Engineered lentivector targeting of dendritic cells for in vivo immunization
We report a method of inducing antigen production in dendritic cells by in vivo targeting with lentiviral vectors that specifically bind to the dendritic cell–surface protein DC-SIGN. To target dendritic cells, we enveloped the lentivector with a viral glycoprotein from Sindbis virus engineered to b...
Gespeichert in:
| Veröffentlicht in: | Nature biotechnology 2008-03, Vol.26 (3), p.326-334 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 334 |
|---|---|
| container_issue | 3 |
| container_start_page | 326 |
| container_title | Nature biotechnology |
| container_volume | 26 |
| creator | Yang, Lili Yang, Haiguang Rideout, Kendra Cho, Taehoon Joo, Kye il Ziegler, Leslie Elliot, Abigail Walls, Anthony Yu, Dongzi Baltimore, David Wang, Pin |
| description | We report a method of inducing antigen production in dendritic cells by
in vivo
targeting with lentiviral vectors that specifically bind to the dendritic cell–surface protein DC-SIGN. To target dendritic cells, we enveloped the lentivector with a viral glycoprotein from Sindbis virus engineered to be DC-SIGN–specific.
In vitro
, this lentivector specifically transduced dendritic cells and induced dendritic cell maturation. A high frequency (up to 12%) of ovalbumin (OVA)-specific CD8
+
T cells and a significant antibody response were observed 2 weeks after injection of a targeted lentiviral vector encoding an OVA transgene into naive mice. This approach also protected against the growth of OVA-expressing E.G7 tumors and induced regression of established tumors. Thus, lentiviral vectors targeting dendritic cells provide a simple method of producing effective immunity and may provide an alternative route for immunization with protein antigens. |
| doi_str_mv | 10.1038/nbt1390 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2366162</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A198474281</galeid><sourcerecordid>A198474281</sourcerecordid><originalsourceid>FETCH-LOGICAL-c661t-5237ca82f074cd8ab9ea1a500b3a88bf2abd93f2bdab75dc8df857ed49a63c163</originalsourceid><addsrcrecordid>eNqN0klv1DAUB_AIgegC4hugCIntMMVL4tgXpKoqUGmkSmxX68VLcJXYre2MKJ8el4laphdIDonin19e_nlV9QyjI4wof-f7jKlAD6p93DZshZlgD8s94t0K4ZbtVQcpXSCEWMPY42oPcyI61LL9an3qB-eNiUbXo_HZbYzKIdYZ4mCy80MdbK2N19Flp2plxjHVtgDn643bhNpN0-zdL8gu-CfVIwtjMk-X62H17cPp15NPq_X5x7OT4_VKMYbzqiW0U8CJRV2jNIdeGMDQItRT4Ly3BHotqCW9hr5rteLa8rYzuhHAqMKMHlbvt3Uv534yWpW-I4zyMroJ4rUM4OTuinc_5BA2ktDSACOlwKulQAxXs0lZTi7dfBt4E-Yku5Ip5Yj-ExLEBWk5LvDFPXgR5uhLCpKUg1PRiYKOtmiA0UjnbSjdqXJqMzkVvLGuPD_GgjddQ_5UfbuzoZhsfuYB5pTk2ZfP_2_Pv-_a11urYkgpGnubHUbyZqDkMlBFPv876ju3TFABLxcAScFoI3jl0q0jqMFN-d_Fvdm6VJb8YOJdQPff-RvfRuAh</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>222283979</pqid></control><display><type>article</type><title>Engineered lentivector targeting of dendritic cells for in vivo immunization</title><source>MEDLINE</source><source>Nature</source><source>SpringerNature Complete Journals</source><creator>Yang, Lili ; Yang, Haiguang ; Rideout, Kendra ; Cho, Taehoon ; Joo, Kye il ; Ziegler, Leslie ; Elliot, Abigail ; Walls, Anthony ; Yu, Dongzi ; Baltimore, David ; Wang, Pin</creator><creatorcontrib>Yang, Lili ; Yang, Haiguang ; Rideout, Kendra ; Cho, Taehoon ; Joo, Kye il ; Ziegler, Leslie ; Elliot, Abigail ; Walls, Anthony ; Yu, Dongzi ; Baltimore, David ; Wang, Pin</creatorcontrib><description>We report a method of inducing antigen production in dendritic cells by
in vivo
targeting with lentiviral vectors that specifically bind to the dendritic cell–surface protein DC-SIGN. To target dendritic cells, we enveloped the lentivector with a viral glycoprotein from Sindbis virus engineered to be DC-SIGN–specific.
