The kinetics of G‐CSF folding

The folding kinetics of G‐CSF were determined by trp‐fluorescence and far‐UV circular dichroism. Folding and unfolding was achieved by rapid dilution and mixing of the denaturant, GdnHCl. G‐CSF is a four‐helical bundle protein with two long loops between the first and second helices and between the...

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Veröffentlicht in:	Protein science 2002-10, Vol.11 (10), p.2504-2511
1. Verfasser:	Brems, David N.
Format:	Artikel
Sprache:	eng
Schlagworte:	Circular Dichroism Fluorescence Folding Granulocyte Colony-Stimulating Factor - metabolism G‐CSF Humans Hydrogen-Ion Concentration intermediates Kinetics Protein Denaturation - physiology Protein Folding stopped‐flow kinetics Tryptophan - analogs & derivatives Tryptophan - metabolism
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