P149-M Proteomics Sample Preparation Using Magnetic Bead Based Technologies
We have developed a range of magnetic beads for protein and/or peptide sample isolation and fractionation that can be utilised in proteomics strategies and workflows. A significant challenge in proteomics today is overcoming the dynamic range of protein abundance. High throughput proteomics requires...
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| Veröffentlicht in: | Journal of biomolecular techniques 2007-02, Vol.18 (1), p.52-52 |
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| container_title | Journal of biomolecular techniques |
| container_volume | 18 |
| creator | Bergholtz, S. Breivold, E. Tran, D. Ragnhildstveit, E. Brekke, O. Evensen, K. Fonnum, G. Bosnes, M. Gillooly, D. |
| description | We have developed a range of magnetic beads for protein and/or peptide sample isolation and fractionation that can be utilised in proteomics strategies and workflows.
A significant challenge in proteomics today is overcoming the dynamic range of protein abundance. High throughput proteomics requires efficient methods to analyze complex protein mixtures. It is often necessary to reduce sample complexity for many proteomics strategies. The use of magnetic beads for sample preparation enables protocols to be automated and throughput to be increased. The kinetics of bead-based sample preparation is very efficient and washing can be done thoroughly. In addition, preparation is flexible regarding sample and buffer volumes. Consequently, low abundant proteins can be concentrated from large sample volumes.
We have developed protein purification methods based on the use of magnetic bead technology. These methods are used for the fractionation of complex protein samples and for isolation of proteins or peptides. |
| format | Article |
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A significant challenge in proteomics today is overcoming the dynamic range of protein abundance. High throughput proteomics requires efficient methods to analyze complex protein mixtures. It is often necessary to reduce sample complexity for many proteomics strategies. The use of magnetic beads for sample preparation enables protocols to be automated and throughput to be increased. The kinetics of bead-based sample preparation is very efficient and washing can be done thoroughly. In addition, preparation is flexible regarding sample and buffer volumes. Consequently, low abundant proteins can be concentrated from large sample volumes.
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A significant challenge in proteomics today is overcoming the dynamic range of protein abundance. High throughput proteomics requires efficient methods to analyze complex protein mixtures. It is often necessary to reduce sample complexity for many proteomics strategies. The use of magnetic beads for sample preparation enables protocols to be automated and throughput to be increased. The kinetics of bead-based sample preparation is very efficient and washing can be done thoroughly. In addition, preparation is flexible regarding sample and buffer volumes. Consequently, low abundant proteins can be concentrated from large sample volumes.
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A significant challenge in proteomics today is overcoming the dynamic range of protein abundance. High throughput proteomics requires efficient methods to analyze complex protein mixtures. It is often necessary to reduce sample complexity for many proteomics strategies. The use of magnetic beads for sample preparation enables protocols to be automated and throughput to be increased. The kinetics of bead-based sample preparation is very efficient and washing can be done thoroughly. In addition, preparation is flexible regarding sample and buffer volumes. Consequently, low abundant proteins can be concentrated from large sample volumes.
We have developed protein purification methods based on the use of magnetic bead technology. These methods are used for the fractionation of complex protein samples and for isolation of proteins or peptides.</abstract><pub>The Association of Biomolecular Resource Facilities</pub></addata></record> |
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| identifier | ISSN: 1524-0215 |
| ispartof | Journal of biomolecular techniques, 2007-02, Vol.18 (1), p.52-52 |
| issn | 1524-0215 |
| language | eng |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Poster Abstracts: Proteomics |
| title | P149-M Proteomics Sample Preparation Using Magnetic Bead Based Technologies |
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