Subtyping Listeria monocytogenes isolates genetically related to the Swiss epidemic clone
Macrorestriction analysis by pulsed-field gel electrophoresis was used to assess the diversity of strains within the epidemic-associated electrophoretic type 1 (ET1) clone of Listeria monocytogenes. For this purpose, a total of 144 isolates from Switzerland shown by multilocus enzyme electrophoresis...
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| Veröffentlicht in: | Journal of Clinical Microbiology 1996-09, Vol.34 (9), p.2148-2153 |
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| creator | Boerlin, P. (University of Guelph, Guelph, Canada.) Bannerman, E Jemmi, T Bille, J |
| description | Macrorestriction analysis by pulsed-field gel electrophoresis was used to assess the diversity of strains within the epidemic-associated electrophoretic type 1 (ET1) clone of Listeria monocytogenes. For this purpose, a total of 144 isolates from Switzerland shown by multilocus enzyme electrophoresis to belong to the ET1 were examined. These isolates were subtyped by macrorestriction analysis using the enzymes ApaI and SmaI and field inversion gel electrophoresis. Among these 144 isolates, 45 were isolated in human listeriosis cases of the postepidemic period of 1988 to 1993 and 44 were isolated in animal listeriosis cases of the same period. Forty-seven isolates were from the epidemic period of 1983 to 1987, and eight additional isolates were from cattle from two different farms. Twenty-nine different subtypes could be identified among the 144 isolates tested. Five major subtypes were found more frequently than the others during the postepidemic period, both in humans and in animals. Two of these subtypes had been previously implicated in outbreaks of listeriosis, thus suggesting that particular pulsed-field gel electrophoresis subtypes may be frequently associated with disease in humans and animals. Two of these frequent subtypes were also suspected to be related to small clusters of listeriosis cases during the postepidemic period. The results obtained by typing epidemiologically related isolates from different animals within the same farms and from different body sites of a given patient confirmed the potential of macrorestriction analysis for epidemiological studies restricted to short periods of time and to small number of isolates. The analysis of 47 isolates related to the Swiss listeriosis epidemic period of 1983 to 1987 and the use of Southern blotting and hybridisation experiments show that the interpretation of relatedness between isolates presenting slightly different macrorestriction patterns may be more complete than commonly accepted. In such cases, c |
| doi_str_mv | 10.1128/jcm.34.9.2148-2153.1996 |
| format | Article |
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(University of Guelph, Guelph, Canada.) ; Bannerman, E ; Jemmi, T ; Bille, J</creator><creatorcontrib>Boerlin, P. (University of Guelph, Guelph, Canada.) ; Bannerman, E ; Jemmi, T ; Bille, J</creatorcontrib><description>Macrorestriction analysis by pulsed-field gel electrophoresis was used to assess the diversity of strains within the epidemic-associated electrophoretic type 1 (ET1) clone of Listeria monocytogenes. For this purpose, a total of 144 isolates from Switzerland shown by multilocus enzyme electrophoresis to belong to the ET1 were examined. These isolates were subtyped by macrorestriction analysis using the enzymes ApaI and SmaI and field inversion gel electrophoresis. Among these 144 isolates, 45 were isolated in human listeriosis cases of the postepidemic period of 1988 to 1993 and 44 were isolated in animal listeriosis cases of the same period. Forty-seven isolates were from the epidemic period of 1983 to 1987, and eight additional isolates were from cattle from two different farms. Twenty-nine different subtypes could be identified among the 144 isolates tested. Five major subtypes were found more frequently than the others during the postepidemic period, both in humans and in animals. Two of these subtypes had been previously implicated in outbreaks of listeriosis, thus suggesting that particular pulsed-field gel electrophoresis subtypes may be frequently associated with disease in humans and animals. Two of these frequent subtypes were also suspected to be related to small clusters of listeriosis cases during the postepidemic period. The results obtained by typing epidemiologically related isolates from different animals within the same farms and from different body sites of a given patient confirmed the potential of macrorestriction analysis for epidemiological studies restricted to short periods of time and to small number of isolates. The analysis of 47 isolates related to the Swiss listeriosis epidemic period of 1983 to 1987 and the use of Southern blotting and hybridisation experiments show that the interpretation of relatedness between isolates presenting slightly different macrorestriction patterns may be more complete than commonly accepted. In such