Incidence and nature of peritoneal catheter biofilm determined by electron and confocal laser scanning microscopy
Thirty-two Tenckhoff catheters retrieved from continuous ambulatory peritoneal dialysis patients with a history of peritonitis were examined for microbial biofilm. Confocal laser scanning microscopy was successfully employed to visualize bacteria in biofilm occluded from view by scanning electron mi...
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| Veröffentlicht in: | Epidemiology and infection 1994-06, Vol.112 (3), p.551-559 |
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| creator | Gorman, S. P. Adair, C. G. Mawhinney, W. M. |
| description | Thirty-two Tenckhoff catheters retrieved from continuous ambulatory peritoneal dialysis patients with a history of peritonitis were examined for microbial biofilm. Confocal laser scanning microscopy was successfully employed to visualize bacteria in biofilm occluded from view by scanning electron microscopy. Occluded but viable microbial biofilm was associated with 17 (81%) catheters from patients free from infection following renal transplant. Mixed isolate biofilm with two or more isolates of coagulase-negative staphylococci or Staphylococcus aureus was found on 41% of these catheters. Clearly visible viable biofilm consisting exclusively of Pseudomonas aeruginosa occurred on all four catheters removed due to recurrent peritonitis. Five (71%) catheters retrieved from patients transferred to haemodialysis had viable biofilm. Antibiotic sensitivities of the biofilm isolates were similar in profile to those reported for non-biofilm isolates from infected dialysate. Persistence of catheter biofilm despite direct contact with therapeutic levels of antibiotics in peritoneal dialysate requires that attention be directed towards improving antibiotic efficacy against peritonitis-causing bacteria in biofilm form. |
| doi_str_mv | 10.1017/S0950268800051256 |
| format | Article |
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P. ; Adair, C. G. ; Mawhinney, W. M.</creator><creatorcontrib>Gorman, S. P. ; Adair, C. G. ; Mawhinney, W. M.</creatorcontrib><description>Thirty-two Tenckhoff catheters retrieved from continuous ambulatory peritoneal dialysis patients with a history of peritonitis were examined for microbial biofilm. Confocal laser scanning microscopy was successfully employed to visualize bacteria in biofilm occluded from view by scanning electron microscopy. Occluded but viable microbial biofilm was associated with 17 (81%) catheters from patients free from infection following renal transplant. Mixed isolate biofilm with two or more isolates of coagulase-negative staphylococci or Staphylococcus aureus was found on 41% of these catheters. Clearly visible viable biofilm consisting exclusively of Pseudomonas aeruginosa occurred on all four catheters removed due to recurrent peritonitis. Five (71%) catheters retrieved from patients transferred to haemodialysis had viable biofilm. Antibiotic sensitivities of the biofilm isolates were similar in profile to those reported for non-biofilm isolates from infected dialysate. Persistence of catheter biofilm despite direct contact with therapeutic levels of antibiotics in peritoneal dialysate requires that attention be directed towards improving antibiotic efficacy against peritonitis-causing bacteria in biofilm form.</description><identifier>ISSN: 0950-2688</identifier><identifier>EISSN: 1469-4409</identifier><identifier>DOI: 10.1017/S0950268800051256</identifier><identifier>PMID: 8005221</identifier><identifier>CODEN: EPINEU</identifier><language>eng</language><publisher>Cambridge, UK: Cambridge University Press</publisher><subject>Antibiotics ; Bacteria ; Bacterial Adhesion ; Bacteriological methods and techniques used in bacteriology ; Bacteriology ; Biofilms ; Biological and medical sciences ; Catheters ; Catheters, Indwelling - adverse effects ; Continuous ambulatory peritoneal dialysis ; Equipment Contamination ; Fundamental and applied biological sciences. Psychology ; Humans ; Incidence ; Lasers ; Microbial Sensitivity Tests ; Microbiology ; Microorganisms ; Microscopy ; Microscopy, Electron ; Microscopy, Electron, Scanning ; Microscopy, Fluorescence ; Peritoneal Dialysis, Continuous Ambulatory ; Peritonitis ; Peritonitis - etiology ; Peritonitis - microbiology ; Pseudomonas aeruginosa - drug effects ; Pseudomonas aeruginosa - isolation & purification ; Pseudomonas aeruginosa - ultrastructure ; Recurrence ; Staphylococcus ; Staphylococcus - drug effects ; Staphylococcus - isolation & purification ; Staphylococcus - ultrastructure</subject><ispartof>Epidemiology and infection, 1994-06, Vol.112 (3), p.551-559</ispartof><rights>Copyright © Cambridge University Press 1994</rights><rights>Copyright 1994 Cambridge University Press</rights><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c557t-7115a2ebaf00ed8fef4f65cc8047c5a491db959600e896dfc514b9347cf84fb63</citedby><cites>FETCH-LOGICAL-c557t-7115a2ebaf00ed8fef4f65cc8047c5a491db959600e896dfc514b9347cf84fb63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/3864361$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/3864361$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27924,27925,53791,53793,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4104085$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8005221$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gorman, S. P.