Resistin does not down-regulate the transcription of insulin receptor promoter
Objective： To detect the effect of resistin on the transcription of insulin receptor promoter. Methods： Luciferase reporter gene was fused downstream of human insulin receptor promoter and the enzymatic activity of luciferase was determined in the presence or absence of resistin. The resistin expres...
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| Veröffentlicht in: | Journal of Zhejiang University. B. Science 2008-04, Vol.9 (4), p.313-318 |
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| creator | Qiao, Xiao-zhi Wang, Xian-feng Xu, Zhe-rong Yang, Yun-mei |
| description | Objective： To detect the effect of resistin on the transcription of insulin receptor promoter. Methods： Luciferase reporter gene was fused downstream of human insulin receptor promoter and the enzymatic activity of luciferase was determined in the presence or absence of resistin. The resistin expressed with plasmid was stained with antibody against Myc tag which was in frame fused with resistin coding sequence, and then imaged with confocal microscopy. Results： The treatment of pIRP-LUC transfected cells with recombinant resistin did not result in significant difference in the enzymatic activity of luciferase compared to the untreated cells. Cell staining showed that green fluorescence could be observed in the cytoplasm, but not in the nucleus. Conclusion： The results suggest that the endogenous resistin may functionally locate in the cytoplasm, but does not enter the nucleus and not down-regulate the transcription of insulin receptor promoter. |
| doi_str_mv | 10.1631/jzus.B0710637 |
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Methods： Luciferase reporter gene was fused downstream of human insulin receptor promoter and the enzymatic activity of luciferase was determined in the presence or absence of resistin. The resistin expressed with plasmid was stained with antibody against Myc tag which was in frame fused with resistin coding sequence, and then imaged with confocal microscopy. Results： The treatment of pIRP-LUC transfected cells with recombinant resistin did not result in significant difference in the enzymatic activity of luciferase compared to the untreated cells. Cell staining showed that green fluorescence could be observed in the cytoplasm, but not in the nucleus. Conclusion： The results suggest that the endogenous resistin may functionally locate in the cytoplasm, but does not enter the nucleus and not down-regulate the transcription of insulin receptor promoter.</description><identifier>ISSN: 1673-1581</identifier><identifier>EISSN: 1862-1783</identifier><identifier>DOI: 10.1631/jzus.B0710637</identifier><identifier>PMID: 18381806</identifier><language>eng</language><publisher>Heidelberg: SP Zhejiang University Press</publisher><subject>Biomedical and Life Sciences ; Biomedicine ; Cell Line ; Cell Nucleus - metabolism ; Cytoplasm - metabolism ; Down-Regulation ; Gene Expression Regulation ; Humans ; Insulin ; Luciferases - metabolism ; Microscopy, Confocal ; Models, Biological ; Plasmids - metabolism ; Promoter Regions, Genetic ; Proteins ; Receptor, Insulin - biosynthesis ; Receptor, Insulin - genetics ; Resistin - pharmacology ; Transcription, Genetic ; Transfection ; 启动子 ; 胰岛素 ; 荧光素酶 ; 调整因子</subject><ispartof>Journal of Zhejiang University. B. Science, 2008-04, Vol.9 (4), p.313-318</ispartof><rights>Zhejiang University Press 2008</rights><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><rights>Copyright © 2008, Journal of Zhejiang University Science 2008</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c544t-970062a29d0e049341847bedd2a723fe7b48bfb4693adcea2732dbbba6f5ea943</citedby><cites>FETCH-LOGICAL-c544t-970062a29d0e049341847bedd2a723fe7b48bfb4693adcea2732dbbba6f5ea943</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/86281A/86281A.jpg</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2276674/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2276674/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,315,728,781,785,886,27926,27927,41490,42559,51321,53793,53795</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18381806$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Qiao, Xiao-zhi</creatorcontrib><creatorcontrib>Wang, Xian-feng</creatorcontrib><creatorcontrib>Xu, Zhe-rong</creatorcontrib><creatorcontrib>Yang, Yun-mei</creatorcontrib><title>Resistin does not down-regulate the transcription of insulin receptor promoter</title><title>Journal of Zhejiang University. B. Science</title><addtitle>J. Zhejiang Univ. Sci. B</addtitle><addtitle>Journal of Zhejiang University Science</addtitle><description>Objective： To detect the effect of resistin on the transcription of insulin receptor promoter. Methods： Luciferase reporter gene was fused downstream of human insulin receptor promoter and the enzymatic activity of luciferase was determined in the presence or absence of resistin. The resistin expressed with plasmid was stained with antibody against Myc tag which was in frame fused with resistin coding sequence, and then imaged with confocal microscopy. Results： The treatment of pIRP-LUC transfected cells with recombinant resistin did not result in significant difference in the enzymatic activity of luciferase compared to the untreated cells. Cell staining showed that green fluorescence could be observed in the cytoplasm, but not in the nucleus. Conclusion： The results suggest that the endogenous resistin may functionally locate in the cytoplasm, but does not enter the nucleus and not down-regulate the transcription of insulin receptor promoter.