cases, c</description><identifier>ISSN: 0095-1137</identifier><identifier>EISSN: 1098-660X</identifier><identifier>DOI: 10.1128/jcm.34.9.2148-2153.1996</identifier><identifier>PMID: 8862575</identifier><identifier>CODEN: JCMIDW</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Animals ; Bacteriology ; Biological and medical sciences ; Cattle ; Disease Outbreaks ; Epidemiology ; Fundamental and applied biological sciences. Psychology ; Humans ; INSOLACION ; INSOLATION ; LISTERIA MONOCYTOGENES ; Listeria monocytogenes - classification ; Listeria monocytogenes - genetics ; Listeria monocytogenes - isolation & purification ; Listeriosis - epidemiology ; Listeriosis - microbiology ; Microbiology ; SUISSE ; SUIZA ; Switzerland - epidemiology</subject><ispartof>Journal of Clinical Microbiology, 1996-09, Vol.34 (9), p.2148-2153</ispartof><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c570t-58ac3e8d66a1704c1ed0f27dc507c635cd08e1ec95d28b4b0a49aca0952fcae43</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC229207/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC229207/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,724,777,781,882,3175,3176,27905,27906,53772,53774</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3185791$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8862575$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Boerlin, P. (University of Guelph, Guelph, Canada.)</creatorcontrib><creatorcontrib>Bannerman, E</creatorcontrib><creatorcontrib>Jemmi, T</creatorcontrib><creatorcontrib>Bille, J</creatorcontrib><title>Subtyping Listeria monocytogenes isolates genetically related to the Swiss epidemic clone</title><title>Journal of Clinical Microbiology</title><addtitle>J Clin Microbiol</addtitle><description>Macrorestriction analysis by pulsed-field gel electrophoresis was used to assess the diversity of strains within the epidemic-associated electrophoretic type 1 (ET1) clone of Listeria monocytogenes. For this purpose, a total of 144 isolates from Switzerland shown by multilocus enzyme electrophoresis to belong to the ET1 were examined. These isolates were subtyped by macrorestriction analysis using the enzymes ApaI and SmaI and field inversion gel electrophoresis. Among these 144 isolates, 45 were isolated in human listeriosis cases of the postepidemic period of 1988 to 1993 and 44 were isolated in animal listeriosis cases of the same period. Forty-seven isolates were from the epidemic period of 1983 to 1987, and eight additional isolates were from cattle from two different farms. Twenty-nine different subtypes could be identified among the 144 isolates tested. Five major subtypes were found more frequently than the others during the postepidemic period, both in humans and in animals. Two of these subtypes had been previously implicated in outbreaks of listeriosis, thus suggesting that particular pulsed-field gel electrophoresis subtypes may be frequently associated with disease in humans and animals. Two of these frequent subtypes were also suspected to be related to small clusters of listeriosis cases during the postepidemic period. The results obtained by typing epidemiologically related isolates from different animals within the same farms and from different body sites of a given patient confirmed the potential of macrorestriction analysis for epidemiological studies restricted to short periods of time and to small number of isolates. The analysis of 47 isolates related to the Swiss listeriosis epidemic period of 1983 to 1987 and the use of Southern blotting and hybridisation experiments show that the interpretation of relatedness between isolates presenting slightly different macrorestriction patterns may be more complete than commonly accepted. In such cases, c</description><subject>Animals</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Cattle</subject><subject>Disease Outbreaks</subject><subject>Epidemiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>INSOLACION</subject><subject>INSOLATION</subject><subject>LISTERIA MONOCYTOGENES</subject><subject>Listeria monocytogenes - classification</subject><subject>Listeria monocytogenes - genetics</subject><subject>Listeria monocytogenes - isolation & purification</subject><subject>Listeriosis - epidemiology</subject><subject>Listeriosis - microbiology</subject><subject>Microbiology</subject><subject>SUISSE</subject><subject>SUIZA</subject><subject>Switzerland - epidemiology</subject><issn>0095-1137</issn><issn>1098-660X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkl2LEzEUhoMoa7f6BwRxBPFuxnxOkgsvZHFVKHhRF_QqpJkzbZaZSU1Sl_57M7QU98qrfLzPe3IObxB6Q3BDCFUf7t3YMN7ohhKuakoEa4jW7RO0IFirum3xz6dogbEWNSFMPkfXKd1jTDgX4gpdKdVSIcUC_VofNvm499O2WvmUIXpbjWEK7pjDFiZIlU9hsLls5mP2zg7DsYow33VVDlXeQbV-8ClVsPcdjN5VbggTvEDPejskeHlel-ju9vOPm6_16vuXbzefVrUTEudaKOsYqK5tLZGYOwId7qnsnMDStUy4Disg4LToqNrwDbZcW2fLYLR3Fjhboo-nuvvDZoTOwZSjHcw--tHGownWm8fK5HdmG_4YSjXFsvjfn_0x_D5Aymb0ycEw2AnCIRmpOFWk_T9IRCs0YayA8gS6GFKK0F-aIdjM6ZmSnmHcaDOnZ-b0zJxecb7-d5aL7xxX0d-ddZtKEH20k_PpgjGihCwtLNHbE7bz292Dj2BsGh8_WphXJ6a3wdhtLGXu1loyWT4J-wsSzLpF</recordid><startdate>19960901</startdate><enddate>19960901</enddate><creator>Boerlin, P. (University of Guelph, Guelph, Canada.)