Comparison of embryonated chicken eggs with MDCK cell culture for the isolation of swine influenza virus
Embryonated chicken eggs (ECE) and the Madin-Darby canine kidney (MDCK) cell line were compared for isolation of swine influenza virus (SIV) from nasal swabs and tissue samples. Samples originated from 30 pigs experimentally inoculated with 2 x 106 to 2 x 10(7) embryo infectious dose 50% (EID50)/mL...
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| Veröffentlicht in: | Canadian journal of veterinary research 2002-04, Vol.66 (2), p.117-121 |
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| creator | Clavijo, A Tresnan, D.B Jolie, R Zhou, E.M |
| description | Embryonated chicken eggs (ECE) and the Madin-Darby canine kidney (MDCK) cell line were compared for isolation of swine influenza virus (SIV) from nasal swabs and tissue samples. Samples originated from 30 pigs experimentally inoculated with 2 x 106 to 2 x 10(7) embryo infectious dose 50% (EID50)/mL of swine influenza strain A/Swine/Indiana/1726/88 (H1N1). The results were analyzed with McNemar's chi-squared test for symmetry. The results indicated that more samples were SIV-positive with ECE than with tissue culture (P < 0.001), suggesting that ECE remains the system of choice for isolation of SIV. It is recommend that routine use of both SIV isolation systems will increase the sensitivity of detection of virus shedding by considering the differences in growth and tropism of diverse SIV strains. |
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Samples originated from 30 pigs experimentally inoculated with 2 x 106 to 2 x 10(7) embryo infectious dose 50% (EID50)/mL of swine influenza strain A/Swine/Indiana/1726/88 (H1N1). The results were analyzed with McNemar's chi-squared test for symmetry. The results indicated that more samples were SIV-positive with ECE than with tissue culture (P < 0.001), suggesting that ECE remains the system of choice for isolation of SIV. It is recommend that routine use of both SIV isolation systems will increase the sensitivity of detection of virus shedding by considering the differences in growth and tropism of diverse SIV strains.</description><identifier>ISSN: 0830-9000</identifier><identifier>PMID: 11989733</identifier><language>eng</language><publisher>Canada</publisher><subject>accuracy ; Animals ; Cell Line - virology ; Chick Embryo - virology ; chicks ; culture techniques ; cultured cells ; disease diagnosis ; eggs ; Eggs - virology ; embryo (animal) ; Influenza A virus ; Influenza A virus - isolation & purification ; Nasal Cavity - virology ; Orthomyxoviridae Infections - diagnosis ; Orthomyxoviridae Infections - veterinary ; Sensitivity and Specificity ; Short Communication ; Specific Pathogen-Free Organisms ; strain differences ; Swine ; Swine Diseases - diagnosis ; Swine Diseases - virology</subject><ispartof>Canadian journal of veterinary research, 2002-04, Vol.66 (2), p.117-121</ispartof><rights>Copyright and/or publishing rights held by the Canadian Veterinary Medical Association</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC226993/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC226993/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11989733$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Clavijo, A</creatorcontrib><creatorcontrib>Tresnan, D.B</creatorcontrib><creatorcontrib>Jolie, R</creatorcontrib><creatorcontrib>Zhou, E.M</creatorcontrib><title>Comparison of embryonated chicken eggs with MDCK cell culture for the isolation of swine influenza virus</title><title>Canadian journal of veterinary research</title><addtitle>Can J Vet Res</addtitle><description>Embryonated chicken eggs (ECE) and the Madin-Darby canine kidney (MDCK) cell line were compared for isolation of swine influenza virus (SIV) from nasal swabs and tissue samples. Samples originated from 30 pigs experimentally inoculated with 2 x 106 to 2 x 10(7) embryo infectious dose 50% (EID50)/mL of swine influenza strain A/Swine/Indiana/1726/88 (H1N1). The results were analyzed with McNemar's chi-squared test for symmetry. The results indicated that more samples were SIV-positive with ECE than with tissue culture (P < 0.001), suggesting that ECE remains the system of choice for isolation of SIV. It is recommend that routine use of both SIV isolation systems will increase the sensitivity of detection of virus shedding by considering the differences in growth and tropism of diverse SIV strains.