Mechanically gated channel activity in cytoskeleton-deficient plasma membrane blebs and vesicles from Xenopus oocytes

A novel technique involving hypertonic stress causes membrane âblebbingâ of the Xenopus oocyte and the shedding of plasma membrane vesicles (PMVs). Confocal fluorescence microscopy, immunocytochemistry and electron microscopy indicate that blebs and PMVs lack cortical cytoskeleton and are defici...

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Veröffentlicht in:	The Journal of physiology 2000-02, Vol.523 (1), p.117-130
Hauptverfasser:	Zhang, Yong, Gao, Feng, Popov, Vsevolod L., Wen, Julie W., Hamill, Owen P.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cell Membrane - physiology Cytoskeleton - physiology Electric Conductivity Female Fluorescent Antibody Technique Freshwater Hypertonic Solutions - pharmacology Ion Channel Gating Ion Channels - physiology Microscopy, Confocal Oocytes - drug effects Oocytes - physiology Original Space life sciences Xenopus Xenopus laevis - physiology
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