Calcium binding capacity of the cytosol and endoplasmic reticulum of mouse pancreatic acinar cells
The droplet technique was used in this study to measure total calcium loss from pancreatic acinar cells due to calcium extrusion. The calcium binding capacity of the cytosol ( k c ) was measured as the ratio of the decrease in the total calcium concentration of the cytosol of the cell (Î[Ca] c ) an...
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| Veröffentlicht in: | The Journal of physiology 1999-07, Vol.518 (2), p.463-467 |
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| creator | Mogami, Hideo Gardner, Julie Gerasimenko, Oleg V. Camello, Pedro Petersen, Ole H. Tepikin, Alexei V. |
| description | The droplet technique was used in this study to measure total calcium loss from pancreatic acinar cells due to calcium extrusion.
The calcium binding capacity of the cytosol ( k c ) was measured as the ratio of the decrease in the total calcium concentration of the cytosol of the cell (Î[Ca] c ) and the synchronously occurring decrease in the free calcium ion concentration in the cytosol (Î[Ca 2+ ] c ). The calcium dependency of the calcium binding capacity was determined by plotting values of k c against the corresponding [Ca 2+ ] c . The rise in the cytosolic Ca 2+ concentration of pancreatic acinar cells was triggered by stimulation with a supramaximal dose of cholecystokinin (CCK).
The recovery of [Ca 2+ ] c during continued exposure to the agonist was due to calcium extrusion from the cell.
The calcium binding capacity was about 1500-2000 for the [Ca 2+ ] c range 150-500 nM. The mechanism of buffering was not investigated in this study. The calcium binding capacity of the cytosol
did not vary significantly with [Ca 2+ ] c in this range. The CCK-evoked decrease in the total calcium concentration in the lumen of the endoplasmic reticulum (ER)
can be estimated from our data, taking into account previously published values for the volume of the ER in pancreatic acinar
cells. Comparing the decrease in the total ER calcium concentration with our recently reported values for agonist-induced
reductions in the free Ca 2+ concentration inside the ER, we estimate that the calcium binding capacity of the ER is approximately 20. In pancreatic acinar
cells we have therefore found a difference of two orders of magnitude in the efficiency of calcium buffering in the cytosol
and the ER lumen. |
| doi_str_mv | 10.1111/j.1469-7793.1999.0463p.x |
| format | Article |
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The calcium binding capacity of the cytosol ( k c ) was measured as the ratio of the decrease in the total calcium concentration of the cytosol of the cell (Î[Ca] c ) and the synchronously occurring decrease in the free calcium ion concentration in the cytosol (Î[Ca 2+ ] c ). The calcium dependency of the calcium binding capacity was determined by plotting values of k c against the corresponding [Ca 2+ ] c . The rise in the cytosolic Ca 2+ concentration of pancreatic acinar cells was triggered by stimulation with a supramaximal dose of cholecystokinin (CCK).
The recovery of [Ca 2+ ] c during continued exposure to the agonist was due to calcium extrusion from the cell.
The calcium binding capacity was about 1500-2000 for the [Ca 2+ ] c range 150-500 nM. The mechanism of buffering was not investigated in this study. The calcium binding capacity of the cytosol
did not vary significantly with [Ca 2+ ] c in this range. The CCK-evoked decrease in the total calcium concentration in the lumen of the endoplasmic reticulum (ER)
can be estimated from our data, taking into account previously published values for the volume of the ER in pancreatic acinar
cells. Comparing the decrease in the total ER calcium concentration with our recently reported values for agonist-induced
reductions in the free Ca 2+ concentration inside the ER, we estimate that the calcium binding capacity of the ER is approximately 20. In pancreatic acinar
cells we have therefore found a difference of two orders of magnitude in the efficiency of calcium buffering in the cytosol
and the ER lumen.</description><identifier>ISSN: 0022-3751</identifier><identifier>EISSN: 1469-7793</identifier><identifier>DOI: 10.1111/j.1469-7793.1999.0463p.x</identifier><identifier>PMID: 10381592</identifier><language>eng</language><publisher>Oxford, UK: The Physiological Society</publisher><subject>Algorithms ; Aniline Compounds ; Animals ; Calcium - metabolism ; Cholecystokinin - metabolism ; Cytosol - metabolism ; Endoplasmic Reticulum - metabolism ; Fluorescent Dyes ; Male ; Mice ; Pancreas - cytology ; Pancreas - metabolism ; Rapid Report ; Xanthenes</subject><ispartof>The Journal of physiology, 1999-07, Vol.518 (2), p.463-467</ispartof><rights>1999 The Journal of Physiology © 1999 The Physiological Society</rights><rights>The Physiological Society 1999 1999</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6003-86c5840bf13502a1b8691f8701382e754c76d36fb784f7260d8ba6c05e9aa3cf3</citedby><cites>FETCH-LOGICAL-c6003-86c5840bf13502a1b8691f8701382e754c76d36fb784f7260d8ba6c05e9aa3cf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2269443/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2269443/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,1417,1433,27924,27925,45574,45575,46409,46833,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10381592$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mogami, Hideo</creatorcontrib><creatorcontrib>Gardner, Julie</creatorcontrib><creatorcontrib>Gerasimenko, Oleg V.</creatorcontrib><creatorcontrib>Camello, Pedro</creatorcontrib><creatorcontrib>Petersen, Ole H.</creatorcontrib><creatorcontrib>Tepikin, Alexei V.</creatorcontrib><title>Calcium binding capacity of the cytosol and endoplasmic reticulum of mouse pancreatic acinar cells</title><title>The Journal of physiology</title><addtitle>J Physiol</addtitle><description>The droplet technique was used in this study to measure total calcium loss from pancreatic acinar cells due to calcium extrusion.
