Baculovirus-mediated expression, purification, and characterization of a fully activated catalytic kinase domain construct of the 70 kDa 40S ribosomal protein S6 kinase-1 αII isoform (S6K1αII)

S6K1αII is a member of the AGC subfamily of serine–threonine protein kinases, whereby catalytic activation requires dual phosphorylation of critical residues in the conserved T-loop (T229) and hydrophobic motif (HM; T389) regions of its catalytic kinase domain [S6K1αII(ΔAID); deletion of C-terminal...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Protein expression and purification 2008-03, Vol.58 (1), p.32-41
Hauptverfasser:	Keshwani, Malik M., Ross, Duncan B., Ragan, Timothy J., Harris, Thomas K.
Format:	Artikel
Sprache:	eng
Schlagworte:	Baculoviral coinfection Baculovirus ESI-TOF MonoQ anion exchange PDK1 Phosphoinositide-dependent protein kinase-1 Protein phosphorylation S6K1-T389E Sf9 insect cells
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!