Association of p53 codon 72 polymorphism with advanced lung cancer: the Arg allele is preferentially retained in tumours arising in Arg/Pro germline heterozygotes
The association of p53 codon 72 polymorphism with cancer has been investigated by several scientific groups with controversial results. In the present study, we examined the genotypic frequency of this polymorphism in 54 patients with advanced lung cancer and 99 normal controls from the geographical...
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| Veröffentlicht in: | British journal of cancer 2002-10, Vol.87 (9), p.1013-1018 |
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| creator | Papadakis, E D Soulitzis, N Spandidos, D A |
| description | The association of
p53
codon 72 polymorphism with cancer has been investigated by several scientific groups with controversial results. In the present study, we examined the genotypic frequency of this polymorphism in 54 patients with advanced lung cancer and 99 normal controls from the geographical region of Greece. Sputum and bronchial washing samples from each patient were assayed for the presence of human papillomavirus. Codon 72 heterozygous (Arg/Pro) patients were also analysed for loss of heterozygosity at the
TP53
locus, in order to determine the lost
p53
allele (Arg or Pro).
p53
Arg/Arg genotype was significantly increased in lung cancer patients compared to normal controls (50%
vs
24.2%,
P |
| doi_str_mv | 10.1038/sj.bjc.6600595 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2364333</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>18619012</sourcerecordid><originalsourceid>FETCH-LOGICAL-c546t-c858e1b70907740fcd921f7ea69b7c73839aa40be84737083c7993687984096b3</originalsourceid><addsrcrecordid>eNp9kkurEzEUx4Mo3lrdupQgqKu2ec0kuQuhXHzBBV3oOmTSTJuSmYzJzJX6cfykntJiVdBskpP8zjN_hJ5SsqSEq1XZL5u9W9Y1IZWu7qEZrThbUMXkfTQjhMgF0YxcoUel7MHURMmH6IoywQXTYoZ-rEtJLtgxpB6nFg8Vxy5twJAMDykeupSHXSgd_hbGHbabO9s7v8Fx6rfYHc_5Go87j9d5i22MPnocCh6yb332_Rjg7oCzH23owS30eJy6NOWCbQ4lQBC4At_Vp5zw1ucuAod3fvQ5fT9s0-jLY_SgtbH4J-d9jr68ffP55v3i9uO7Dzfr24WrRD0unKqUp42EHqUUpHUbzWgrva11I53kimtrBWm8EpJLoriTWvNaSa0E0XXD5-j1Ke4wNZ3fOKg-22iGHDqbDybZYP586cPObNOdYbwWHNYcvToHyOnr5MtoulCcj9H2Pk3FKCIZpNYCyJf_JamqqSaUAfj8L3APw-thDIYxQjmh0MgcLU-Qy6kUmPyvmikxR5WYsjegEnNWCTg8-73TC36WBQAvzoAtzsY2w0eHcuEEKImLY-bViSvw1MP3Xcr7R-qfZFDXzA</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>220130108</pqid></control><display><type>article</type><title>Association of p53 codon 72 polymorphism with advanced lung cancer: the Arg allele is preferentially retained in tumours arising in Arg/Pro germline heterozygotes</title><source>MEDLINE</source><source>Nature</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>Papadakis, E D ; Soulitzis, N ; Spandidos, D A</creator><creatorcontrib>Papadakis, E D ; Soulitzis, N ; Spandidos, D A</creatorcontrib><description>The association of
p53
codon 72 polymorphism with cancer has been investigated by several scientific groups with controversial results. In the present study, we examined the genotypic frequency of this polymorphism in 54 patients with advanced lung cancer and 99 normal controls from the geographical region of Greece. Sputum and bronchial washing samples from each patient were assayed for the presence of human papillomavirus. Codon 72 heterozygous (Arg/Pro) patients were also analysed for loss of heterozygosity at the
TP53
locus, in order to determine the lost
p53
allele (Arg or Pro).