The calcium binding capacity of the cytosol ( k c ) was measured as the ratio of the decrease in the total calcium concentration of the cytosol of the cell (Î[Ca] c ) and the synchronously occurring decrease in the free calcium ion concentration in the cytosol (Î[Ca 2+ ] c ). The calcium dependency of the calcium binding capacity was determined by plotting values of k c against the corresponding [Ca 2+ ] c . The rise in the cytosolic Ca 2+ concentration of pancreatic acinar cells was triggered by stimulation with a supramaximal dose of cholecystokinin (CCK).
The recovery of [Ca 2+ ] c during continued exposure to the agonist was due to calcium extrusion from the cell.
The calcium binding capacity was about 1500-2000 for the [Ca 2+ ] c range 150-500 nM. The mechanism of buffering was not investigated in this study. The calcium binding capacity of the cytosol
did not vary significantly with [Ca 2+ ] c in this range. The CCK-evoked decrease in the total calcium concentration in the lumen of the endoplasmic reticulum (ER)
can be estimated from our data, taking into account previously published values for the volume of the ER in pancreatic acinar
cells. Comparing the decrease in the total ER calcium concentration with our recently reported values for agonist-induced
reductions in the free Ca 2+ concentration inside the ER, we estimate that the calcium binding capacity of the ER is approximately 20. In pancreatic acinar
cells we have therefore found a difference of two orders of magnitude in the efficiency of calcium buffering in the cytosol
and the ER lumen.</description><subject>Algorithms</subject><subject>Aniline Compounds</subject><subject>Animals</subject><subject>Calcium - metabolism</subject><subject>Cholecystokinin - metabolism</subject><subject>Cytosol - metabolism</subject><subject>Endoplasmic Reticulum - metabolism</subject><subject>Fluorescent Dyes</subject><subject>Male</subject><subject>Mice</subject><subject>Pancreas - cytology</subject><subject>Pancreas - metabolism</subject><subject>Rapid Report</subject><subject>Xanthenes</subject><issn>0022-3751</issn><issn>1469-7793</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUuL1TAYhoMozpnRvyBZqZvWXNpcQITh4HhhQBfj-iNN09McejPpcab_3tQOw7gRs0lInvclHw9CmJKcpvXumNNC6ExKzXOqtc5JIfiU3z1Bu4eHp2hHCGMZlyU9Q-cxHgmhnGj9HJ1RwhUtNduham866089rvxQ--GArZmM9fOCxwbPrcN2mcc4dtgMNXZDPU6dib23OLjZ21OXkgnsx1N0eDKDDc6ke5wqBhOwdV0XX6Bnjemie3m_X6AfVx9v9p-z62-fvuwvrzMrCOGZErZUBakaykvCDK2U0LRRMn1aMSfLwkpRc9FUUhWNZILUqjLCktJpY7ht-AX6sPVOp6p3tXXDHEwHU_C9CQuMxsPfL4Nv4TD-AsaELgqeCl7fF4Tx58nFGXof1xHM4NKAILQqZCllAt_-E6SyVKxgSUVC1YbaMMYYXPPwH0pgdQlHWJXBqgxWl_DHJdyl6KvH8zwKbvIS8H4Dbn3nlv8uhpuv39Mpxd9s8dYf2lsfHEztEn2ybb2bFyipAgYr-RvUpL2G</recordid><startdate>19990715</startdate><enddate>19990715</enddate><creator>Mogami, Hideo</creator><creator>Gardner, Julie</creator><creator>Gerasimenko, Oleg V.</creator><creator>Camello, Pedro</creator><creator>Petersen, Ole H.</creator><creator>Tepikin, Alexei V.</creator><general>The Physiological Society</general><general>Blackwell Science Ltd</general><general>Blackwell Science Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19990715</creationdate><title>Calcium binding capacity of the cytosol and endoplasmic reticulum of mouse pancreatic acinar cells</title><author>Mogami, Hideo ; Gardner, Julie ; Gerasimenko, Oleg V. ; Camello, Pedro ; Petersen, Ole H. ; Tepikin, Alexei V.