</creatorcontrib><creatorcontrib>Adair, C. G.</creatorcontrib><creatorcontrib>Mawhinney, W. M.</creatorcontrib><title>Incidence and nature of peritoneal catheter biofilm determined by electron and confocal laser scanning microscopy</title><title>Epidemiology and infection</title><addtitle>Epidemiol. Infect</addtitle><description>Thirty-two Tenckhoff catheters retrieved from continuous ambulatory peritoneal dialysis patients with a history of peritonitis were examined for microbial biofilm. Confocal laser scanning microscopy was successfully employed to visualize bacteria in biofilm occluded from view by scanning electron microscopy. Occluded but viable microbial biofilm was associated with 17 (81%) catheters from patients free from infection following renal transplant. Mixed isolate biofilm with two or more isolates of coagulase-negative staphylococci or Staphylococcus aureus was found on 41% of these catheters. Clearly visible viable biofilm consisting exclusively of Pseudomonas aeruginosa occurred on all four catheters removed due to recurrent peritonitis. Five (71%) catheters retrieved from patients transferred to haemodialysis had viable biofilm. Antibiotic sensitivities of the biofilm isolates were similar in profile to those reported for non-biofilm isolates from infected dialysate. Persistence of catheter biofilm despite direct contact with therapeutic levels of antibiotics in peritoneal dialysate requires that attention be directed towards improving antibiotic efficacy against peritonitis-causing bacteria in biofilm form.</description><subject>Antibiotics</subject><subject>Bacteria</subject><subject>Bacterial Adhesion</subject><subject>Bacteriological methods and techniques used in bacteriology</subject><subject>Bacteriology</subject><subject>Biofilms</subject><subject>Biological and medical sciences</subject><subject>Catheters</subject><subject>Catheters, Indwelling - adverse effects</subject><subject>Continuous ambulatory peritoneal dialysis</subject><subject>Equipment Contamination</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Incidence</subject><subject>Lasers</subject><subject>Microbial Sensitivity Tests</subject><subject>Microbiology</subject><subject>Microorganisms</subject><subject>Microscopy</subject><subject>Microscopy, Electron</subject><subject>Microscopy, Electron, Scanning</subject><subject>Microscopy, Fluorescence</subject><subject>Peritoneal Dialysis, Continuous Ambulatory</subject><subject>Peritonitis</subject><subject>Peritonitis - etiology</subject><subject>Peritonitis - microbiology</subject><subject>Pseudomonas aeruginosa - drug effects</subject><subject>Pseudomonas aeruginosa - isolation & purification</subject><subject>Pseudomonas aeruginosa - ultrastructure</subject><subject>Recurrence</subject><subject>Staphylococcus</subject><subject>Staphylococcus - drug effects</subject><subject>Staphylococcus - isolation & purification</subject><subject>Staphylococcus - ultrastructure</subject><issn>0950-2688</issn><issn>1469-4409</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV-L1DAUxYso6-zqBxAU-iD7Vk3a_H0RllXXhQGVVRBfQprezGZsk9mkFefbmzplUAR9CpffOYfce4riCUYvMML85Q2SFNVMCIQQxTVl94oVJkxWhCB5v1jNuJr5w-I0pW1WyVrwk-IkG2hd41Vxd-2N68AbKLXvSq_HKUIZbLmD6MbgQfel0eMtjBDL1gXr-qHs5mlwHrqy3ZfQgxlj8L8CTPA2mGzqdcqOZLT3zm_KwZkYkgm7_aPigdV9gsfLe1Z8fvvm0-W7av3-6vryYl0ZSvlYcYyprqHVFiHohAVLLKPGCES4oZpI3LWSSpapkKyzhmLSyiZDK4htWXNWvDrk7qZ2gM6AH6Pu1S66Qce9CtqpP4l3t2oTvqu65pgingPOl4AY7iZIoxpcMtD32kOYkuKMEloT8V8hplLWkuMsxAfhfIsUwR5_g5GaC1V_FZo9z35f4-hYGsz8-cJ1PnZvo86FpqOMYESQoFn29CDbpjHEI24EIw2bU6oDdmmEH0es4zfFeMOpYlcfFfnAxdfXX9bqJuubZRM9tNF1G1DbMEWfC_3HLj8BXGPS2w</recordid><startdate>19940601</startdate><enddate>19940601</enddate><creator>Gorman, S. P.</creator><creator>Adair, C. G.</creator><creator>Mawhinney, W. M.