Proof for a Nonproteinaceous Calcium-Selective Channel in Escherichia coli by Total Synthesis from (R)-3-hydroxybutanoic Acid and Inorganic Polyphosphate
Traditionally, the structure and properties of natural products have been determined by total synthesis and comparison with authentic samples. We have now applied this procedure to the first nonproteinaceous ion channel, isolated from bacterial plasma membranes, and consisting of a complex of poly(3...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1997-08, Vol.94 (17), p.9075-9079 |
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| container_issue | 17 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 94 |
| creator | Das, Sudipto Lengweiler, Urs D. Seebach, Dieter Reusch, Rosetta N. |
| description | Traditionally, the structure and properties of natural products have been determined by total synthesis and comparison with authentic samples. We have now applied this procedure to the first nonproteinaceous ion channel, isolated from bacterial plasma membranes, and consisting of a complex of poly(3-hydroxybutyrate) and calcium polyphosphate. To this end, we have now synthesized the 128-mer of hydroxybutanoic acid and prepared a complex with inorganic calcium polyphosphate (average 65-mer), which was incorporated into a planar lipid bilayer of synthetic phospholipids. We herewith present data that demonstrate unambiguously that the completely synthetic complex forms channels that are indistinguishable in their voltage-dependent conductance, in their selectivity for divalent cations, and in their blocking behavior (by La3+) from channels isolated from Escherichia coli. The implications of our finding for prebiotic chemistry, biochemistry, and biology are discussed. |
| doi_str_mv | 10.1073/pnas.94.17.9075 |
| format | Article |
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The implications of our finding for prebiotic chemistry, biochemistry, and biology are discussed.</description><subject>Bathing</subject><subject>Biological Sciences</subject><subject>Biophysics</subject><subject>Calcium</subject><subject>Calcium Channels - chemical synthesis</subject><subject>Calcium Channels - chemistry</subject><subject>Chlorides</subject><subject>Escherichia coli</subject><subject>Escherichia coli - chemistry</subject><subject>Escherichia coli - metabolism</subject><subject>Hydroxybutyrates</subject><subject>Ion channels</subject><subject>Lipid bilayers</subject><subject>Polymers</subject><subject>Polyphosphates</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU9rFDEYxoModV09C4KSk38Os00m8y_gpSxVC0WLreeQybzppGSSaZIpnY_it3WWXRa9mEsgz-95E_JD6DUlG0pqdjo6GTe82NB6w0ldPkErSjjNqoKTp2hFSF5nTZEXz9GLGO8IIbxsyAk64XlZFaxeod9XwXuNtQ9Y4u_ejcEnME4q8FPEW2mVmYbsGiyoZB4Ab3vpHFhsHD6PqodgVG8kVt4a3M74xidp8fXsUg_RRKyDH_DHn58ylvVzF_zj3E5JOm8UPlOmw9J1-ML5cCvdcnTl7Tz2Po69TPASPdPSRnh12Nfo15fzm-237PLH14vt2WWmioqkjBMCbZ3zFkDXpGNtRbuuIZotS-u8aVTLKasIUFU3LbCu0zmThFKpadM0kq3R5_3ccWoH6BS4FKQVYzCDDLPw0oh_E2d6cesfRM4Iq5b6-0M9-PsJYhKDiQqslW73haLmOaXNomqNTvegCj7GAPp4BSVi51LsXApeCFqLncul8fbvlx35g7wl_3DId8Vjehwg9GRtgse0kO_-Sy7Amz1wF5MPR6LIy5KzP_2rwDE</recordid><startdate>19970819</startdate><enddate>19970819</enddate><creator>Das, Sudipto</creator><creator>Lengweiler, Urs D.</creator><creator>Seebach, Dieter</creator><creator>Reusch, Rosetta N.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><general>The National Academy of Sciences of the USA</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19970819</creationdate><title>Proof for a Nonproteinaceous Calcium-Selective Channel in Escherichia coli by Total Synthesis from (R)-3-hydroxybutanoic Acid and Inorganic Polyphosphate</title><author>Das, Sudipto ; Lengweiler, Urs D. ; Seebach, Dieter ; Reusch, Rosetta N.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c460t-900eb729beef70d3b61dd80f3333ff288cb91360e1c78be3ddf23a011af1888a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Bathing</topic><topic>Biological Sciences</topic><topic>Biophysics</topic><topic>Calcium</topic><topic>Calcium Channels - chemical synthesis</topic><topic>Calcium Channels - chemistry</topic><topic>Chlorides</topic><topic>Escherichia coli</topic><topic>Escherichia coli - chemistry</topic><topic>Escherichia coli - metabolism</topic><topic>Hydroxybutyrates</topic><topic>Ion channels</topic><topic>Lipid bilayers</topic><topic>Polymers</topic><topic>Polyphosphates</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Das, Sudipto</creatorcontrib><creatorcontrib>Lengweiler, Urs D.</creatorcontrib><creatorcontrib>Seebach, Dieter</creatorcontrib><creatorcontrib>Reusch, Rosetta N.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Das, Sudipto</au><au>Lengweiler, Urs D.</au><au>Seebach, Dieter</au><au>Reusch, Rosetta N.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Proof for a Nonproteinaceous Calcium-Selective Channel in Escherichia coli by Total Synthesis from (R)-3-hydroxybutanoic Acid and Inorganic Polyphosphate</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1997-08-19</date><risdate>1997</risdate><volume>94</volume><issue>17</issue><spage>9075</spage><epage>9079</epage><pages>9075-9079</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>Traditionally, the structure and properties of natural products have been determined by total synthesis and comparison with authentic samples. 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| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1997-08, Vol.94 (17), p.9075-9079 |
| issn | 0027-8424 1091-6490 |
| language | eng |
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| source | MEDLINE; JSTOR Complete Journals; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Bathing Biological Sciences Biophysics Calcium Calcium Channels - chemical synthesis Calcium Channels - chemistry Chlorides Escherichia coli Escherichia coli - chemistry Escherichia coli - metabolism Hydroxybutyrates Ion channels Lipid bilayers Polymers Polyphosphates |
| title | Proof for a Nonproteinaceous Calcium-Selective Channel in Escherichia coli by Total Synthesis from (R)-3-hydroxybutanoic Acid and Inorganic Polyphosphate |
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