Temporal effects of freezing on plasma nitric oxide concentrations in ponies
The purpose of this study was to compare concentrations of nitric oxide (NO) in fresh plasma versus frozen plasma, and determine the temporal effects of freezing on jugular venous plasma NO concentrations in clinically healthy ponies. Twenty-eight helminth-naive ponies, aged from 4 to 6 mo, were rai...
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| Veröffentlicht in: | Canadian journal of veterinary research 2003-01, Vol.67 (1), p.72-74 |
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| creator | Hubert, Jeremy D Seahorn, Thomas L Klei, Thomas R Hosgood, Giselle Moore, Rustin M |
| description | The purpose of this study was to compare concentrations of nitric oxide (NO) in fresh plasma versus frozen plasma, and determine the temporal effects of freezing on jugular venous plasma NO concentrations in clinically healthy ponies. Twenty-eight helminth-naive ponies, aged from 4 to 6 mo, were raised and maintained under parasite-free conditions. Blood was collected from the jugular vein, centrifuged, and the plasma supernatant was analyzed fresh for NO concentrations using a chemiluminescent method. The remaining samples were aliquoted into 12 samples and stored at -70 degrees C until they were analyzed for NO concentration. Plasma NO concentration was measured at monthly intervals for 12 mo. There were significant differences in the plasma NO concentration across time compared with the baseline value at 1, 3, 4, 6, 7, and 11 mo. However, these values remained within the range for clinically healthy equids compared with concentrations of NO from horses with increased NO from diseased states. |
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Twenty-eight helminth-naive ponies, aged from 4 to 6 mo, were raised and maintained under parasite-free conditions. Blood was collected from the jugular vein, centrifuged, and the plasma supernatant was analyzed fresh for NO concentrations using a chemiluminescent method. The remaining samples were aliquoted into 12 samples and stored at -70 degrees C until they were analyzed for NO concentration. Plasma NO concentration was measured at monthly intervals for 12 mo. There were significant differences in the plasma NO concentration across time compared with the baseline value at 1, 3, 4, 6, 7, and 11 mo. However, these values remained within the range for clinically healthy equids compared with concentrations of NO from horses with increased NO from diseased states.</description><identifier>ISSN: 0830-9000</identifier><identifier>PMID: 12528834</identifier><language>eng</language><publisher>Canada</publisher><subject>Animals ; Blood Preservation - methods ; Blood Preservation - standards ; Blood Preservation - veterinary ; Female ; Freezing ; Horse Diseases - blood ; Horses - blood ; Male ; Nitric Oxide - blood ; Short Communication ; Specimen Handling - methods ; Specimen Handling - veterinary ; Time Factors</subject><ispartof>Canadian journal of veterinary research, 2003-01, Vol.67 (1), p.72-74</ispartof><rights>Copyright and/or publishing rights held by the Canadian Veterinary Medical Association</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC227032/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC227032/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12528834$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hubert, Jeremy D</creatorcontrib><creatorcontrib>Seahorn, Thomas L</creatorcontrib><creatorcontrib>Klei, Thomas R</creatorcontrib><creatorcontrib>Hosgood, Giselle</creatorcontrib><creatorcontrib>Moore, Rustin M</creatorcontrib><title>Temporal effects of freezing on plasma nitric oxide concentrations in ponies</title><title>Canadian journal of veterinary research</title><addtitle>Can J Vet Res</addtitle><description>The purpose of this study was to compare concentrations of nitric oxide (NO) in fresh plasma versus frozen plasma, and determine the temporal effects of freezing on jugular venous plasma NO concentrations in clinically healthy ponies. Twenty-eight helminth-naive ponies, aged from 4 to 6 mo, were raised and maintained under parasite-free conditions. Blood was collected from the jugular vein, centrifuged, and the plasma supernatant was analyzed fresh for NO concentrations using a chemiluminescent method. The remaining samples were aliquoted into 12 samples and stored at -70 degrees C until they were analyzed for NO concentration. Plasma NO concentration was measured at monthly intervals for 12 mo. There were significant differences in the plasma NO concentration across time compared with the baseline value at 1, 3, 4, 6, 7, and 11 mo. However, these values remained within the range for clinically healthy equids compared with concentrations of NO from horses with increased NO from diseased states.