Inactivation of cleavage factor I components Rna14p and Rna15p induces sequestration of small nucleolar ribonucleoproteins at discrete sites in the nucleus
Small nucleolar RNAs (snoRNAs) associate with specific proteins forming small nucleolar ribonucleoprotein (snoRNP) particles, which are essential for ribosome biogenesis. The snoRNAs are transcribed, processed, and assembled in snoRNPs in the nucleoplasm. Mature particles are then transported to the...
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| Veröffentlicht in: | Molecular biology of the cell 2008-04, Vol.19 (4), p.1499-1508 |
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| creator | Carneiro, Tiago Carvalho, Célia Braga, José Rino, José Milligan, Laura Tollervey, David Carmo-Fonseca, Maria |
| description | Small nucleolar RNAs (snoRNAs) associate with specific proteins forming small nucleolar ribonucleoprotein (snoRNP) particles, which are essential for ribosome biogenesis. The snoRNAs are transcribed, processed, and assembled in snoRNPs in the nucleoplasm. Mature particles are then transported to the nucleolus. In yeast, 3'-end maturation of snoRNAs involves the activity of Rnt1p endonuclease and cleavage factor IA (CFIA). We report that after inhibition of CFIA components Rna14p and Rna15p, the snoRNP proteins Nop1p, Nop58p, and Gar1p delocalize from the nucleolus and accumulate in discrete nucleoplasmic foci. The U14 snoRNA, but not U3 snoRNA, similarly redistributes from the nucleolus to the nucleoplasmic foci. Simultaneous depletion of either Rna14p or Rna15p and the nuclear exosome component Rrp6p induces accumulation of poly(A)(+) RNA at the snoRNP-containing foci. We propose that the foci detected after CFIA inactivation correspond to quality control centers in the nucleoplasm. |
| doi_str_mv | 10.1091/mbc.E07-10-1015 |
| format | Article |
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The snoRNAs are transcribed, processed, and assembled in snoRNPs in the nucleoplasm. Mature particles are then transported to the nucleolus. In yeast, 3'-end maturation of snoRNAs involves the activity of Rnt1p endonuclease and cleavage factor IA (CFIA). We report that after inhibition of CFIA components Rna14p and Rna15p, the snoRNP proteins Nop1p, Nop58p, and Gar1p delocalize from the nucleolus and accumulate in discrete nucleoplasmic foci. The U14 snoRNA, but not U3 snoRNA, similarly redistributes from the nucleolus to the nucleoplasmic foci. Simultaneous depletion of either Rna14p or Rna15p and the nuclear exosome component Rrp6p induces accumulation of poly(A)(+) RNA at the snoRNP-containing foci. 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We propose that the foci detected after CFIA inactivation correspond to quality control centers in the nucleoplasm.</description><subject>Base Sequence</subject><subject>Cell Nucleus - metabolism</subject><subject>Genes, Fungal</subject><subject>Green Fluorescent Proteins - genetics</subject><subject>Green Fluorescent Proteins - metabolism</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>mRNA Cleavage and Polyadenylation Factors - antagonists & inhibitors</subject><subject>mRNA Cleavage and Polyadenylation Factors - genetics</subject><subject>mRNA Cleavage and Polyadenylation Factors - metabolism</subject><subject>Mutation</subject><subject>Nuclear Proteins - genetics</subject><subject>Nuclear Proteins - metabolism</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Recombinant Fusion Proteins - metabolism</subject><subject>Ribonucleoproteins, Small Nucleolar - genetics</subject><subject>Ribonucleoproteins, Small Nucleolar - metabolism</subject><subject>RNA, Fungal - genetics</subject><subject>RNA, Fungal - metabolism</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae - metabolism</subject><subject>Saccharomyces cerevisiae Proteins - antagonists & inhibitors</subject><subject>Saccharomyces cerevisiae Proteins - genetics</subject><subject>Saccharomyces cerevisiae Proteins - metabolism</subject><issn>1059-1524</issn><issn>1939-4586</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkU9rFjEQxoMotlbP3iQnb9sm2Wz-XAQpVV8oCKLnkGRn28husibZF_wsfllj96UqBDKTPPObTB6EXlNySYmmV4vzlzdEdpS0RYcn6JzqXnd8UOJpi8mgOzowfoZelPKdEMq5kM_RGVWs56pX5-jXIVpfw9HWkCJOE_Yz2KO9Azy185TxAfu0rClCrAV_iZbyFds4PoTDikMcNw8FF_ixQan5kVMWO884bo2XZptxDi7t2ZpThRALthWPofgMFXAJtVFCxPUe9qqtvETPJjsXeHXaL9C3Dzdfrz91t58_Hq7f33aeC1U732urhCCDcEzSfuqJ01ZwqwnnE3BiNZ8olYKDG72S4KiXTAlFPHGKMddfoHc7d93cAqNvo2Y7mzWHxeafJtlg_r-J4d7cpaNhTFNOaAO8PQFyevgGs7S5YJ5thLQVIwmXkjPRhFe70OdUSobpsQkl5o-hphlqgMg9p0OrePPv2_7qTw72vwHIsaDZ</recordid><startdate>200804</startdate><enddate>200804</enddate><creator>Carneiro, Tiago</creator><creator>Carvalho, Célia</creator><creator>Braga, José</creator><creator>Rino, José</creator><creator>Milligan, Laura</creator><creator>Tollervey, David</creator><creator>Carmo-Fonseca, Maria</creator><general>The American Society for Cell Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>200804</creationdate><title>Inactivation of cleavage factor I components Rna14p and Rna15p induces sequestration of small nucleolar ribonucleoproteins at discrete sites in the nucleus</title><author>Carneiro, Tiago ; 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The snoRNAs are transcribed, processed, and assembled in snoRNPs in the nucleoplasm. Mature particles are then transported to the nucleolus. In yeast, 3'-end maturation of snoRNAs involves the activity of Rnt1p endonuclease and cleavage factor IA (CFIA). We report that after inhibition of CFIA components Rna14p and Rna15p, the snoRNP proteins Nop1p, Nop58p, and Gar1p delocalize from the nucleolus and accumulate in discrete nucleoplasmic foci. The U14 snoRNA, but not U3 snoRNA, similarly redistributes from the nucleolus to the nucleoplasmic foci. Simultaneous depletion of either Rna14p or Rna15p and the nuclear exosome component Rrp6p induces accumulation of poly(A)(+) RNA at the snoRNP-containing foci. 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| subjects | Base Sequence Cell Nucleus - metabolism Genes, Fungal Green Fluorescent Proteins - genetics Green Fluorescent Proteins - metabolism In Situ Hybridization, Fluorescence mRNA Cleavage and Polyadenylation Factors - antagonists & inhibitors mRNA Cleavage and Polyadenylation Factors - genetics mRNA Cleavage and Polyadenylation Factors - metabolism Mutation Nuclear Proteins - genetics Nuclear Proteins - metabolism Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism Ribonucleoproteins, Small Nucleolar - genetics Ribonucleoproteins, Small Nucleolar - metabolism RNA, Fungal - genetics RNA, Fungal - metabolism RNA, Messenger - genetics RNA, Messenger - metabolism Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism Saccharomyces cerevisiae Proteins - antagonists & inhibitors Saccharomyces cerevisiae Proteins - genetics Saccharomyces cerevisiae Proteins - metabolism |
| title | Inactivation of cleavage factor I components Rna14p and Rna15p induces sequestration of small nucleolar ribonucleoproteins at discrete sites in the nucleus |
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