The contribution of intracellular calcium stores to mEPSCs recorded in layer II neurones of rat barrel cortex
Loading slices of rat barrel cortex with 50 μ m BAPTA-AM while recording from pyramidal cells in layer II induces a marked reduction in both the frequency and amplitudes of mEPSCs. These changes are due to a presynaptic action. Blocking the refilling of Ca 2+ stores with 20 μ m cyclopiazonic acid...
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| Veröffentlicht in: | The Journal of physiology 2002-12, Vol.545 (2), p.521-535 |
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| creator | Simkus, Christopher R. L. Stricker, Christian |
| description | Loading slices of rat barrel cortex with 50 μ m BAPTA-AM while recording from pyramidal cells in layer II induces a marked reduction in both the frequency and amplitudes
of mEPSCs. These changes are due to a presynaptic action. Blocking the refilling of Ca 2+ stores with 20 μ m cyclopiazonic acid (CPA), a SERCA pump inhibitor, in conjunction with neuronal depolarisation to activate Ca 2+ stores, results in a similar reduction of mEPSCs to that observed with BAPTA-AM, indicating that the source for intracellular
Ca 2+ is the endoplasmic reticulum. Block or activation of ryanodine receptors by 20 μ m ryanodine or 10 m m caffeine, respectively, shows that a significant proportion of mEPSCs are caused by Ca 2+ release from ryanodine stores. Blocking IP 3 receptors with 14 μ m 2-aminoethoxydiphenylborane (2APB) also reduces the frequency and amplitude of mEPSCs, indicating the involvement of IP 3 stores in the generation of mEPSCs. Activation of group I metabotropic receptors with 20 μ m ( RS) -3,5-dihydroxyphenylglycine (DHPG) results in a significant increase in the frequency of mEPSCs, further supporting the role
of IP 3 receptors and indicating a role of group I metabotropic receptors in causing transmitter release. Statistical evidence is
presented for Ca 2+ -induced Ca 2+ release (CICR) from ryanodine stores after the spontaneous opening of IP 3 stores. |
| doi_str_mv | 10.1113/jphysiol.2002.022103 |
| format | Article |
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of mEPSCs. These changes are due to a presynaptic action. Blocking the refilling of Ca 2+ stores with 20 μ m cyclopiazonic acid (CPA), a SERCA pump inhibitor, in conjunction with neuronal depolarisation to activate Ca 2+ stores, results in a similar reduction of mEPSCs to that observed with BAPTA-AM, indicating that the source for intracellular
Ca 2+ is the endoplasmic reticulum. Block or activation of ryanodine receptors by 20 μ m ryanodine or 10 m m caffeine, respectively, shows that a significant proportion of mEPSCs are caused by Ca 2+ release from ryanodine stores. Blocking IP 3 receptors with 14 μ m 2-aminoethoxydiphenylborane (2APB) also reduces the frequency and amplitude of mEPSCs, indicating the involvement of IP 3 stores in the generation of mEPSCs. Activation of group I metabotropic receptors with 20 μ m ( RS) -3,5-dihydroxyphenylglycine (DHPG) results in a significant increase in the frequency of mEPSCs, further supporting the role
of IP 3 receptors and indicating a role of group I metabotropic receptors in causing transmitter release. Statistical evidence is
presented for Ca 2+ -induced Ca 2+ release (CICR) from ryanodine stores after the spontaneous opening of IP 3 stores.</description><identifier>ISSN: 0022-3751</identifier><identifier>EISSN: 1469-7793</identifier><identifier>DOI: 10.1113/jphysiol.2002.022103</identifier><identifier>PMID: 12456831</identifier><language>eng</language><publisher>Oxford, UK: The Physiological Society</publisher><subject>Animals ; Caffeine - pharmacology ; Calcium - physiology ; Calcium Channel Agonists - pharmacology ; Calcium Channel Blockers - pharmacology ; Calcium-Transporting ATPases - antagonists & inhibitors ; Calcium-Transporting ATPases - metabolism ; Central Nervous System Stimulants - pharmacology ; Chelating Agents - pharmacology ; Egtazic Acid - analogs & derivatives ; Electrophysiology ; Endoplasmic Reticulum - drug effects ; Endoplasmic Reticulum - metabolism ; Excitatory Postsynaptic Potentials - physiology ; In Vitro Techniques ; Inosine Triphosphate - pharmacology ; Iontophoresis ; Neurons - physiology ; Original ; Patch-Clamp Techniques ; Rats ; Rats, Wistar ; Receptors, Presynaptic - drug effects ; Ryanodine Receptor Calcium Release Channel - drug effects ; Ryanodine Receptor Calcium Release Channel - metabolism ; Sarcoplasmic Reticulum Calcium-Transporting ATPases ; Somatosensory Cortex - cytology ; Somatosensory Cortex - physiology</subject><ispartof>The Journal of physiology, 2002-12, Vol.545 (2), p.521-535</ispartof><rights>2002 The Journal of Physiology © 2002 The Physiological Society</rights><rights>The Physiological Society 2002 2002</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5841-4b4885af7a44105ded51085ad99c01227d5393783c2d16a84d06e303cddf74b73</citedby><cites>FETCH-LOGICAL-c5841-4b4885af7a44105ded51085ad99c01227d5393783c2d16a84d06e303cddf74b73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2290677/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2290677/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,1411,1427,27903,27904,45553,45554,46388,46812,53770,53772</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12456831$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Simkus, Christopher R. L.