2oIUT9EVIYKAZM0CDZvOhtH1zujRxYBjj3c2WGz8lEebMu5c39s0M9r7I7Y_h2Rzth3upuTCDo_3BbbeT14n7MJ-SkdciCGGbDN-u958fYfHiI3Lgy8S4Tl60muf7YvzuUTfP374tv5c3X35tFm_v6sMJ3ysGgmM9qxmIIRpKel42xCmOy0l24I2QFtKhRaksazrSoGaN0bXraHWtLJnS3R7mjtM24PtTHGQtFdDcgedjipqp_7uBHevdvFBUSY4KVpLdH0ekOKPyeZRHVyenepg45RVy2suail5IV__Q-7jlEJxV4YBUMabpkCrE2RSzDnZ_vcqBNQcpZqjVHOUao6yPHj1p4ELfs6uAG_OgM5G-z7pUH75wtWEMdrO3M2Jy8OcmE2X9f4j_QuQZLgv</recordid><startdate>20011019</startdate><enddate>20011019</enddate><creator>Johnsson, A</creator><creator>Byrne, P</creator><creator>Bruin, R de</creator><creator>Weiner, D</creator><creator>Wong, J</creator><creator>Los, G</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AN0</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PJZUB</scope><scope>PKEHL</scope><scope>PPXIY</scope><scope>PQEST</scope><scope>PQGLB</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>5PM</scope></search><sort><creationdate>20011019</creationdate><title>Identification of gene clusters differentially expressed during the cellular injury responses (CIR) to cisplatin</title><author>Johnsson, A ; Byrne, P ; Bruin, R de ; Weiner, D ; Wong, J ; Los, G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c515t-68032f343077c921d59613ada883b0ac029227a716e3dd6e30456ca49c2ec98f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Antineoplastic agents</topic><topic>Antineoplastic Agents - pharmacology</topic><topic>Apoptosis</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Blotting, Northern</topic><topic>Cancer Research</topic><topic>Cell Survival - drug effects</topic><topic>Cisplatin - pharmacology</topic><topic>DNA, Complementary - analysis</topic><topic>DNA-Binding Proteins - genetics</topic><topic>Drug Resistance</topic><topic>Epidemiology</topic><topic>Gene expression</topic><topic>Gene Expression Profiling</topic><topic>General aspects</topic><topic>HSP90 Heat-Shock Proteins - genetics</topic><topic>Humans</topic><topic>Hybridization</topic><topic>Laboratories</topic><topic>Medical research</topic><topic>Medical sciences</topic><topic>Molecular Medicine</topic><topic>Multigene Family</topic><topic>Nuclear Proteins</topic><topic>Oncology</topic><topic>Pharmacology. Drug treatments</topic><topic>Proto-Oncogene Proteins - genetics</topic><topic>Proto-Oncogene Proteins c-mdm2</topic><topic>Short Communication</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Johnsson, A</creatorcontrib><creatorcontrib>Byrne, P</creatorcontrib><creatorcontrib>Bruin, R de</creatorcontrib><creatorcontrib>Weiner, D</creatorcontrib><creatorcontrib>Wong, J</creatorcontrib><creatorcontrib>Los, G</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>British Nursing Database</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest Central (New)</collection><collection>ProQuest One Academic (New)</collection><collection>ProQuest Health & Medical Research Collection</collection><collection>ProQuest One Academic Middle East (New)</collection><collection>ProQuest One Health & Nursing</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Applied & Life Sciences</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>British journal of cancer</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Johnsson, A</au><au>Byrne, P</au><au>Bruin, R de</au><au>Weiner, D</au><au>Wong, J</au><au>Los, G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of gene clusters differentially expressed during the cellular injury responses (CIR) to cisplatin</atitle><jtitle>British journal of cancer</jtitle><stitle>Br J Cancer</stitle><addtitle>Br J Cancer</addtitle><date>2001-10-19</date><risdate>2001</risdate><volume>85</volume><issue>8</issue><spage>1206</spage><epage>1210</epage><pages>1206-1210</pages><issn>0007-0920</issn><eissn>1532-1827</eissn><coden>BJCAAI</coden><abstract>The goal of this study was to identify changes in mRNA levels in tumour cells after a toxic exposure to cisplatin (IC
99
dose). Using suppression-subtractive hybridization (SSH) 2 cDNA libraries were created, an UP library (202 cDNA fragments) and a DOWN library (153 cDNA fragments). Using reversed Northern hybridization 16 and 30 fragments were truly differentially expressed in the UP and DOWN libraries, respectively. Most prominent in the UP library were the mitochondrial and injury response clusters and in the DOWN library the cytoskeletal, protein synthesis and signalling clusters. These distinct clusters potentially represent an expression profile of the cisplatin-induced cellular injury response. © 2001 Cancer Research Campaign
http://www.bjcancer.com</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>11710836</pmid><doi>10.1054/bjoc.2001.2080</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Springer Nature - Complete Springer Journals; Nature Journals Online; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Antineoplastic agents Antineoplastic Agents - pharmacology Apoptosis Biological and medical sciences Biomedical and Life Sciences Biomedicine Blotting, Northern Cancer Research Cell Survival - drug effects Cisplatin - pharmacology DNA, Complementary - analysis DNA-Binding Proteins - genetics Drug Resistance Epidemiology Gene expression Gene Expression Profiling General aspects HSP90 Heat-Shock Proteins - genetics Humans Hybridization Laboratories Medical research Medical sciences Molecular Medicine Multigene Family Nuclear Proteins Oncology Pharmacology. Drug treatments Proto-Oncogene Proteins - genetics Proto-Oncogene Proteins c-mdm2 Short Communication Tumor Cells, Cultured |
| title | Identification of gene clusters differentially expressed during the cellular injury responses (CIR) to cisplatin |
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