</creator><general>Cambridge University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19940601</creationdate><title>Incidence and nature of peritoneal catheter biofilm determined by electron and confocal laser scanning microscopy</title><author>Gorman, S. P. ; Adair, C. G. ; Mawhinney, W. M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c557t-7115a2ebaf00ed8fef4f65cc8047c5a491db959600e896dfc514b9347cf84fb63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Antibiotics</topic><topic>Bacteria</topic><topic>Bacterial Adhesion</topic><topic>Bacteriological methods and techniques used in bacteriology</topic><topic>Bacteriology</topic><topic>Biofilms</topic><topic>Biological and medical sciences</topic><topic>Catheters</topic><topic>Catheters, Indwelling - adverse effects</topic><topic>Continuous ambulatory peritoneal dialysis</topic><topic>Equipment Contamination</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Incidence</topic><topic>Lasers</topic><topic>Microbial Sensitivity Tests</topic><topic>Microbiology</topic><topic>Microorganisms</topic><topic>Microscopy</topic><topic>Microscopy, Electron</topic><topic>Microscopy, Electron, Scanning</topic><topic>Microscopy, Fluorescence</topic><topic>Peritoneal Dialysis, Continuous Ambulatory</topic><topic>Peritonitis</topic><topic>Peritonitis - etiology</topic><topic>Peritonitis - microbiology</topic><topic>Pseudomonas aeruginosa - drug effects</topic><topic>Pseudomonas aeruginosa - isolation & purification</topic><topic>Pseudomonas aeruginosa - ultrastructure</topic><topic>Recurrence</topic><topic>Staphylococcus</topic><topic>Staphylococcus - drug effects</topic><topic>Staphylococcus - isolation & purification</topic><topic>Staphylococcus - ultrastructure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gorman, S. P.</creatorcontrib><creatorcontrib>Adair, C. G.</creatorcontrib><creatorcontrib>Mawhinney, W. M.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Epidemiology and infection</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gorman, S. P.</au><au>Adair, C. G.</au><au>Mawhinney, W. M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Incidence and nature of peritoneal catheter biofilm determined by electron and confocal laser scanning microscopy</atitle><jtitle>Epidemiology and infection</jtitle><addtitle>Epidemiol. Infect</addtitle><date>1994-06-01</date><risdate>1994</risdate><volume>112</volume><issue>3</issue><spage>551</spage><epage>559</epage><pages>551-559</pages><issn>0950-2688</issn><eissn>1469-4409</eissn><coden>EPINEU</coden><abstract>Thirty-two Tenckhoff catheters retrieved from continuous ambulatory peritoneal dialysis patients with a history of peritonitis were examined for microbial biofilm. Confocal laser scanning microscopy was successfully employed to visualize bacteria in biofilm occluded from view by scanning electron microscopy. Occluded but viable microbial biofilm was associated with 17 (81%) catheters from patients free from infection following renal transplant. Mixed isolate biofilm with two or more isolates of coagulase-negative staphylococci or Staphylococcus aureus was found on 41% of these catheters. Clearly visible viable biofilm consisting exclusively of Pseudomonas aeruginosa occurred on all four catheters removed due to recurrent peritonitis. Five (71%) catheters retrieved from patients transferred to haemodialysis had viable biofilm. Antibiotic sensitivities of the biofilm isolates were similar in profile to those reported for non-biofilm isolates from infected dialysate. Persistence of catheter biofilm despite direct contact with therapeutic levels of antibiotics in peritoneal dialysate requires that attention be directed towards improving antibiotic efficacy against peritonitis-causing bacteria in biofilm form.</abstract><cop>Cambridge, UK</cop><pub>Cambridge University Press</pub><pmid>8005221</pmid><doi>10.1017/S0950268800051256</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Epidemiology and infection, 1994-06, Vol.112 (3), p.551-559 |
| issn | 0950-2688 1469-4409 |
| language | eng |
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| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central |
| subjects | Antibiotics Bacteria Bacterial Adhesion Bacteriological methods and techniques used in bacteriology Bacteriology Biofilms Biological and medical sciences Catheters Catheters, Indwelling - adverse effects Continuous ambulatory peritoneal dialysis Equipment Contamination Fundamental and applied biological sciences. Psychology Humans Incidence Lasers Microbial Sensitivity Tests Microbiology Microorganisms Microscopy Microscopy, Electron Microscopy, Electron, Scanning Microscopy, Fluorescence Peritoneal Dialysis, Continuous Ambulatory Peritonitis Peritonitis - etiology Peritonitis - microbiology Pseudomonas aeruginosa - drug effects Pseudomonas aeruginosa - isolation & purification Pseudomonas aeruginosa - ultrastructure Recurrence Staphylococcus Staphylococcus - drug effects Staphylococcus - isolation & purification Staphylococcus - ultrastructure |
| title | Incidence and nature of peritoneal catheter biofilm determined by electron and confocal laser scanning microscopy |
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