p53
Arg/Arg genotype was significantly increased in lung cancer patients compared to normal controls (50%
vs
24.2%,
P
<0.002). Human papillomavirus was detected only in two patients (3.7%). Loss of heterozygosity at the
TP53
locus was found in 14 out of 27 Arg/Pro patients (51.85%). The Pro allele was lost in 11 cases (78.6%), while the Arg allele was lost in three (21.4%). Our results suggest that
p53
codon 72 Arg homozygosity is associated with advanced lung cancer, and that the Arg allele is preferentially retained in patients heterozygous for this polymorphism. On the other hand, human papillomavirus infection does not seem to play an important role in lung carcinogenesis.</description><identifier>ISSN: 0007-0920</identifier><identifier>EISSN: 1532-1827</identifier><identifier>DOI: 10.1038/sj.bjc.6600595</identifier><identifier>PMID: 12434294</identifier><identifier>CODEN: BJCAAI</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Aged ; Arginine - genetics ; Biological and medical sciences ; Biomedical and Life Sciences ; Biomedicine ; Bronchoalveolar Lavage Fluid - virology ; Cancer Research ; Codon ; DNA Primers - chemistry ; Drug Resistance ; Epidemiology ; Female ; Gene Deletion ; Genes, p53 ; Genotype ; Germ-Line Mutation ; Heterozygote ; Human papillomavirus ; Humans ; Loss of Heterozygosity ; Lung Neoplasms - genetics ; Lung Neoplasms - virology ; Male ; Medical sciences ; Middle Aged ; Molecular and Cellular Pathology ; Molecular Medicine ; Oncology ; Papillomaviridae - isolation & purification ; Papillomavirus Infections - diagnosis ; Pneumology ; Polymorphism, Genetic ; Proline - genetics ; Sputum - virology ; Tumor Suppressor Protein p53 - genetics ; Tumor Virus Infections - diagnosis ; Tumors of the respiratory system and mediastinum</subject><ispartof>British journal of cancer, 2002-10, Vol.87 (9), p.1013-1018</ispartof><rights>The Author(s) 2002</rights><rights>2003 INIST-CNRS</rights><rights>Copyright Nature Publishing Group Oct 21, 2002</rights><rights>Copyright © 2002 Cancer Research UK 2002 Cancer Research UK</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c546t-c858e1b70907740fcd921f7ea69b7c73839aa40be84737083c7993687984096b3</citedby><cites>FETCH-LOGICAL-c546t-c858e1b70907740fcd921f7ea69b7c73839aa40be84737083c7993687984096b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2364333/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2364333/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14009348$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12434294$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Papadakis, E D</creatorcontrib><creatorcontrib>Soulitzis, N</creatorcontrib><creatorcontrib>Spandidos, D A</creatorcontrib><title>Association of p53 codon 72 polymorphism with advanced lung cancer: the Arg allele is preferentially retained in tumours arising in Arg/Pro germline heterozygotes</title><title>British journal of cancer</title><addtitle>Br J Cancer</addtitle><addtitle>Br J Cancer</addtitle><description>The association of
p53
codon 72 polymorphism with cancer has been investigated by several scientific groups with controversial results. In the present study, we examined the genotypic frequency of this polymorphism in 54 patients with advanced lung cancer and 99 normal controls from the geographical region of Greece. Sputum and bronchial washing samples from each patient were assayed for the presence of human papillomavirus. Codon 72 heterozygous (Arg/Pro) patients were also analysed for loss of heterozygosity at the
TP53
locus, in order to determine the lost
p53
allele (Arg or Pro).
