Automated high-throughput purification of 6xHis-tagged proteins

Methods based on immobilized-metal affinity chromatography (IMAC) technology for the isolation of 6xHis-tagged proteins offer a one-step purification process that is both robust and meets the challenge of quantitatively purifying thousands of proteins with differing structures and characteristics. T...

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Veröffentlicht in:Journal of biomolecular techniques 2002-09, Vol.13 (3), p.131-142
Hauptverfasser: Schafer, Frank, Römer, Ulla, Emmerlich, Melanie, Blümer, Julia, Lubenow, Helge, Steinert, Kerstin
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Sprache:eng
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