Physical mobilization of secretory vesicles facilitates neuropeptide release by nerve growth factor-differentiated PC12 Cells
It has been speculated that neurosecretion can be enhanced by increasing the motion, and hence, the availability of cytoplasmic secretory vesicles. However, facilitator-induced physical mobilization of secretory vesicles has not been observed directly in living cells, and recent experimental results...
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| Veröffentlicht in: | The Journal of physiology 2002-07, Vol.542 (2), p.395-402 |
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| container_title | The Journal of physiology |
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| creator | Ng, Yuen‐Keng Lu, Xinghua Levitan, Edwin S. |
| description | It has been speculated that neurosecretion can be enhanced by increasing the motion, and hence, the availability of cytoplasmic
secretory vesicles. However, facilitator-induced physical mobilization of secretory vesicles has not been observed directly
in living cells, and recent experimental results call this hypothesis into question. Here, high resolution green fluorescent
protein (GFP)-based measurements in nerve growth factor-differentiated PC12 cells are used to test whether altering dense
core vesicle (DCV) motion affects neuropeptide release. Experiments with mycalolide B and jasplakinolide demonstrate that
neuropeptidergic DCV motion at the ends of processes is proportional to F-actin. Furthermore, Ba 2+ increases DCV mobility without detectably modifying F-actin. Finally, we show that altering DCV motion by changing F-actin
or stimulating with Ba 2+ proportionally changes sustained neuropeptide release. Therefore, increasing DCV mobility facilitates prolonged neuropeptide
release. |
| doi_str_mv | 10.1113/jphysiol.2002.021733 |
| format | Article |
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secretory vesicles. However, facilitator-induced physical mobilization of secretory vesicles has not been observed directly
in living cells, and recent experimental results call this hypothesis into question. Here, high resolution green fluorescent
protein (GFP)-based measurements in nerve growth factor-differentiated PC12 cells are used to test whether altering dense
core vesicle (DCV) motion affects neuropeptide release. Experiments with mycalolide B and jasplakinolide demonstrate that
neuropeptidergic DCV motion at the ends of processes is proportional to F-actin. Furthermore, Ba 2+ increases DCV mobility without detectably modifying F-actin. Finally, we show that altering DCV motion by changing F-actin
or stimulating with Ba 2+ proportionally changes sustained neuropeptide release. Therefore, increasing DCV mobility facilitates prolonged neuropeptide
release.</description><identifier>ISSN: 0022-3751</identifier><identifier>EISSN: 1469-7793</identifier><identifier>DOI: 10.1113/jphysiol.2002.021733</identifier><identifier>PMID: 12122140</identifier><language>eng</language><publisher>Oxford, UK: The Physiological Society</publisher><subject>Actins - drug effects ; Actins - physiology ; Animals ; Antineoplastic Agents - pharmacology ; Cell Differentiation - drug effects ; Depsipeptides ; Genes, Reporter ; Green Fluorescent Proteins ; Luminescent Proteins - metabolism ; Movement ; Nerve Growth Factor - pharmacology ; Neuropeptides - metabolism ; Original ; Oxazoles - pharmacology ; PC12 Cells ; Peptides, Cyclic - pharmacology ; Pheochromocytoma ; Rats ; Recombinant Proteins - metabolism ; Secretory Vesicles - drug effects ; Secretory Vesicles - physiology ; Secretory Vesicles - ultrastructure</subject><ispartof>The Journal of physiology, 2002-07, Vol.542 (2), p.395-402</ispartof><rights>2002 The Journal of Physiology © 2002 The Physiological Society</rights><rights>The Physiological Society 2002 