p53
Arg/Arg genotype was significantly increased in lung cancer patients compared to normal controls (50%
vs
24.2%,
P
<0.002). Human papillomavirus was detected only in two patients (3.7%). Loss of heterozygosity at the
TP53
locus was found in 14 out of 27 Arg/Pro patients (51.85%). The Pro allele was lost in 11 cases (78.6%), while the Arg allele was lost in three (21.4%). Our results suggest that
p53
codon 72 Arg homozygosity is associated with advanced lung cancer, and that the Arg allele is preferentially retained in patients heterozygous for this polymorphism. On the other hand, human papillomavirus infection does not seem to play an important role in lung carcinogenesis.</description><subject>Aged</subject><subject>Arginine - genetics</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Bronchoalveolar Lavage Fluid - virology</subject><subject>Cancer Research</subject><subject>Codon</subject><subject>DNA Primers - chemistry</subject><subject>Drug Resistance</subject><subject>Epidemiology</subject><subject>Female</subject><subject>Gene Deletion</subject><subject>Genes, p53</subject><subject>Genotype</subject><subject>Germ-Line Mutation</subject><subject>Heterozygote</subject><subject>Human papillomavirus</subject><subject>Humans</subject><subject>Loss of Heterozygosity</subject><subject>Lung Neoplasms - genetics</subject><subject>Lung Neoplasms - virology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Molecular and Cellular Pathology</subject><subject>Molecular Medicine</subject><subject>Oncology</subject><subject>Papillomaviridae - isolation & purification</subject><subject>Papillomavirus Infections - diagnosis</subject><subject>Pneumology</subject><subject>Polymorphism, Genetic</subject><subject>Proline - genetics</subject><subject>Sputum - virology</subject><subject>Tumor Suppressor Protein p53 - genetics</subject><subject>Tumor Virus Infections - diagnosis</subject><subject>Tumors of the respiratory system and mediastinum</subject><issn>0007-0920</issn><issn>1532-1827</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kkurEzEUx4Mo3lrdupQgqKu2ec0kuQuhXHzBBV3oOmTSTJuSmYzJzJX6cfykntJiVdBskpP8zjN_hJ5SsqSEq1XZL5u9W9Y1IZWu7qEZrThbUMXkfTQjhMgF0YxcoUel7MHURMmH6IoywQXTYoZ-rEtJLtgxpB6nFg8Vxy5twJAMDykeupSHXSgd_hbGHbabO9s7v8Fx6rfYHc_5Go87j9d5i22MPnocCh6yb332_Rjg7oCzH23owS30eJy6NOWCbQ4lQBC4At_Vp5zw1ucuAod3fvQ5fT9s0-jLY_SgtbH4J-d9jr68ffP55v3i9uO7Dzfr24WrRD0unKqUp42EHqUUpHUbzWgrva11I53kimtrBWm8EpJLoriTWvNaSa0E0XXD5-j1Ke4wNZ3fOKg-22iGHDqbDybZYP586cPObNOdYbwWHNYcvToHyOnr5MtoulCcj9H2Pk3FKCIZpNYCyJf_JamqqSaUAfj8L3APw-thDIYxQjmh0MgcLU-Qy6kUmPyvmikxR5WYsjegEnNWCTg8-73TC36WBQAvzoAtzsY2w0eHcuEEKImLY-bViSvw1MP3Xcr7R-qfZFDXzA</recordid><startdate>20021021</startdate><enddate>20021021</enddate><creator>Papadakis, E D</creator><creator>Soulitzis, N</creator><creator>Spandidos, D A</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AN0</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>5PM</scope></search><sort><creationdate>20021021</creationdate><title>Association of p53 codon 72 polymorphism with advanced lung cancer: the Arg allele is preferentially retained in tumours arising in Arg/Pro germline heterozygotes</title><author>Papadakis, E D ; Soulitzis, N ; Spandidos, D A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c546t-c858e1b70907740fcd921f7ea69b7c73839aa40be84737083c7993687984096b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Aged</topic><topic>Arginine - genetics</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Bronchoalveolar Lavage Fluid - virology</topic><topic>Cancer Research</topic><topic>Codon</topic><topic>DNA Primers - chemistry</topic><topic>Drug Resistance</topic><topic>Epidemiology</topic><topic>Female</topic><topic>Gene Deletion</topic><topic>Genes, p53</topic><topic>Genotype</topic><topic>Germ-Line Mutation</topic><topic>Heterozygote</topic><topic>Human papillomavirus</topic><topic>Humans</topic><topic>Loss of Heterozygosity</topic><topic>Lung Neoplasms - genetics</topic><topic>Lung Neoplasms - virology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Molecular and Cellular Pathology</topic><topic>Molecular Medicine</topic><topic>Oncology</topic><topic>Papillomaviridae - isolation & purification</topic><topic>Papillomavirus Infections - diagnosis</topic><topic>Pneumology</topic><topic>Polymorphism, Genetic</topic><topic>Proline - genetics</topic><topic>Sputum - virology</topic><topic>Tumor Suppressor Protein p53 - genetics</topic><topic>Tumor Virus Infections - diagnosis</topic><topic>Tumors of the respiratory system and mediastinum</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Papadakis, E D</creatorcontrib><creatorcontrib>Soulitzis, N</creatorcontrib><creatorcontrib>Spandidos, D A</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>British Nursing Database</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>British journal of cancer</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Papadakis, E D</au><au>Soulitzis, N</au><au>Spandidos, D A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Association of p53 codon 72 polymorphism with advanced lung cancer: the Arg allele is preferentially retained in tumours arising in Arg/Pro germline heterozygotes</atitle><jtitle>British journal of cancer</jtitle><stitle>Br J Cancer</stitle><addtitle>Br J Cancer</addtitle><date>2002-10-21</date><risdate>2002</risdate><volume>87</volume><issue>9</issue><spage>1013</spage><epage>1018</epage><pages>1013-1018</pages><issn>0007-0920</issn><eissn>1532-1827</eissn><coden>BJCAAI</coden><abstract>The association of
p53
codon 72 polymorphism with cancer has been investigated by several scientific groups with controversial results. In the present study, we examined the genotypic frequency of this polymorphism in 54 patients with advanced lung cancer and 99 normal controls from the geographical region of Greece. Sputum and bronchial washing samples from each patient were assayed for the presence of human papillomavirus. Codon 72 heterozygous (Arg/Pro) patients were also analysed for loss of heterozygosity at the
TP53
locus, in order to determine the lost
p53
allele (Arg or Pro).
p53
Arg/Arg genotype was significantly increased in lung cancer patients compared to normal controls (50%
vs
24.2%,
P
<0.002). Human papillomavirus was detected only in two patients (3.7%). Loss of heterozygosity at the
TP53
locus was found in 14 out of 27 Arg/Pro patients (51.85%). The Pro allele was lost in 11 cases (78.6%), while the Arg allele was lost in three (21.4%). Our results suggest that
p53
codon 72 Arg homozygosity is associated with advanced lung cancer, and that the Arg allele is preferentially retained in patients heterozygous for this polymorphism. On the other hand, human papillomavirus infection does not seem to play an important role in lung carcinogenesis.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>12434294</pmid><doi>10.1038/sj.bjc.6600595</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Aged Arginine - genetics Biological and medical sciences Biomedical and Life Sciences Biomedicine Bronchoalveolar Lavage Fluid - virology Cancer Research Codon DNA Primers - chemistry Drug Resistance Epidemiology Female Gene Deletion Genes, p53 Genotype Germ-Line Mutation Heterozygote Human papillomavirus Humans Loss of Heterozygosity Lung Neoplasms - genetics Lung Neoplasms - virology Male Medical sciences Middle Aged Molecular and Cellular Pathology Molecular Medicine Oncology Papillomaviridae - isolation & purification Papillomavirus Infections - diagnosis Pneumology Polymorphism, Genetic Proline - genetics Sputum - virology Tumor Suppressor Protein p53 - genetics Tumor Virus Infections - diagnosis Tumors of the respiratory system and mediastinum |
| title | Association of p53 codon 72 polymorphism with advanced lung cancer: the Arg allele is preferentially retained in tumours arising in Arg/Pro germline heterozygotes |
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