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6003-86c5840bf13502a1b8691f8701382e754c76d36fb784f7260d8ba6c05e9aa3cf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Algorithms</topic><topic>Aniline Compounds</topic><topic>Animals</topic><topic>Calcium - metabolism</topic><topic>Cholecystokinin - metabolism</topic><topic>Cytosol - metabolism</topic><topic>Endoplasmic Reticulum - metabolism</topic><topic>Fluorescent Dyes</topic><topic>Male</topic><topic>Mice</topic><topic>Pancreas - cytology</topic><topic>Pancreas - metabolism</topic><topic>Rapid Report</topic><topic>Xanthenes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mogami, Hideo</creatorcontrib><creatorcontrib>Gardner, Julie</creatorcontrib><creatorcontrib>Gerasimenko, Oleg V.</creatorcontrib><creatorcontrib>Camello, Pedro</creatorcontrib><creatorcontrib>Petersen, Ole H.</creatorcontrib><creatorcontrib>Tepikin, Alexei V.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mogami, Hideo</au><au>Gardner, Julie</au><au>Gerasimenko, Oleg V.</au><au>Camello, Pedro</au><au>Petersen, Ole H.</au><au>Tepikin, Alexei V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Calcium binding capacity of the cytosol and endoplasmic reticulum of mouse pancreatic acinar cells</atitle><jtitle>The Journal of physiology</jtitle><addtitle>J Physiol</addtitle><date>1999-07-15</date><risdate>1999</risdate><volume>518</volume><issue>2</issue><spage>463</spage><epage>467</epage><pages>463-467</pages><issn>0022-3751</issn><eissn>1469-7793</eissn><abstract>The droplet technique was used in this study to measure total calcium loss from pancreatic acinar cells due to calcium extrusion.
The calcium binding capacity of the cytosol ( k c ) was measured as the ratio of the decrease in the total calcium concentration of the cytosol of the cell (Î[Ca] c ) and the synchronously occurring decrease in the free calcium ion concentration in the cytosol (Î[Ca 2+ ] c ). The calcium dependency of the calcium binding capacity was determined by plotting values of k c against the corresponding [Ca 2+ ] c . The rise in the cytosolic Ca 2+ concentration of pancreatic acinar cells was triggered by stimulation with a supramaximal dose of cholecystokinin (CCK).
The recovery of [Ca 2+ ] c during continued exposure to the agonist was due to calcium extrusion from the cell.
The calcium binding capacity was about 1500-2000 for the [Ca 2+ ] c range 150-500 nM. The mechanism of buffering was not investigated in this study. The calcium binding capacity of the cytosol
did not vary significantly with [Ca 2+ ] c in this range. The CCK-evoked decrease in the total calcium concentration in the lumen of the endoplasmic reticulum (ER)
can be estimated from our data, taking into account previously published values for the volume of the ER in pancreatic acinar
cells. Comparing the decrease in the total ER calcium concentration with our recently reported values for agonist-induced
reductions in the free Ca 2+ concentration inside the ER, we estimate that the calcium binding capacity of the ER is approximately 20. In pancreatic acinar
cells we have therefore found a difference of two orders of magnitude in the efficiency of calcium buffering in the cytosol
and the ER lumen.</abstract><cop>Oxford, UK</cop><pub>The Physiological Society</pub><pmid>10381592</pmid><doi>10.1111/j.1469-7793.1999.0463p.x</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Wiley Journals; Wiley Online Library Free Content; IngentaConnect Free/Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Algorithms Aniline Compounds Animals Calcium - metabolism Cholecystokinin - metabolism Cytosol - metabolism Endoplasmic Reticulum - metabolism Fluorescent Dyes Male Mice Pancreas - cytology Pancreas - metabolism Rapid Report Xanthenes |
| title | Calcium binding capacity of the cytosol and endoplasmic reticulum of mouse pancreatic acinar cells |
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