Identifying the site of spin trapping in proteins by a combination of liquid chromatography, ELISA, and off-line tandem mass spectrometry

An off-line mass spectrometry method that combines immuno-spin trapping and chromatographic procedures has been developed for selective detection of the nitrone spin trap 5,5-dimethyl-1-pyrroline- N-oxide (DMPO) covalently attached to proteins, an attachment which occurs only subsequent to DMPO trap...

Ausführliche Beschreibung