</description><subject>Animals</subject><subject>Blood Preservation - methods</subject><subject>Blood Preservation - standards</subject><subject>Blood Preservation - veterinary</subject><subject>Female</subject><subject>Freezing</subject><subject>Horse Diseases - blood</subject><subject>Horses - blood</subject><subject>Male</subject><subject>Nitric Oxide - blood</subject><subject>Short Communication</subject><subject>Specimen Handling - methods</subject><subject>Specimen Handling - veterinary</subject><subject>Time Factors</subject><issn>0830-9000</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkL1OAzEQhF2ASAi8AnJFd5K9vvh8BQWK-JMi0YTasp11MLqzD_uCgKcnKAFBtcV8M7OaIzJlSrCqZYxNyGkpL4xBI0GckAmHOSgl6ilZrrAfUjYdRe_RjYUmT31G_AxxQ1OkQ2dKb2gMYw6OpvewRupSdBjHbMaQYqFhR6UYsJyRY2-6gueHOyNPtzerxX21fLx7WFwvqwEkHyszB24lVygkM35ds9bORYtOGmHqhlsAL4U0HhQX1nJWW1CSNzvUoVMtiBm52ucOW9vjev9Lp4ccepM_dDJB_1dieNab9KYBGia-_ZcHf06vWyyj7kNx2HUmYtoW3UArVcvaHXjxt-i34Wc_8QXIJW1h</recordid><startdate>200301</startdate><enddate>200301</enddate><creator>Hubert, Jeremy D</creator><creator>Seahorn, Thomas L</creator><creator>Klei, Thomas R</creator><creator>Hosgood, Giselle</creator><creator>Moore, Rustin M</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>200301</creationdate><title>Temporal effects of freezing on plasma nitric oxide concentrations in ponies</title><author>Hubert, Jeremy D ; Seahorn, Thomas L ; Klei, Thomas R ; Hosgood, Giselle ; Moore, Rustin M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p261t-a521b618e360afd409b539ec6a3a471b22f636af2813bb104b28617afdcec8923</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Blood Preservation - methods</topic><topic>Blood Preservation - standards</topic><topic>Blood Preservation - veterinary</topic><topic>Female</topic><topic>Freezing</topic><topic>Horse Diseases - blood</topic><topic>Horses - blood</topic><topic>Male</topic><topic>Nitric Oxide - blood</topic><topic>Short Communication</topic><topic>Specimen Handling - methods</topic><topic>Specimen Handling - veterinary</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hubert, Jeremy D</creatorcontrib><creatorcontrib>Seahorn, Thomas L</creatorcontrib><creatorcontrib>Klei, Thomas R</creatorcontrib><creatorcontrib>Hosgood, Giselle</creatorcontrib><creatorcontrib>Moore, Rustin M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Canadian journal of veterinary research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hubert, Jeremy D</au><au>Seahorn, Thomas L</au><au>Klei, Thomas R</au><au>Hosgood, Giselle</au><au>Moore, Rustin M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Temporal effects of freezing on plasma nitric oxide concentrations in ponies</atitle><jtitle>Canadian journal of veterinary research</jtitle><addtitle>Can J Vet Res</addtitle><date>2003-01</date><risdate>2003</risdate><volume>67</volume><issue>1</issue><spage>72</spage><epage>74</epage><pages>72-74</pages><issn>0830-9000</issn><abstract>The purpose of this study was to compare concentrations of nitric oxide (NO) in fresh plasma versus frozen plasma, and determine the temporal effects of freezing on jugular venous plasma NO concentrations in clinically healthy ponies. 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| subjects | Animals Blood Preservation - methods Blood Preservation - standards Blood Preservation - veterinary Female Freezing Horse Diseases - blood Horses - blood Male Nitric Oxide - blood Short Communication Specimen Handling - methods Specimen Handling - veterinary Time Factors |
| title | Temporal effects of freezing on plasma nitric oxide concentrations in ponies |
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