Semi-automated microseeding of nanolitre crystallization experiments
A simple semi‐automated microseeding procedure for nanolitre crystallization experiments is described. Firstly, a microseed stock solution is made from microcrystals using a Teflon bead. A dilution series of this microseed stock is then prepared and dispensed as 100 nl droplets into 96‐well crystall...
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| Veröffentlicht in: | Acta crystallographica. Section F, Structural biology and crystallization communications Structural biology and crystallization communications, 2008-01, Vol.64 (1), p.14-18 |
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| container_title | Acta crystallographica. Section F, Structural biology and crystallization communications |
| container_volume | 64 |
| creator | Walter, Thomas S. Mancini, Erika J. Kadlec, Jan Graham, Stephen C. Assenberg, René Ren, Jingshan Sainsbury, Sarah Owens, Raymond J. Stuart, David I. Grimes, Jonathan M. Harlos, Karl |
| description | A simple semi‐automated microseeding procedure for nanolitre crystallization experiments is described. Firstly, a microseed stock solution is made from microcrystals using a Teflon bead. A dilution series of this microseed stock is then prepared and dispensed as 100 nl droplets into 96‐well crystallization plates, facilitating the incorporation of seeding into high‐throughput crystallization pipelines. This basic microseeding procedure has been modified to include additive‐screening and cross‐seeding methods. Five examples in which these techniques have been used successfully are described. |
| doi_str_mv | 10.1107/S1744309107057260 |
| format | Article |
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Firstly, a microseed stock solution is made from microcrystals using a Teflon bead. A dilution series of this microseed stock is then prepared and dispensed as 100 nl droplets into 96‐well crystallization plates, facilitating the incorporation of seeding into high‐throughput crystallization pipelines. This basic microseeding procedure has been modified to include additive‐screening and cross‐seeding methods. Five examples in which these techniques have been used successfully are described.</description><identifier>ISSN: 1744-3091</identifier><identifier>EISSN: 1744-3091</identifier><identifier>DOI: 10.1107/S1744309107057260</identifier><identifier>PMID: 18097093</identifier><language>eng</language><publisher>5 Abbey Square, Chester, Cheshire CH1 2HU, England: Blackwell Publishing Ltd</publisher><subject>additives ; Automation ; Bacterial Proteins - chemistry ; crystal optimization ; crystallization ; Crystallization Communications ; Crystallography, X-Ray - methods ; Escherichia coli Proteins - chemistry ; Genomics ; microseeding ; Models, Molecular ; Molecular Conformation ; Nanotechnology - methods ; Proteomics ; Viral Proteins - chemistry</subject><ispartof>Acta crystallographica. 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Five examples in which these techniques have been used successfully are described.</description><subject>additives</subject><subject>Automation</subject><subject>Bacterial Proteins - chemistry</subject><subject>crystal optimization</subject><subject>crystallization</subject><subject>Crystallization Communications</subject><subject>Crystallography, X-Ray - methods</subject><subject>Escherichia coli Proteins - chemistry</subject><subject>Genomics</subject><subject>microseeding</subject><subject>Models, Molecular</subject><subject>Molecular Conformation</subject><subject>Nanotechnology - methods</subject><subject>Proteomics</subject><subject>Viral Proteins - chemistry</subject><issn>1744-3091</issn><issn>1744-3091</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEFP3DAQhS1UBJTyA7hUOfWW1mPHsX2pRCkLSKtyoBXqyXLiMXVJ4q2dpWx_fbPaFQX1wGlGM-97M3qEHAN9D0Dlh2uQVcWpnnoqJKvpDjlYj8r17NWTfp-8zvknpZzrWu2RfVBUS6r5Afl8jX0o7XKMvR3RFX1oU8yILgy3RfTFYIfYhTFh0aZVHm3XhT92DHEo8GGBKfQ4jPkN2fW2y3i0rYfk2-zs6-lFOb86vzw9mZdtpYQuFVS-YVZ7rnTdeBA1q5Rn0EinmGutpk6CQkFbdMJxyxU44L7yTFgnGuSH5OPGd7FsenTtdDvZziymN2xamWiDeb4Zwg9zG-8N45JrTSeDd1uDFH8tMY-mD7nFrrMDxmU2koKoGIdJCBvhOo2c0D8eAWrW2Zv_sp-Yt0-_-0dsw54EeiP4HTpcvexoTr7P2KcbwUBPbLlhQx7x4ZG16c7Ukkthbr6cmxlVag4X2lT8L5XnoHI</recordid><startdate>200801</startdate><enddate>200801</enddate><creator>Walter, Thomas S.