</description><subject>accuracy</subject><subject>Animals</subject><subject>Cell Line - virology</subject><subject>Chick Embryo - virology</subject><subject>chicks</subject><subject>culture techniques</subject><subject>cultured cells</subject><subject>disease diagnosis</subject><subject>eggs</subject><subject>Eggs - virology</subject><subject>embryo (animal)</subject><subject>Influenza A virus</subject><subject>Influenza A virus - isolation & purification</subject><subject>Nasal Cavity - virology</subject><subject>Orthomyxoviridae Infections - diagnosis</subject><subject>Orthomyxoviridae Infections - veterinary</subject><subject>Sensitivity and Specificity</subject><subject>Short Communication</subject><subject>Specific Pathogen-Free Organisms</subject><subject>strain differences</subject><subject>Swine</subject><subject>Swine Diseases - diagnosis</subject><subject>Swine Diseases - virology</subject><issn>0830-9000</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkEtPwzAQhHMA0VL4C-ATt0p-JbEPHFB4iiIO0LPlJHZiSOxiO63KryeoBcFppd1vZrRzkEwhI3DOIYST5DiENwhxnmFylEwQ4oznhEyTtnD9SnoTnAVOA9WXfuusjKoGVWuqd2WBapoANia24Om6eASV6jpQDV0cvALaeRBbBUZ9J6PZmYSNsePK6m5Q9lOCtfFDOEkOteyCOt3PWbK8vXkt7ueL57uH4mox15ilcZ7XihJU8RwpqXlZpjkmGYEKUURzSWhJeEWrmiINOaaaMKo5LBHXDDOYUkRmyeXOdzWUvaorZaOXnVh500u_FU4a8f9iTSsatxYYZ5yTUX-x13v3MagQRW_C98_SKjcEkaMsRZDDETz7G_Sb8NPtCJzvAC2dkM3YsVi-YIgIxBwzwhD5AoQQf5Y</recordid><startdate>20020401</startdate><enddate>20020401</enddate><creator>Clavijo, A</creator><creator>Tresnan, D.B</creator><creator>Jolie, R</creator><creator>Zhou, E.M</creator><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20020401</creationdate><title>Comparison of embryonated chicken eggs with MDCK cell culture for the isolation of swine influenza virus</title><author>Clavijo, A ; Tresnan, D.B ; Jolie, R ; Zhou, E.M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f285t-7de431c971eaf9bb5723630e14147a34b39c4cd41f0924f384f90b19f82805413</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>accuracy</topic><topic>Animals</topic><topic>Cell Line - virology</topic><topic>Chick Embryo - virology</topic><topic>chicks</topic><topic>culture techniques</topic><topic>cultured cells</topic><topic>disease diagnosis</topic><topic>eggs</topic><topic>Eggs - virology</topic><topic>embryo (animal)</topic><topic>Influenza A virus</topic><topic>Influenza A virus - isolation & purification</topic><topic>Nasal Cavity - virology</topic><topic>Orthomyxoviridae Infections - diagnosis</topic><topic>Orthomyxoviridae Infections - veterinary</topic><topic>Sensitivity and Specificity</topic><topic>Short Communication</topic><topic>Specific Pathogen-Free Organisms</topic><topic>strain differences</topic><topic>Swine</topic><topic>Swine Diseases - diagnosis</topic><topic>Swine Diseases - virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Clavijo, A</creatorcontrib><creatorcontrib>Tresnan, D.B</creatorcontrib><creatorcontrib>Jolie, R</creatorcontrib><creatorcontrib>Zhou, E.M</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Canadian journal of veterinary research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Clavijo, A</au><au>Tresnan, D.B</au><au>Jolie, R</au><au>Zhou, E.M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of embryonated chicken eggs with MDCK cell culture for the isolation of swine influenza virus</atitle><jtitle>Canadian journal of veterinary research</jtitle><addtitle>Can J Vet Res</addtitle><date>2002-04-01</date><risdate>2002</risdate><volume>66</volume><issue>2</issue><spage>117</spage><epage>121</epage><pages>117-121</pages><issn>0830-9000</issn><abstract>Embryonated chicken eggs (ECE) and the Madin-Darby canine kidney (MDCK) cell line were compared for isolation of swine influenza virus (SIV) from nasal swabs and tissue samples. Samples originated from 30 pigs experimentally inoculated with 2 x 106 to 2 x 10(7) embryo infectious dose 50% (EID50)/mL of swine influenza strain A/Swine/Indiana/1726/88 (H1N1). The results were analyzed with McNemar's chi-squared test for symmetry. The results indicated that more samples were SIV-positive with ECE than with tissue culture (P < 0.001), suggesting that ECE remains the system of choice for isolation of SIV. It is recommend that routine use of both SIV isolation systems will increase the sensitivity of detection of virus shedding by considering the differences in growth and tropism of diverse SIV strains.</abstract><cop>Canada</cop><pmid>11989733</pmid><tpages>5</tpages></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection |
| subjects | accuracy Animals Cell Line - virology Chick Embryo - virology chicks culture techniques cultured cells disease diagnosis eggs Eggs - virology embryo (animal) Influenza A virus Influenza A virus - isolation & purification Nasal Cavity - virology Orthomyxoviridae Infections - diagnosis Orthomyxoviridae Infections - veterinary Sensitivity and Specificity Short Communication Specific Pathogen-Free Organisms strain differences Swine Swine Diseases - diagnosis Swine Diseases - virology |
| title | Comparison of embryonated chicken eggs with MDCK cell culture for the isolation of swine influenza virus |
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