In vitro
, this lentivector specifically transduced dendritic cells and induced dendritic cell maturation. A high frequency (up to 12%) of ovalbumin (OVA)-specific CD8
+
T cells and a significant antibody response were observed 2 weeks after injection of a targeted lentiviral vector encoding an OVA transgene into naive mice. This approach also protected against the growth of OVA-expressing E.G7 tumors and induced regression of established tumors. Thus, lentiviral vectors targeting dendritic cells provide a simple method of producing effective immunity and may provide an alternative route for immunization with protein antigens.</description><identifier>ISSN: 1087-0156</identifier><identifier>EISSN: 1546-1696</identifier><identifier>DOI: 10.1038/nbt1390</identifier><identifier>PMID: 18297056</identifier><identifier>CODEN: NABIF9</identifier><language>eng</language><publisher>New York: Nature Publishing Group US</publisher><subject>Agriculture ; Animals ; B-Lymphocytes - cytology ; B-Lymphocytes - immunology ; Bioinformatics ; Biological and medical sciences ; Biomedical and Life Sciences ; Biomedical Engineering/Biotechnology ; Biomedicine ; Biotechnology ; Cancer Vaccines - immunology ; CD8-Positive T-Lymphocytes - cytology ; CD8-Positive T-Lymphocytes - immunology ; Cell Adhesion Molecules - metabolism ; Cell Line ; Cellular biology ; Dendritic cells ; Dendritic Cells - cytology ; Dendritic Cells - immunology ; Dendritic Cells - metabolism ; Female ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; Genetic aspects ; Genetic engineering ; Genetic vectors ; Genetic Vectors - genetics ; Health aspects ; Health. Pharmaceutical industry ; Humans ; Immunization ; Immunology ; Industrial applications and implications. Economical aspects ; Injection ; Lectins, C-Type - metabolism ; Lentivirus - genetics ; Life Sciences ; Methods ; Mice ; Mice, Inbred C57BL ; Mice, Transgenic ; Miscellaneous ; Neoplasms, Experimental - immunology ; Neoplasms, Experimental - therapy ; Ovalbumin - genetics ; Ovalbumin - immunology ; Proteins ; Receptors, Cell Surface - metabolism ; Research methodology ; Sensitivity and Specificity ; Sindbis virus ; Sindbis Virus - genetics ; Sindbis Virus - metabolism ; Transduction, Genetic ; Tumors</subject><ispartof>Nature biotechnology, 2008-03, Vol.26 (3), p.326-334</ispartof><rights>Springer Nature America, Inc. 2008</rights><rights>2008 INIST-CNRS</rights><rights>COPYRIGHT 2008 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group Mar 2008</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c661t-5237ca82f074cd8ab9ea1a500b3a88bf2abd93f2bdab75dc8df857ed49a63c163</citedby><cites>FETCH-LOGICAL-c661t-5237ca82f074cd8ab9ea1a500b3a88bf2abd93f2bdab75dc8df857ed49a63c163</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/nbt1390$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/nbt1390$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>230,314,776,780,881,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20414237$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18297056$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yang, Lili</creatorcontrib><creatorcontrib>Yang, Haiguang</creatorcontrib><creatorcontrib>Rideout, Kendra</creatorcontrib><creatorcontrib>Cho, Taehoon</creatorcontrib><creatorcontrib>Joo, Kye il</creatorcontrib><creatorcontrib>Ziegler, Leslie</creatorcontrib><creatorcontrib>Elliot, Abigail</creatorcontrib><creatorcontrib>Walls, Anthony</creatorcontrib><creatorcontrib>Yu, Dongzi</creatorcontrib><creatorcontrib>Baltimore, David</creatorcontrib><creatorcontrib>Wang, Pin</creatorcontrib><title>Engineered lentivector targeting of dendritic cells for in vivo immunization</title><title>Nature biotechnology</title><addtitle>Nat Biotechnol</addtitle><addtitle>Nat Biotechnol</addtitle><description>We report a method of inducing antigen production in dendritic cells by
in vivo
targeting with lentiviral vectors that specifically bind to the dendritic cell–surface protein DC-SIGN. To target dendritic cells, we enveloped the lentivector with a viral glycoprotein from Sindbis virus engineered to be DC-SIGN–specific.
