The N‐terminal portion of mature aldehyde dehydrogenase affects protein folding and assembly

Human liver cytosolic (ALDH1) and mitochondrial (ALDH2) aldehyde dehydrogenases are both encoded in the nucleus and synthesized in the cytosol. ALDH1 must fold in the cytosol, but ALDH2 is first synthesized as a precursor and must remain unfolded during import into mitochondria. The two mature forms...

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Veröffentlicht in:	Protein science 2001-08, Vol.10 (8), p.1490-1497
Hauptverfasser:	Zhou, Jianzhong, Weiner, Henry
Format:	Artikel
Sprache:	eng
Schlagworte:	Aldehyde dehydrogenase Aldehyde Dehydrogenase - chemistry Aldehyde Dehydrogenase - metabolism ALDH, aldehyde dehydrogenase ALDH1, cytosolic aldehyde dehydrogenase ALDH2, mitochondrial mature aldehyde dehydrogenase Amino Acid Sequence Cell-Free System Centrifugation, Density Gradient chimeric proteins Cytosol - enzymology C‐ALDH2, chimeric protein of ALDH2 in which the first 21 amino acid residues of the mature protein were replaced by the corresponding residues from ALDH1 DTT, dithiotreitol Enzyme Stability folding HEPES, N‐(2‐hydroxyethyl) piperazine‐N′‐(2‐ethanesulfonic acid) HEPPS, N‐2‐hydroxyethylpiperazine‐N′‐3‐propanesulfonic acid Humans import into mitochondria Isoenzymes - chemistry Isoenzymes - metabolism Liver - enzymology Mitochondria - enzymology Molecular Sequence Data M‐ALDH1, chimeric protein of ALDH1 in which the first 21 amino acid residues of the mature protein were replaced by the corresponding residues from ALDH2 N‐terminal portion PAGE, polyacrylamide gel electrophoresis pALDH1, the presequence of pALDH2 fused to the ALDH1 pALDH2, mitochondrial aldehyde dehydrogenase precursor PCR, polymerase chain reaction pC‐ALDH2, the presequence of pALDH2 fused to C‐ALDH2 pM‐ALDH1, the presequence of pALDH2 fused to M‐ALDH1 Protein Denaturation Protein Folding Protein Precursors - metabolism Protein Structure, Tertiary Protein Transport Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism Trypsin - pharmacology Urea - pharmacology Yeasts - physiology
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