2002</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4005-cc0bd9c6f1a0424878bb1cd8ab3345208103de7ac61793b44252710948409b213</citedby><cites>FETCH-LOGICAL-c4005-cc0bd9c6f1a0424878bb1cd8ab3345208103de7ac61793b44252710948409b213</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2290425/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2290425/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,1411,1427,27903,27904,45553,45554,46387,46811,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12122140$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ng, Yuen‐Keng</creatorcontrib><creatorcontrib>Lu, Xinghua</creatorcontrib><creatorcontrib>Levitan, Edwin S.</creatorcontrib><title>Physical mobilization of secretory vesicles facilitates neuropeptide release by nerve growth factor-differentiated PC12 Cells</title><title>The Journal of physiology</title><addtitle>J Physiol</addtitle><description>It has been speculated that neurosecretion can be enhanced by increasing the motion, and hence, the availability of cytoplasmic
secretory vesicles. However, facilitator-induced physical mobilization of secretory vesicles has not been observed directly
in living cells, and recent experimental results call this hypothesis into question. Here, high resolution green fluorescent
protein (GFP)-based measurements in nerve growth factor-differentiated PC12 cells are used to test whether altering dense
core vesicle (DCV) motion affects neuropeptide release. Experiments with mycalolide B and jasplakinolide demonstrate that
neuropeptidergic DCV motion at the ends of processes is proportional to F-actin. Furthermore, Ba 2+ increases DCV mobility without detectably modifying F-actin. Finally, we show that altering DCV motion by changing F-actin
or stimulating with Ba 2+ proportionally changes sustained neuropeptide release. Therefore, increasing DCV mobility facilitates prolonged neuropeptide
release.</description><subject>Actins - drug effects</subject><subject>Actins - physiology</subject><subject>Animals</subject><subject>Antineoplastic Agents - pharmacology</subject><subject>Cell Differentiation - drug effects</subject><subject>Depsipeptides</subject><subject>Genes, Reporter</subject><subject>Green Fluorescent Proteins</subject><subject>Luminescent Proteins - metabolism</subject><subject>Movement</subject><subject>Nerve Growth Factor - pharmacology</subject><subject>Neuropeptides - metabolism</subject><subject>Original</subject><subject>Oxazoles - pharmacology</subject><subject>PC12 Cells</subject><subject>Peptides, Cyclic - pharmacology</subject><subject>Pheochromocytoma</subject><subject>Rats</subject><subject>Recombinant Proteins - metabolism</subject><subject>Secretory Vesicles - drug effects</subject><subject>Secretory Vesicles - physiology</subject><subject>Secretory Vesicles - ultrastructure</subject><issn>0022-3751</issn><issn>1469-7793</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc2O0zAUhSMEYsrAGyDkFaxSfG2niTdIqIIBNBJdDGvLcW4aj9w42GmrIPHu4yjlb8fKlu93zj3WybKXQNcAwN_eD90UrXdrRilbUwYl54-yFYiNzMtS8sfZKg1YzssCrrJnMd5TCpxK-TS7AgaMgaCr7OdudjHakYOvrbM_9Gh9T3xLIpqAow8TOWEiHEbSapOQUY_p3uMx-AGH0TZIAjrUEUk9pfdwQrIP_jx2syA55I1tWwzYjzZJG7LbAiNbdC4-z5602kV8cTmvs28fP9xtP-W3X28-b9_f5kZQWuTG0LqRZtOCpoKJqqzqGkxT6ZpzUTBaAeUNltpsIH28FoIVrAQqRSWorBnw6-zd4jsc6wM2JkUJ2qkh2IMOk_Laqn8nve3U3p8UYzJtLJLB64tB8N-PGEd1sNGkL-ge_TGqEiSboyVQLKAJPsaA7e8lQNXcm_rVm5p7U0tvSfbq74B_RJeiElAtwNk6nP7LVN192XE5Z3-zSDu77842oFrg6I3FcVKFYIqpmXwAt2W5IQ</recordid><startdate>20020715</startdate><enddate>20020715</enddate><creator>Ng, Yuen‐Keng</creator><creator>Lu, Xinghua</creator><creator>Levitan, Edwin S.