</description><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cell Line</subject><subject>Cell Nucleus - metabolism</subject><subject>Cytoplasm - metabolism</subject><subject>Down-Regulation</subject><subject>Gene Expression Regulation</subject><subject>Humans</subject><subject>Insulin</subject><subject>Luciferases - metabolism</subject><subject>Microscopy, Confocal</subject><subject>Models, Biological</subject><subject>Plasmids - metabolism</subject><subject>Promoter Regions, Genetic</subject><subject>Proteins</subject><subject>Receptor, Insulin - biosynthesis</subject><subject>Receptor, Insulin - genetics</subject><subject>Resistin - pharmacology</subject><subject>Transcription, Genetic</subject><subject>Transfection</subject><subject>启动子</subject><subject>胰岛素</subject><subject>荧光素酶</subject><subject>调整因子</subject><issn>1673-1581</issn><issn>1862-1783</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkdtrFDEUxoNY7EUffZXBBx8Ks81tk8yLoMUblAqizyGZOTObdTaZJhlb-9ebZde2CuJDyIHzO9_5kg-h5wQviGDkbH07p8VbLAkWTD5CR0QJWhOp2ONSC8lqslTkEB2ntMaYcyzFE3RIFFNEYXGELr9Acik7X3UBUuVDLsW1ryMM82gyVHlVTjQ-tdFN2QVfhb5yPs1jmYnQwpRDrKYYNiFDfIoOejMmeLa_T9C39---nn-sLz5_-HT-5qJul5znupEYC2po02HAvGGcKC4tdB01krIepOXK9paLhpmuBUMlo5211oh-Cabh7AS93ulOs91AQXzxOOopuo2JP3UwTv_Z8W6lh_BDUyqFkFuB053AtfG98YNehzn6YlnfrrubG2s1UIwV5sVogV_tt8VwNUPKeuNSC-NoPIQ5aYm5UESy_4KMlsgI3oIv_wLv9ivFmKKiaQpU76A2hpQi9HfvI1hvw9fb8PXv8Av_4uGn3NP7tAuw2AGptPwA8X7rvxT3NttV8MNVmdHWtN97N4IuFjETzZL9Ao0ax54</recordid><startdate>20080401</startdate><enddate>20080401</enddate><creator>Qiao, 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genetics</topic><topic>Resistin - pharmacology</topic><topic>Transcription, Genetic</topic><topic>Transfection</topic><topic>启动子</topic><topic>胰岛素</topic><topic>荧光素酶</topic><topic>调整因子</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Qiao, Xiao-zhi</creatorcontrib><creatorcontrib>Wang, Xian-feng</creatorcontrib><creatorcontrib>Xu, Zhe-rong</creatorcontrib><creatorcontrib>Yang, Yun-mei</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Biotechnology Research 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B. Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Qiao, Xiao-zhi</au><au>Wang, Xian-feng</au><au>Xu, Zhe-rong</au><au>Yang, Yun-mei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Resistin does not down-regulate the transcription of insulin receptor promoter</atitle><jtitle>Journal of Zhejiang University. B. Science</jtitle><stitle>J. Zhejiang Univ. Sci. B</stitle><addtitle>Journal of Zhejiang University Science</addtitle><date>2008-04-01</date><risdate>2008</risdate><volume>9</volume><issue>4</issue><spage>313</spage><epage>318</epage><pages>313-318</pages><issn>1673-1581</issn><eissn>1862-1783</eissn><abstract>Objective： To detect the effect of resistin on the transcription of insulin receptor promoter. Methods： Luciferase reporter gene was fused downstream of human insulin receptor promoter and the enzymatic activity of luciferase was determined in the presence or absence of resistin. The resistin expressed with plasmid was stained with antibody against Myc tag which was in frame fused with resistin coding sequence, and then imaged with confocal microscopy. Results： The treatment of pIRP-LUC transfected cells with recombinant resistin did not result in significant difference in the enzymatic activity of luciferase compared to the untreated cells. Cell staining showed that green fluorescence could be observed in the cytoplasm, but not in the nucleus. Conclusion： The results suggest that the endogenous resistin may functionally locate in the cytoplasm, but does not enter the nucleus and not down-regulate the transcription of insulin receptor promoter.</abstract><cop>Heidelberg</cop><pub>SP Zhejiang University Press</pub><pmid>18381806</pmid><doi>10.1631/jzus.B0710637</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Biomedical and Life Sciences Biomedicine Cell Line Cell Nucleus - metabolism Cytoplasm - metabolism Down-Regulation Gene Expression Regulation Humans Insulin Luciferases - metabolism Microscopy, Confocal Models, Biological Plasmids - metabolism Promoter Regions, Genetic Proteins Receptor, Insulin - biosynthesis Receptor, Insulin - genetics Resistin - pharmacology Transcription, Genetic Transfection 启动子 胰岛素 荧光素酶 调整因子 |
| title | Resistin does not down-regulate the transcription of insulin receptor promoter |
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