Quantitative PCR technique for detecting lymphocytic choriomeningitis virus in vivo

Quantitative PCR (QPCR, or real time PCR (rtPCR)) has emerged as a powerful virologic technique for measuring viral replication and viral loads in humans and animal models. We have developed a QPCR assay to accurately quantify lymphocytic choriomeningitis virus (LCMV) in infected mice. We first vali...

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Veröffentlicht in:	Journal of virological methods 2008-01, Vol.147 (1), p.167-176
Hauptverfasser:	McCausland, Megan M., Crotty, Shane
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Arenavirus Biological and medical sciences Chronic infection Fundamental and applied biological sciences. Psychology Lymphocytic Choriomeningitis - diagnosis Lymphocytic Choriomeningitis - virology Lymphocytic choriomeningitis virus Lymphocytic choriomeningitis virus - isolation & purification Mice Mice, Inbred C57BL Mice, Mutant Strains Microbiology Polymerase Chain Reaction - methods RNA virus RT-PCR rtPCR Sensitivity and Specificity Techniques used in virology Virology
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