In vitro
, this lentivector specifically transduced dendritic cells and induced dendritic cell maturation. A high frequency (up to 12%) of ovalbumin (OVA)-specific CD8
+
T cells and a significant antibody response were observed 2 weeks after injection of a targeted lentiviral vector encoding an OVA transgene into naive mice. This approach also protected against the growth of OVA-expressing E.G7 tumors and induced regression of established tumors. Thus, lentiviral vectors targeting dendritic cells provide a simple method of producing effective immunity and may provide an alternative route for immunization with protein antigens.</description><subject>Agriculture</subject><subject>Animals</subject><subject>B-Lymphocytes - cytology</subject><subject>B-Lymphocytes - immunology</subject><subject>Bioinformatics</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedical Engineering/Biotechnology</subject><subject>Biomedicine</subject><subject>Biotechnology</subject><subject>Cancer Vaccines - immunology</subject><subject>CD8-Positive T-Lymphocytes - cytology</subject><subject>CD8-Positive T-Lymphocytes - immunology</subject><subject>Cell Adhesion Molecules - metabolism</subject><subject>Cell Line</subject><subject>Cellular biology</subject><subject>Dendritic cells</subject><subject>Dendritic Cells - cytology</subject><subject>Dendritic Cells - immunology</subject><subject>Dendritic Cells - metabolism</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>Genetic aspects</subject><subject>Genetic engineering</subject><subject>Genetic vectors</subject><subject>Genetic Vectors - genetics</subject><subject>Health aspects</subject><subject>Health. Pharmaceutical industry</subject><subject>Humans</subject><subject>Immunization</subject><subject>Immunology</subject><subject>Industrial applications and implications. Economical aspects</subject><subject>Injection</subject><subject>Lectins, C-Type - metabolism</subject><subject>Lentivirus - genetics</subject><subject>Life Sciences</subject><subject>Methods</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Transgenic</subject><subject>Miscellaneous</subject><subject>Neoplasms, Experimental - immunology</subject><subject>Neoplasms, Experimental - therapy</subject><subject>Ovalbumin - genetics</subject><subject>Ovalbumin - immunology</subject><subject>Proteins</subject><subject>Receptors, Cell Surface - metabolism</subject><subject>Research methodology</subject><subject>Sensitivity and Specificity</subject><subject>Sindbis virus</subject><subject>Sindbis Virus - genetics</subject><subject>Sindbis Virus - metabolism</subject><subject>Transduction, Genetic</subject><subject>Tumors</subject><issn>1087-0156</issn><issn>1546-1696</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqN0klv1DAUB_AIgegC4hugCIntMMVL4tgXpKoqUGmkSmxX68VLcJXYre2MKJ8el4laphdIDonin19e_nlV9QyjI4wof-f7jKlAD6p93DZshZlgD8s94t0K4ZbtVQcpXSCEWMPY42oPcyI61LL9an3qB-eNiUbXo_HZbYzKIdYZ4mCy80MdbK2N19Flp2plxjHVtgDn643bhNpN0-zdL8gu-CfVIwtjMk-X62H17cPp15NPq_X5x7OT4_VKMYbzqiW0U8CJRV2jNIdeGMDQItRT4Ly3BHotqCW9hr5rteLa8rYzuhHAqMKMHlbvt3Uv534yWpW-I4zyMroJ4rUM4OTuinc_5BA2ktDSACOlwKulQAxXs0lZTi7dfBt4E-Yku5Ip5Yj-ExLEBWk5LvDFPXgR5uhLCpKUg1PRiYKOtmiA0UjnbSjdqXJqMzkVvLGuPD_GgjddQ_5UfbuzoZhsfuYB5pTk2ZfP_2_Pv-_a11urYkgpGnubHUbyZqDkMlBFPv876ju3TFABLxcAScFoI3jl0q0jqMFN-d_Fvdm6VJb8YOJdQPff-RvfRuAh</recordid><startdate>20080301</startdate><enddate>20080301</enddate><creator>Yang, Lili</creator><creator>Yang, Haiguang</creator><creator>Rideout, Kendra</creator><creator>Cho, Taehoon</creator><creator>Joo, Kye il</creator><creator>Ziegler, Leslie</creator><creator>Elliot, Abigail</creator><creator>Walls, Anthony</creator><creator>Yu, Dongzi</creator><creator>Baltimore, David</creator><creator>Wang, Pin</creator><general>Nature Publishing Group US</general><general>Nature</general><general>Nature Publishing Group</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QO</scope><scope>7QP</scope><scope>7QR</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M2P</scope><scope>M7P</scope><scope>M7S</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>Q9U</scope><scope>RC3</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20080301</creationdate><title>Engineered