</creatorcontrib><creatorcontrib>Stricker, Christian</creatorcontrib><title>The contribution of intracellular calcium stores to mEPSCs recorded in layer II neurones of rat barrel cortex</title><title>The Journal of physiology</title><addtitle>J Physiol</addtitle><description>Loading slices of rat barrel cortex with 50 μ m BAPTA-AM while recording from pyramidal cells in layer II induces a marked reduction in both the frequency and amplitudes
of mEPSCs. These changes are due to a presynaptic action. Blocking the refilling of Ca 2+ stores with 20 μ m cyclopiazonic acid (CPA), a SERCA pump inhibitor, in conjunction with neuronal depolarisation to activate Ca 2+ stores, results in a similar reduction of mEPSCs to that observed with BAPTA-AM, indicating that the source for intracellular
Ca 2+ is the endoplasmic reticulum. Block or activation of ryanodine receptors by 20 μ m ryanodine or 10 m m caffeine, respectively, shows that a significant proportion of mEPSCs are caused by Ca 2+ release from ryanodine stores. Blocking IP 3 receptors with 14 μ m 2-aminoethoxydiphenylborane (2APB) also reduces the frequency and amplitude of mEPSCs, indicating the involvement of IP 3 stores in the generation of mEPSCs. Activation of group I metabotropic receptors with 20 μ m ( RS) -3,5-dihydroxyphenylglycine (DHPG) results in a significant increase in the frequency of mEPSCs, further supporting the role
of IP 3 receptors and indicating a role of group I metabotropic receptors in causing transmitter release. Statistical evidence is
presented for Ca 2+ -induced Ca 2+ release (CICR) from ryanodine stores after the spontaneous opening of IP 3 stores.</description><subject>Animals</subject><subject>Caffeine - pharmacology</subject><subject>Calcium - physiology</subject><subject>Calcium Channel Agonists - pharmacology</subject><subject>Calcium Channel Blockers - pharmacology</subject><subject>Calcium-Transporting ATPases - antagonists & inhibitors</subject><subject>Calcium-Transporting ATPases - metabolism</subject><subject>Central Nervous System Stimulants - pharmacology</subject><subject>Chelating Agents - pharmacology</subject><subject>Egtazic Acid - analogs & derivatives</subject><subject>Electrophysiology</subject><subject>Endoplasmic Reticulum - drug effects</subject><subject>Endoplasmic Reticulum - metabolism</subject><subject>Excitatory Postsynaptic Potentials - physiology</subject><subject>In Vitro Techniques</subject><subject>Inosine Triphosphate - pharmacology</subject><subject>Iontophoresis</subject><subject>Neurons - physiology</subject><subject>Original</subject><subject>Patch-Clamp Techniques</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Receptors, Presynaptic - drug effects</subject><subject>Ryanodine Receptor Calcium Release Channel - drug effects</subject><subject>Ryanodine Receptor Calcium Release Channel - metabolism</subject><subject>Sarcoplasmic Reticulum Calcium-Transporting ATPases</subject><subject>Somatosensory Cortex - cytology</subject><subject>Somatosensory Cortex - physiology</subject><issn>0022-3751</issn><issn>1469-7793</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUtv1DAUhS0EotPCP0DIK-gmg59xskGqRgUGVaISw9pybKdx5cSDnVDy73GU4bVBrCz7fufce30AeIHRFmNM39wfuzm54LcEIbJFhGBEH4ENZmVdCFHTx2CTC6SgguMzcJ7SPUKYorp-Cs4wYbysKN6A_tBZqMMwRtdMowsDDC10-aq09X7yKkKtvHZTD9MYok1wDLC_vv28SzBaHaKxJvPQq9lGuN_DwU4xDJnLPlGNsFExWp9bxNF-fwaetMon-_x0XoAv764Puw_Fzaf3-93VTaF5xXDBGlZVXLVCMYYRzy04RvnB1LVGmBBhOK2pqKgmBpeqYgaVliKqjWkFawS9AG9X3-PU9NZouyzk5TG6XsVZBuXk35XBdfIufJOE1KgUi8Grk0EMXyebRtm7tPyIGmyYkhRE0JKUKIOX_wSxqBBHHHOWUbaiOoaUom1_zYORXCKVPyOVS6RyjTTLXv65y2_RKcMMVCvw4Lyd_8tUHj7ecrJIX6_Szt11Dy5aucIpaGfHWXLGJZEL-QN_O8BC</recordid><startdate>200212</startdate><enddate>200212</enddate><creator>Simkus, Christopher R. L.