</creator><creator>Mancini, Erika J.</creator><creator>Kadlec, Jan</creator><creator>Graham, Stephen C.</creator><creator>Assenberg, René</creator><creator>Ren, Jingshan</creator><creator>Sainsbury, Sarah</creator><creator>Owens, Raymond J.</creator><creator>Stuart, David I.</creator><creator>Grimes, Jonathan M.</creator><creator>Harlos, Karl</creator><general>Blackwell Publishing Ltd</general><general>International Union of Crystallography</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>200801</creationdate><title>Semi-automated microseeding of nanolitre crystallization experiments</title><author>Walter, Thomas S. ; Mancini, Erika J. ; Kadlec, Jan ; Graham, Stephen C. ; Assenberg, René ; Ren, Jingshan ; Sainsbury, Sarah ; Owens, Raymond J. ; Stuart, David I. ; Grimes, Jonathan M. ; Harlos, Karl</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4859-814fb2a9f3896bf156248f21b7d82dca90d718e50ced5d3a381d13f4f25ad5be3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>additives</topic><topic>Automation</topic><topic>Bacterial Proteins - chemistry</topic><topic>crystal optimization</topic><topic>crystallization</topic><topic>Crystallization Communications</topic><topic>Crystallography, X-Ray - methods</topic><topic>Escherichia coli Proteins - chemistry</topic><topic>Genomics</topic><topic>microseeding</topic><topic>Models, Molecular</topic><topic>Molecular Conformation</topic><topic>Nanotechnology - methods</topic><topic>Proteomics</topic><topic>Viral Proteins - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Walter, Thomas S.</creatorcontrib><creatorcontrib>Mancini, Erika J.</creatorcontrib><creatorcontrib>Kadlec, Jan</creatorcontrib><creatorcontrib>Graham, Stephen C.</creatorcontrib><creatorcontrib>Assenberg, René</creatorcontrib><creatorcontrib>Ren, Jingshan</creatorcontrib><creatorcontrib>Sainsbury, Sarah</creatorcontrib><creatorcontrib>Owens, Raymond J.</creatorcontrib><creatorcontrib>Stuart, David I.</creatorcontrib><creatorcontrib>Grimes, Jonathan M.</creatorcontrib><creatorcontrib>Harlos, Karl</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Acta crystallographica. 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F</addtitle><date>2008-01</date><risdate>2008</risdate><volume>64</volume><issue>1</issue><spage>14</spage><epage>18</epage><pages>14-18</pages><issn>1744-3091</issn><eissn>1744-3091</eissn><abstract>A simple semi‐automated microseeding procedure for nanolitre crystallization experiments is described. Firstly, a microseed stock solution is made from microcrystals using a Teflon bead. A dilution series of this microseed stock is then prepared and dispensed as 100 nl droplets into 96‐well crystallization plates, facilitating the incorporation of seeding into high‐throughput crystallization pipelines. This basic microseeding procedure has been modified to include additive‐screening and cross‐seeding methods. Five examples in which these techniques have been used successfully are described.</abstract><cop>5 Abbey Square, Chester, Cheshire CH1 2HU, England</cop><pub>Blackwell Publishing Ltd</pub><pmid>18097093</pmid><doi>10.1107/S1744309107057260</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | additives Automation Bacterial Proteins - chemistry crystal optimization crystallization Crystallization Communications Crystallography, X-Ray - methods Escherichia coli Proteins - chemistry Genomics microseeding Models, Molecular Molecular Conformation Nanotechnology - methods Proteomics Viral Proteins - chemistry |
| title | Semi-automated microseeding of nanolitre crystallization experiments |
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