lentivector targeting of dendritic cells for in vivo immunization</title><author>Yang, Lili ; Yang, Haiguang ; Rideout, Kendra ; Cho, Taehoon ; Joo, Kye il ; Ziegler, Leslie ; Elliot, Abigail ; Walls, Anthony ; Yu, Dongzi ; Baltimore, David ; Wang, Pin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c661t-5237ca82f074cd8ab9ea1a500b3a88bf2abd93f2bdab75dc8df857ed49a63c163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Agriculture</topic><topic>Animals</topic><topic>B-Lymphocytes - cytology</topic><topic>B-Lymphocytes - immunology</topic><topic>Bioinformatics</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedical Engineering/Biotechnology</topic><topic>Biomedicine</topic><topic>Biotechnology</topic><topic>Cancer Vaccines - immunology</topic><topic>CD8-Positive T-Lymphocytes - cytology</topic><topic>CD8-Positive T-Lymphocytes - immunology</topic><topic>Cell Adhesion Molecules - metabolism</topic><topic>Cell Line</topic><topic>Cellular biology</topic><topic>Dendritic cells</topic><topic>Dendritic Cells - cytology</topic><topic>Dendritic Cells - immunology</topic><topic>Dendritic Cells - metabolism</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>Genetic aspects</topic><topic>Genetic engineering</topic><topic>Genetic vectors</topic><topic>Genetic Vectors - genetics</topic><topic>Health aspects</topic><topic>Health. Pharmaceutical industry</topic><topic>Humans</topic><topic>Immunization</topic><topic>Immunology</topic><topic>Industrial applications and implications. Economical aspects</topic><topic>Injection</topic><topic>Lectins, C-Type - metabolism</topic><topic>Lentivirus - genetics</topic><topic>Life Sciences</topic><topic>Methods</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Transgenic</topic><topic>Miscellaneous</topic><topic>Neoplasms, Experimental - immunology</topic><topic>Neoplasms, Experimental - therapy</topic><topic>Ovalbumin - genetics</topic><topic>Ovalbumin - immunology</topic><topic>Proteins</topic><topic>Receptors, Cell Surface - metabolism</topic><topic>Research methodology</topic><topic>Sensitivity and Specificity</topic><topic>Sindbis virus</topic><topic>Sindbis Virus - genetics</topic><topic>Sindbis Virus - metabolism</topic><topic>Transduction, Genetic</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yang, Lili</creatorcontrib><creatorcontrib>Yang, Haiguang</creatorcontrib><creatorcontrib>Rideout, Kendra</creatorcontrib><creatorcontrib>Cho, Taehoon</creatorcontrib><creatorcontrib>Joo, Kye il</creatorcontrib><creatorcontrib>Ziegler, Leslie</creatorcontrib><creatorcontrib>Elliot, Abigail</creatorcontrib><creatorcontrib>Walls, Anthony</creatorcontrib><creatorcontrib>Yu, Dongzi</creatorcontrib><creatorcontrib>Baltimore, David</creatorcontrib><creatorcontrib>Wang, Pin</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nature biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yang, Lili</au><au>Yang, Haiguang</au><au>Rideout, Kendra</au><au>Cho, Taehoon</au><au>Joo, Kye il</au><au>Ziegler, Leslie</au><au>Elliot, Abigail</au><au>Walls, Anthony</au><au>Yu, Dongzi</au><au>Baltimore, David</au><au>Wang, Pin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Engineered lentivector targeting of dendritic cells for in vivo immunization</atitle><jtitle>Nature biotechnology</jtitle><stitle>Nat Biotechnol</stitle><addtitle>Nat Biotechnol</addtitle><date>2008-03-01</date><risdate>2008</risdate><volume>26</volume><issue>3</issue><spage>326</spage><epage>334</epage><pages>326-334</pages><issn>1087-0156</issn><eissn>1546-1696</eissn><coden>NABIF9</coden><abstract>We report a method of inducing antigen production in dendritic cells by
in vivo
targeting with lentiviral vectors that specifically bind to the dendritic cell–surface protein DC-SIGN. To target dendritic cells, we enveloped the lentivector with a viral glycoprotein from Sindbis virus engineered to be DC-SIGN–specific.
