Relationship of Ca2+ sparks to STOCs studied with 2D and 3D imaging in feline oesophageal smooth muscle cells
We recorded Ca 2+ sparks and spontaneous transient outward currents (STOCs) simultaneously in smooth muscle cells using whole-cell patch recording and a unique, high-speed widefield digital imaging system to monitor fluo-3 fluorescence in both two and three dimensions (2D and 3D). In 2D imaging, the...
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| Veröffentlicht in: | The Journal of physiology 2001-03, Vol.531 (2), p.315-327 |
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| container_end_page | 327 |
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| container_title | The Journal of physiology |
| container_volume | 531 |
| creator | Michael T Kirber Elaine F Etter Karl A Bellvà Lawrence M Lifshitz Richard A Tuft Fredric S Fay John V Walsh, Jr Kevin E Fogarty |
| description | We recorded Ca 2+ sparks and spontaneous transient outward currents (STOCs) simultaneously in smooth muscle cells using whole-cell patch recording
and a unique, high-speed widefield digital imaging system to monitor fluo-3 fluorescence in both two and three dimensions
(2D and 3D).
In 2D imaging, the correlation between the amplitude of a spark and its corresponding STOC was a weak one, and 27 % of the
sparks failed to cause STOCs. The STOCless sparks were not significantly different in amplitude from those that caused STOCs.
Three-dimensional imaging disclosed that STOCless sparks were located close to the cell surface, and on average their apparent
distance from the cell surface was not significantly different from the sparks that cause STOCs.
Statistical evaluation of spark clusters disclosed that there were regions of the cell where the probability of spark occurrence
was high and others where it was quite low. |
| doi_str_mv | 10.1111/j.1469-7793.2001.0315i.x |
| format | Article |
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and a unique, high-speed widefield digital imaging system to monitor fluo-3 fluorescence in both two and three dimensions
(2D and 3D).
In 2D imaging, the correlation between the amplitude of a spark and its corresponding STOC was a weak one, and 27 % of the
sparks failed to cause STOCs. The STOCless sparks were not significantly different in amplitude from those that caused STOCs.
Three-dimensional imaging disclosed that STOCless sparks were located close to the cell surface, and on average their apparent
distance from the cell surface was not significantly different from the sparks that cause STOCs.
Statistical evaluation of spark clusters disclosed that there were regions of the cell where the probability of spark occurrence
was high and others where it was quite low.</description><identifier>ISSN: 0022-3751</identifier><identifier>EISSN: 1469-7793</identifier><identifier>DOI: 10.1111/j.1469-7793.2001.0315i.x</identifier><identifier>PMID: 11230506</identifier><language>eng</language><publisher>Oxford, UK: The Physiological Society</publisher><subject>Aniline Compounds ; Animals ; Calcium - physiology ; Cats ; Cell Membrane - physiology ; Electric Conductivity ; Esophagus - cytology ; Esophagus - physiology ; Fluorescent Dyes ; Image Processing, Computer-Assisted ; Imaging, Three-Dimensional ; Muscle, Smooth - cytology ; Muscle, Smooth - physiology ; Original ; Patch-Clamp Techniques ; Xanthenes</subject><ispartof>The Journal of physiology, 2001-03, Vol.531 (2), p.315-327</ispartof><rights>2001 The Journal of Physiology © 2001 The Physiological Society</rights><rights>The Physiological Society 2001 2001</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2278474/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2278474/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,1411,1427,27901,27902,45550,45551,46384,46808,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11230506$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Michael T Kirber</creatorcontrib><creatorcontrib>Elaine F Etter</creatorcontrib><creatorcontrib>Karl A BellvÃ</creatorcontrib><creatorcontrib>Lawrence M Lifshitz</creatorcontrib><creatorcontrib>Richard A Tuft</creatorcontrib><creatorcontrib>Fredric S Fay</creatorcontrib><creatorcontrib>John V Walsh, Jr</creatorcontrib><creatorcontrib>Kevin E Fogarty</creatorcontrib><title>Relationship of Ca2+ sparks to STOCs studied with 2D and 3D imaging in feline oesophageal smooth muscle cells</title><title>The Journal of physiology</title><addtitle>J Physiol</addtitle><description>We recorded Ca 2+ sparks and spontaneous transient outward currents (STOCs) simultaneously in smooth muscle cells using whole-cell patch recording
and a unique, high-speed widefield digital imaging system to monitor fluo-3 fluorescence in both two and three dimensions
(2D and 3D).
In 2D imaging, the correlation between the amplitude of a spark and its corresponding STOC was a weak one, and 27 % of the
sparks failed to cause STOCs. The STOCless sparks were not significantly different in amplitude from those that caused STOCs.
Three-dimensional imaging disclosed that STOCless sparks were located close to the cell surface, and on average their apparent
distance from the cell surface was not significantly different from the sparks that cause STOCs.
