Semi-automated microseeding of nanolitre crystallization experiments

A simple semi‐automated microseeding procedure for nanolitre crystallization experiments is described. Firstly, a microseed stock solution is made from microcrystals using a Teflon bead. A dilution series of this microseed stock is then prepared and dispensed as 100 nl droplets into 96‐well crystall...

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Veröffentlicht in:	Acta crystallographica. Section F, Structural biology and crystallization communications Structural biology and crystallization communications, 2008-01, Vol.64 (1), p.14-18
Hauptverfasser:	Walter, Thomas S., Mancini, Erika J., Kadlec, Jan, Graham, Stephen C., Assenberg, René, Ren, Jingshan, Sainsbury, Sarah, Owens, Raymond J., Stuart, David I., Grimes, Jonathan M., Harlos, Karl
Format:	Artikel
Sprache:	eng
Schlagworte:	additives Automation Bacterial Proteins - chemistry crystal optimization crystallization Crystallization Communications Crystallography, X-Ray - methods Escherichia coli Proteins - chemistry Genomics microseeding Models, Molecular Molecular Conformation Nanotechnology - methods Proteomics Viral Proteins - chemistry
Online-Zugang:	Volltext
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