</creator><creator>Stricker, Christian</creator><general>The Physiological Society</general><general>Blackwell Publishing Ltd</general><general>Blackwell Science Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7TK</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>200212</creationdate><title>The contribution of intracellular calcium stores to mEPSCs recorded in layer II neurones of rat barrel cortex</title><author>Simkus, Christopher R. L. ; Stricker, Christian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5841-4b4885af7a44105ded51085ad99c01227d5393783c2d16a84d06e303cddf74b73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Caffeine - pharmacology</topic><topic>Calcium - physiology</topic><topic>Calcium Channel Agonists - pharmacology</topic><topic>Calcium Channel Blockers - pharmacology</topic><topic>Calcium-Transporting ATPases - antagonists & inhibitors</topic><topic>Calcium-Transporting ATPases - metabolism</topic><topic>Central Nervous System Stimulants - pharmacology</topic><topic>Chelating Agents - pharmacology</topic><topic>Egtazic Acid - analogs & derivatives</topic><topic>Electrophysiology</topic><topic>Endoplasmic Reticulum - drug effects</topic><topic>Endoplasmic Reticulum - metabolism</topic><topic>Excitatory Postsynaptic Potentials - physiology</topic><topic>In Vitro Techniques</topic><topic>Inosine Triphosphate - pharmacology</topic><topic>Iontophoresis</topic><topic>Neurons - physiology</topic><topic>Original</topic><topic>Patch-Clamp Techniques</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Receptors, Presynaptic - drug effects</topic><topic>Ryanodine Receptor Calcium Release Channel - drug effects</topic><topic>Ryanodine Receptor Calcium Release Channel - metabolism</topic><topic>Sarcoplasmic Reticulum Calcium-Transporting ATPases</topic><topic>Somatosensory Cortex - cytology</topic><topic>Somatosensory Cortex - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Simkus, Christopher R. L.</creatorcontrib><creatorcontrib>Stricker, Christian</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Simkus, Christopher R. L.</au><au>Stricker, Christian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The contribution of intracellular calcium stores to mEPSCs recorded in layer II neurones of rat barrel cortex</atitle><jtitle>The Journal of physiology</jtitle><addtitle>J Physiol</addtitle><date>2002-12</date><risdate>2002</risdate><volume>545</volume><issue>2</issue><spage>521</spage><epage>535</epage><pages>521-535</pages><issn>0022-3751</issn><eissn>1469-7793</eissn><abstract>Loading slices of rat barrel cortex with 50 μ m BAPTA-AM while recording from pyramidal cells in layer II induces a marked reduction in both the frequency and amplitudes
of mEPSCs. These changes are due to a presynaptic action. Blocking the refilling of Ca 2+ stores with 20 μ m cyclopiazonic acid (CPA), a SERCA pump inhibitor, in conjunction with neuronal depolarisation to activate Ca 2+ stores, results in a similar reduction of mEPSCs to that observed with BAPTA-AM, indicating that the source for intracellular
Ca 2+ is the endoplasmic reticulum. Block or activation of ryanodine receptors by 20 μ m ryanodine or 10 m m caffeine, respectively, shows that a significant proportion of mEPSCs are caused by Ca 2+ release from ryanodine stores. Blocking IP 3 receptors with 14 μ m 2-aminoethoxydiphenylborane (2APB) also reduces the frequency and amplitude of mEPSCs, indicating the involvement of IP 3 stores in the generation of mEPSCs. Activation of group I metabotropic receptors with 20 μ m ( RS) -3,5-dihydroxyphenylglycine (DHPG) results in a significant increase in the frequency of mEPSCs, further supporting the role
of IP 3 receptors and indicating a role of group I metabotropic receptors in causing transmitter release. Statistical evidence is
presented for Ca 2+ -induced Ca 2+ release (CICR) from ryanodine stores after the spontaneous opening of IP 3 stores.</abstract><cop>Oxford, UK</cop><pub>The Physiological Society</pub><pmid>12456831</pmid><doi>10.1113/jphysiol.2002.022103</doi><tpages>15</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Caffeine - pharmacology Calcium - physiology Calcium Channel Agonists - pharmacology Calcium Channel Blockers - pharmacology Calcium-Transporting ATPases - antagonists & inhibitors Calcium-Transporting ATPases - metabolism Central Nervous System Stimulants - pharmacology Chelating Agents - pharmacology Egtazic Acid - analogs & derivatives Electrophysiology Endoplasmic Reticulum - drug effects Endoplasmic Reticulum - metabolism Excitatory Postsynaptic Potentials - physiology In Vitro Techniques Inosine Triphosphate - pharmacology Iontophoresis Neurons - physiology Original Patch-Clamp Techniques Rats Rats, Wistar Receptors, Presynaptic - drug effects Ryanodine Receptor Calcium Release Channel - drug effects Ryanodine Receptor Calcium Release Channel - metabolism Sarcoplasmic Reticulum Calcium-Transporting ATPases Somatosensory Cortex - cytology Somatosensory Cortex - physiology |
| title | The contribution of intracellular calcium stores to mEPSCs recorded in layer II neurones of rat barrel cortex |
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