</creator><creator>Bannerman, E</creator><creator>Jemmi, T</creator><creator>Bille, J</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19960901</creationdate><title>Subtyping Listeria monocytogenes isolates genetically related to the Swiss epidemic clone</title><author>Boerlin, P. (University of Guelph, Guelph, Canada.) ; Bannerman, E ; Jemmi, T ; Bille, J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c570t-58ac3e8d66a1704c1ed0f27dc507c635cd08e1ec95d28b4b0a49aca0952fcae43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animals</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Cattle</topic><topic>Disease Outbreaks</topic><topic>Epidemiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>INSOLACION</topic><topic>INSOLATION</topic><topic>LISTERIA MONOCYTOGENES</topic><topic>Listeria monocytogenes - classification</topic><topic>Listeria monocytogenes - genetics</topic><topic>Listeria monocytogenes - isolation & purification</topic><topic>Listeriosis - epidemiology</topic><topic>Listeriosis - microbiology</topic><topic>Microbiology</topic><topic>SUISSE</topic><topic>SUIZA</topic><topic>Switzerland - epidemiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Boerlin, P. (University of Guelph, Guelph, Canada.)</creatorcontrib><creatorcontrib>Bannerman, E</creatorcontrib><creatorcontrib>Jemmi, T</creatorcontrib><creatorcontrib>Bille, J</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Clinical Microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Boerlin, P. (University of Guelph, Guelph, Canada.)</au><au>Bannerman, E</au><au>Jemmi, T</au><au>Bille, J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Subtyping Listeria monocytogenes isolates genetically related to the Swiss epidemic clone</atitle><jtitle>Journal of Clinical Microbiology</jtitle><addtitle>J Clin Microbiol</addtitle><date>1996-09-01</date><risdate>1996</risdate><volume>34</volume><issue>9</issue><spage>2148</spage><epage>2153</epage><pages>2148-2153</pages><issn>0095-1137</issn><eissn>1098-660X</eissn><coden>JCMIDW</coden><abstract>Macrorestriction analysis by pulsed-field gel electrophoresis was used to assess the diversity of strains within the epidemic-associated electrophoretic type 1 (ET1) clone of Listeria monocytogenes. For this purpose, a total of 144 isolates from Switzerland shown by multilocus enzyme electrophoresis to belong to the ET1 were examined. These isolates were subtyped by macrorestriction analysis using the enzymes ApaI and SmaI and field inversion gel electrophoresis. Among these 144 isolates, 45 were isolated in human listeriosis cases of the postepidemic period of 1988 to 1993 and 44 were isolated in animal listeriosis cases of the same period. Forty-seven isolates were from the epidemic period of 1983 to 1987, and eight additional isolates were from cattle from two different farms. Twenty-nine different subtypes could be identified among the 144 isolates tested. Five major subtypes were found more frequently than the others during the postepidemic period, both in humans and in animals. Two of these subtypes had been previously implicated in outbreaks of listeriosis, thus suggesting that particular pulsed-field gel electrophoresis subtypes may be frequently associated with disease in humans and animals. Two of these frequent subtypes were also suspected to be related to small clusters of listeriosis cases during the postepidemic period. The results obtained by typing epidemiologically related isolates from different animals within the same farms and from different body sites of a given patient confirmed the potential of macrorestriction analysis for epidemiological studies restricted to short periods of time and to small number of isolates. The analysis of 47 isolates related to the Swiss listeriosis epidemic period of 1983 to 1987 and the use of Southern blotting and hybridisation experiments show that the interpretation of relatedness between isolates presenting slightly different macrorestriction patterns may be more complete than commonly accepted. In such cases, c</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>8862575</pmid><doi>10.1128/jcm.34.9.2148-2153.1996</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | American Society for Microbiology; MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Animals Bacteriology Biological and medical sciences Cattle Disease Outbreaks Epidemiology Fundamental and applied biological sciences. Psychology Humans INSOLACION INSOLATION LISTERIA MONOCYTOGENES Listeria monocytogenes - classification Listeria monocytogenes - genetics Listeria monocytogenes - isolation & purification Listeriosis - epidemiology Listeriosis - microbiology Microbiology SUISSE SUIZA Switzerland - epidemiology |
| title | Subtyping Listeria monocytogenes isolates genetically related to the Swiss epidemic clone |
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