An Important Role for the Multienzyme Aminoacyl-tRNA Synthetase Complex in Mammalian Translation and Cell Growth
In mammalian cells, aminoacyl-tRNA synthetases (aaRSs) are organized into a high-molecular-weight multisynthetase complex whose cellular function has remained a mystery. In this study, we have taken advantage of the fact that mammalian cells contain two forms of ArgRS, both products of the same gene...
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| Veröffentlicht in: | Molecular cell 2008-02, Vol.29 (4), p.419-427 |
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| creator | Kyriacou, Sophia V. Deutscher, Murray P. |
| description | In mammalian cells, aminoacyl-tRNA synthetases (aaRSs) are organized into a high-molecular-weight multisynthetase complex whose cellular function has remained a mystery. In this study, we have taken advantage of the fact that mammalian cells contain two forms of ArgRS, both products of the same gene, to investigate the complex's physiological role. The data indicate that the high-molecular-weight form of ArgRS, which is present exclusively as an integral component of the multisynthetase complex, is essential for normal protein synthesis and growth of CHO cells even when low-molecular-weight, free ArgRS is present and Arg-tRNA continues to be synthesized at close to wild-type levels. Based on these observations, we conclude that Arg-tRNA generated by the synthetase complex is a more efficient precursor for protein synthesis than Arg-tRNA generated by free ArgRS, exactly as would be predicted by the channeling model for mammalian translation. |
| doi_str_mv | 10.1016/j.molcel.2007.11.038 |
| format | Article |
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| source | MEDLINE; Cell Press Free Archives; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Free Full-Text Journals in Chemistry |
| subjects | Amino Acyl-tRNA Synthetases - genetics Amino Acyl-tRNA Synthetases - metabolism Animals Cell Proliferation CHO Cells Codon, Initiator Cricetinae Cricetulus Isoenzymes - genetics Isoenzymes - metabolism Multienzyme Complexes - chemistry Multienzyme Complexes - metabolism Point Mutation Protein Biosynthesis PROTEINS RNA RNA, Transfer, Amino Acid-Specific - genetics RNA, Transfer, Amino Acid-Specific - metabolism Transfer RNA Aminoacylation |
| title | An Important Role for the Multienzyme Aminoacyl-tRNA Synthetase Complex in Mammalian Translation and Cell Growth |
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