Statistical evaluation of spark clusters disclosed that there were regions of the cell where the probability of spark occurrence
was high and others where it was quite low.</description><subject>Aniline Compounds</subject><subject>Animals</subject><subject>Calcium - physiology</subject><subject>Cats</subject><subject>Cell Membrane - physiology</subject><subject>Electric Conductivity</subject><subject>Esophagus - cytology</subject><subject>Esophagus - physiology</subject><subject>Fluorescent Dyes</subject><subject>Image Processing, Computer-Assisted</subject><subject>Imaging, Three-Dimensional</subject><subject>Muscle, Smooth - cytology</subject><subject>Muscle, Smooth - physiology</subject><subject>Original</subject><subject>Patch-Clamp Techniques</subject><subject>Xanthenes</subject><issn>0022-3751</issn><issn>1469-7793</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkVtv1DAQhS1ERZfCX0B-ggeU1JfEiSWEhLblpkpFsDyP7GQ28ZLEaZx0u_-epFsKWLI80vnmjO1DCOUs5vM638U8UTrKMi1jwRiPmeSpi--ekNWj8JSsGBMiklnKT8nzEHYzKJnWz8gp50KylKkVab9jY0bnu1C7nvotXRvxlobeDL8CHT39sbleBxrGqXRY0r0bayouqOlKKi-oa03luoq6jm6xcR1Sj8H3tanQNDS03s94O4WiQVpg04QX5GRrmoAvH84z8vPj5Wb9Obq6_vRl_eEqqqXQaWQQC1aaBDWaRGVaCWtQCbTzZqXNU6G1Tq2ypc3QIpdapYWVMsdcZYlR8oy8P_r2k22xLLAbB9NAP8w3Hg7gjYP_lc7VUPlbECLLkyyZDV4_GAz-ZsIwQuvC8gTToZ8CZErnjN-Dr_6d9Djizw_PwLsjsHcNHv7qDJYkYQdLYLAEBkuScJ8k3MHm67e5mtvfHNtrV9V7NyD09SE4H3zhcDxAKjkIWMjfznihDw</recordid><startdate>20010301</startdate><enddate>20010301</enddate><creator>Michael T Kirber</creator><creator>Elaine F Etter</creator><creator>Karl A BellvÃ</creator><creator>Lawrence M Lifshitz</creator><creator>Richard A Tuft</creator><creator>Fredric S Fay</creator><creator>John V Walsh, Jr</creator><creator>Kevin E Fogarty</creator><general>The Physiological Society</general><general>Blackwell Science Ltd</general><general>Blackwell Science Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20010301</creationdate><title>Relationship of Ca2+ sparks to STOCs studied with 2D and 3D imaging in feline oesophageal smooth muscle cells</title><author>Michael T Kirber ; Elaine F Etter ; Karl A Bellvà ; Lawrence M Lifshitz ; Richard A Tuft ; Fredric S Fay ; John V Walsh, Jr ; Kevin E Fogarty</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h3295-aeec0da4e9ea467962bae62eb62e0db8529995b6bdb7ebe13965cb338e8674a63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Aniline Compounds</topic><topic>Animals</topic><topic>Calcium - physiology</topic><topic>Cats</topic><topic>Cell Membrane - physiology</topic><topic>Electric Conductivity</topic><topic>Esophagus - cytology</topic><topic>Esophagus - physiology</topic><topic>Fluorescent Dyes</topic><topic>Image Processing, Computer-Assisted</topic><topic>Imaging, Three-Dimensional</topic><topic>Muscle, Smooth - cytology</topic><topic>Muscle, Smooth - physiology</topic><topic>Original</topic><topic>Patch-Clamp Techniques</topic><topic>Xanthenes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Michael T Kirber</creatorcontrib><creatorcontrib>Elaine F Etter</creatorcontrib><creatorcontrib>Karl A BellvÃ</creatorcontrib><creatorcontrib>Lawrence M Lifshitz</creatorcontrib><creatorcontrib>Richard A Tuft</creatorcontrib><creatorcontrib>Fredric S Fay</creatorcontrib><creatorcontrib>John V Walsh, Jr</creatorcontrib><creatorcontrib>Kevin E Fogarty</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Michael T Kirber</au><au>Elaine F Etter</au><au>Karl A BellvÃ</au><au>Lawrence M Lifshitz</au><au>Richard A Tuft</au><au>Fredric S Fay</au><au>John V Walsh, Jr</au><au>Kevin E Fogarty</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Relationship of Ca2+ sparks to STOCs studied with 2D and 3D imaging in feline oesophageal smooth muscle cells</atitle><jtitle>The Journal of physiology</jtitle><addtitle>J Physiol</addtitle><date>2001-03-01</date><risdate>2001</risdate><volume>531</volume><issue>2</issue><spage>315</spage><epage>327</epage><pages>315-327</pages><issn>0022-3751</issn><eissn>1469-7793</eissn><abstract>We recorded Ca 2+ sparks and spontaneous transient outward currents (STOCs) simultaneously in smooth muscle cells using whole-cell patch recording
and a unique, high-speed widefield digital imaging system to monitor fluo-3 fluorescence in both two and three dimensions
(2D and 3D).
In 2D imaging, the correlation between the amplitude of a spark and its corresponding STOC was a weak one, and 27 % of the
sparks failed to cause STOCs. The STOCless sparks were not significantly different in amplitude from those that caused STOCs.
Three-dimensional imaging disclosed that STOCless sparks were located close to the cell surface, and on average their apparent
distance from the cell surface was not significantly different from the sparks that cause STOCs.
Statistical evaluation of spark clusters disclosed that there were regions of the cell where the probability of spark occurrence
was high and others where it was quite low.</abstract><cop>Oxford, UK</cop><pub>The Physiological Society</pub><pmid>11230506</pmid><doi>10.1111/j.1469-7793.2001.0315i.x</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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| source | Wiley Free Content; MEDLINE; IngentaConnect Free/Open Access Journals; Wiley Online Library Journals Frontfile Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Aniline Compounds Animals Calcium - physiology Cats Cell Membrane - physiology Electric Conductivity Esophagus - cytology Esophagus - physiology Fluorescent Dyes Image Processing, Computer-Assisted Imaging, Three-Dimensional Muscle, Smooth - cytology Muscle, Smooth - physiology Original Patch-Clamp Techniques Xanthenes |
| title | Relationship of Ca2+ sparks to STOCs studied with 2D and 3D imaging in feline oesophageal smooth muscle cells |
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