Amphiphilic biopolymers (amphibiopols) as new surfactants for membrane protein solubilization

The aim of this study was to develop new surfactants for membrane protein solubilization, from a natural, biodegradable polymer: the polysaccharide pullulan. A set of amphiphilic pullulans (HMCMPs), differing in hydrophobic modification ratio, charge ratio, and the nature of the hydrophobic chains i...

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Veröffentlicht in:	Protein science 2003-04, Vol.12 (4), p.681-689
Hauptverfasser:	Duval‐Terrié, Caroline, Cosette, Pascal, Molle, Gérard, Muller, Guy, Dé, Emmanuelle
Format:	Artikel
Sprache:	eng
Schlagworte:	amphiphilic polysaccharide Bacteria - chemistry Bacteria - metabolism BR, bacteriorhodopsin Chemical Sciences Circular Dichroism HMCMP, hydrophobically modified carboxymethylpullulan Hydrophobic and Hydrophilic Interactions LiDS, lithium dodecylsulfate Material chemistry Membrane protein Membrane Proteins - chemistry Octyl‐POE, octylpolyoxyethylene polymeric surfactant Polymers Porins - metabolism pullulan Solubility solubilization Surface-Active Agents - chemistry Tris‐HCl, Tris(hydroxymethyl)aminomethane hydrochloride
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