</creator><general>The Physiological Society</general><general>Blackwell Publishing Ltd</general><general>Blackwell Science Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20020715</creationdate><title>Physical mobilization of secretory vesicles facilitates neuropeptide release by nerve growth factor-differentiated PC12 Cells</title><author>Ng, Yuen‐Keng ; Lu, Xinghua ; Levitan, Edwin S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4005-cc0bd9c6f1a0424878bb1cd8ab3345208103de7ac61793b44252710948409b213</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Actins - drug effects</topic><topic>Actins - physiology</topic><topic>Animals</topic><topic>Antineoplastic Agents - pharmacology</topic><topic>Cell Differentiation - drug effects</topic><topic>Depsipeptides</topic><topic>Genes, Reporter</topic><topic>Green Fluorescent Proteins</topic><topic>Luminescent Proteins - metabolism</topic><topic>Movement</topic><topic>Nerve Growth Factor - pharmacology</topic><topic>Neuropeptides - metabolism</topic><topic>Original</topic><topic>Oxazoles - pharmacology</topic><topic>PC12 Cells</topic><topic>Peptides, Cyclic - pharmacology</topic><topic>Pheochromocytoma</topic><topic>Rats</topic><topic>Recombinant Proteins - metabolism</topic><topic>Secretory Vesicles - drug effects</topic><topic>Secretory Vesicles - physiology</topic><topic>Secretory Vesicles - ultrastructure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ng, Yuen‐Keng</creatorcontrib><creatorcontrib>Lu, Xinghua</creatorcontrib><creatorcontrib>Levitan, Edwin S.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ng, Yuen‐Keng</au><au>Lu, Xinghua</au><au>Levitan, Edwin S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Physical mobilization of secretory vesicles facilitates neuropeptide release by nerve growth factor-differentiated PC12 Cells</atitle><jtitle>The Journal of physiology</jtitle><addtitle>J Physiol</addtitle><date>2002-07-15</date><risdate>2002</risdate><volume>542</volume><issue>2</issue><spage>395</spage><epage>402</epage><pages>395-402</pages><issn>0022-3751</issn><eissn>1469-7793</eissn><abstract>It has been speculated that neurosecretion can be enhanced by increasing the motion, and hence, the availability of cytoplasmic
secretory vesicles. However, facilitator-induced physical mobilization of secretory vesicles has not been observed directly
in living cells, and recent experimental results call this hypothesis into question. Here, high resolution green fluorescent
protein (GFP)-based measurements in nerve growth factor-differentiated PC12 cells are used to test whether altering dense
core vesicle (DCV) motion affects neuropeptide release. Experiments with mycalolide B and jasplakinolide demonstrate that
neuropeptidergic DCV motion at the ends of processes is proportional to F-actin. Furthermore, Ba 2+ increases DCV mobility without detectably modifying F-actin. Finally, we show that altering DCV motion by changing F-actin
or stimulating with Ba 2+ proportionally changes sustained neuropeptide release. Therefore, increasing DCV mobility facilitates prolonged neuropeptide
release.</abstract><cop>Oxford, UK</cop><pub>The Physiological Society</pub><pmid>12122140</pmid><doi>10.1113/jphysiol.2002.021733</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Wiley Free Content; IngentaConnect Free/Open Access Journals; PubMed Central |
| subjects | Actins - drug effects Actins - physiology Animals Antineoplastic Agents - pharmacology Cell Differentiation - drug effects Depsipeptides Genes, Reporter Green Fluorescent Proteins Luminescent Proteins - metabolism Movement Nerve Growth Factor - pharmacology Neuropeptides - metabolism Original Oxazoles - pharmacology PC12 Cells Peptides, Cyclic - pharmacology Pheochromocytoma Rats Recombinant Proteins - metabolism Secretory Vesicles - drug effects Secretory Vesicles - physiology Secretory Vesicles - ultrastructure |
| title | Physical mobilization of secretory vesicles facilitates neuropeptide release by nerve growth factor-differentiated PC12 Cells |
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