In vitro culture conditions to study keratinocyte differentiation using the HaCaT cell line
In vitro models to study the process of keratinocyte differentiation have been hindered by the stringent culture requirements and limitations imposed by the inherent properties of the cells. Primary keratinocytes only have a finite life span, while transformed cell lines exhibit many phenotypic feat...
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| Veröffentlicht in: | Cytotechnology (Dordrecht) 2007-06, Vol.54 (2), p.77-83 |
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| creator | DEYRIEUX, Adeline F WILSON, Van G |
| description | In vitro models to study the process of keratinocyte differentiation have been hindered by the stringent culture requirements and limitations imposed by the inherent properties of the cells. Primary keratinocytes only have a finite life span, while transformed cell lines exhibit many phenotypic features not found in normal cells. The spontaneously immortalized HaCaT cell line has been a widely employed keratinocyte model due to its ease of propagation and near normal phenotype, but protocols for differentiation and gene delivery into HaCaT cells vary widely in the literature. Here we report culture conditions for maintaining HaCaT cells in a basal-like state, for efficient differentiation of these cells, and for delivery of transgenes by transfection or adenoviral infection. This technological report will provide guidance to a large audience of scientists interested in investigating mechanisms of differentiation and skin morphogenesis. |
| doi_str_mv | 10.1007/s10616-007-9076-1 |
| format | Article |
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Primary keratinocytes only have a finite life span, while transformed cell lines exhibit many phenotypic features not found in normal cells. The spontaneously immortalized HaCaT cell line has been a widely employed keratinocyte model due to its ease of propagation and near normal phenotype, but protocols for differentiation and gene delivery into HaCaT cells vary widely in the literature. Here we report culture conditions for maintaining HaCaT cells in a basal-like state, for efficient differentiation of these cells, and for delivery of transgenes by transfection or adenoviral infection. 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Primary keratinocytes only have a finite life span, while transformed cell lines exhibit many phenotypic features not found in normal cells. The spontaneously immortalized HaCaT cell line has been a widely employed keratinocyte model due to its ease of propagation and near normal phenotype, but protocols for differentiation and gene delivery into HaCaT cells vary widely in the literature. Here we report culture conditions for maintaining HaCaT cells in a basal-like state, for efficient differentiation of these cells, and for delivery of transgenes by transfection or adenoviral infection. 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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Springer Nature - Complete Springer Journals; ProQuest Central UK/Ireland; PubMed Central; ProQuest Central |
| subjects | Biological and medical sciences Brief Report Cell culture Cell cycle Cell differentiation Cell lines Fundamental and applied biological sciences. Psychology Gene transfer Genotype & phenotype Infections Keratinocytes Microscopy Morphogenesis Morphology Phenotypes Propagation Transfection Transgenes |
| title | In vitro culture conditions to study keratinocyte differentiation using the HaCaT cell line |
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