In vitro
, this lentivector specifically transduced dendritic cells and induced dendritic cell maturation. A high frequency (up to 12%) of ovalbumin (OVA)-specific CD8
+
T cells and a significant antibody response were observed 2 weeks after injection of a targeted lentiviral vector encoding an OVA transgene into naive mice. This approach also protected against the growth of OVA-expressing E.G7 tumors and induced regression of established tumors. Thus, lentiviral vectors targeting dendritic cells provide a simple method of producing effective immunity and may provide an alternative route for immunization with protein antigens.</abstract><cop>New York</cop><pub>Nature Publishing Group US</pub><pmid>18297056</pmid><doi>10.1038/nbt1390</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1087-0156 |
| ispartof | Nature biotechnology, 2008-03, Vol.26 (3), p.326-334 |
| issn | 1087-0156 1546-1696 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2366162 |
| source | MEDLINE; Nature; SpringerNature Complete Journals |
| subjects | Agriculture Animals B-Lymphocytes - cytology B-Lymphocytes - immunology Bioinformatics Biological and medical sciences Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biomedicine Biotechnology Cancer Vaccines - immunology CD8-Positive T-Lymphocytes - cytology CD8-Positive T-Lymphocytes - immunology Cell Adhesion Molecules - metabolism Cell Line Cellular biology Dendritic cells Dendritic Cells - cytology Dendritic Cells - immunology Dendritic Cells - metabolism Female Fundamental and applied biological sciences. Psychology Gene Expression Genetic aspects Genetic engineering Genetic vectors Genetic Vectors - genetics Health aspects Health. Pharmaceutical industry Humans Immunization Immunology Industrial applications and implications. Economical aspects Injection Lectins, C-Type - metabolism Lentivirus - genetics Life Sciences Methods Mice Mice, Inbred C57BL Mice, Transgenic Miscellaneous Neoplasms, Experimental - immunology Neoplasms, Experimental - therapy Ovalbumin - genetics Ovalbumin - immunology Proteins Receptors, Cell Surface - metabolism Research methodology Sensitivity and Specificity Sindbis virus Sindbis Virus - genetics Sindbis Virus - metabolism Transduction, Genetic Tumors |
| title | Engineered lentivector targeting of dendritic cells for in vivo immunization |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-04T07%3A56%3A35IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Engineered%20lentivector%20targeting%20of%20dendritic%20cells%20for%20in%20vivo%20immunization&rft.jtitle=Nature%20biotechnology&rft.au=Yang,%20Lili&rft.date=2008-03-01&rft.volume=26&rft.issue=3&rft.spage=326&rft.epage=334&rft.pages=326-334&rft.issn=1087-0156&rft.eissn=1546-1696&rft.coden=NABIF9&rft_id=info:doi/10.1038/nbt1390&rft_dat=%3Cgale_pubme%3EA198474281%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=222283979&rft_id=info:pmid/18297056&rft_galeid=A198474281&rfr_iscdi=true |