Autoantibody profile in the experimental model of scleroderma induced by type V human collagen
The aim of this study is to evaluate the humoral autoimmune response in the experimental model of systemic sclerosis (SSc) induced by human type V collagen (huCol V). New Zealand rabbits were immunized with huCol V in Freund's complete adjuvant (FCA) and boosted twice with 15 days intervals wit...
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| Veröffentlicht in: | Immunology 2007-09, Vol.122 (1), p.38-46 |
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| creator | Callado, Maria R.M Viana, Vilma S.T Vendramini, Margarete B.G Leon, Elaine P Bueno, Cleonice Velosa, Ana P.P Teodoro, Walcy R Yoshinari, Natalino H |
| description | The aim of this study is to evaluate the humoral autoimmune response in the experimental model of systemic sclerosis (SSc) induced by human type V collagen (huCol V). New Zealand rabbits were immunized with huCol V in Freund's complete adjuvant (FCA) and boosted twice with 15 days intervals with huCol V in Freund's incomplete adjuvant. Control groups included animals injected only with FCA or bovine serum albumin. Bleeding was done at days 0, 30, 75 and 120. Tissue specimens were obtained for histopathological investigation. Serological analysis included detection of antibodies against huCol V and anti-topoisomerase I (Anti-Scl70) by enzyme-linked immunosorbent assay, antinuclear antibodies (ANA) by indirect immunofluorescence, and rheumatoid factor (RF) by a latex agglutination test. Target antigens were characterized by immunoblot. Histological analysis revealed extracellular matrix remodeling with fibrosis and vasculitis. Anti-Scl70 and ANA were detected as early as 30 days in all huCol V animals. The universal ANA staining pattern was Golgi-like. This serum reactivity was not abolished by previous absorption with huCol V. Characterization of the target antigen by immunoblot revealed two major protein fractions of 175 000 and 220 000 MW. Similarly to ANA, there was a gradual increase of reactivity throughout the immunization and also it was not abolished by preincubation of serum samples with huCol V. RF testing was negative in hyperimmune sera. Conclusion: The production of autoantibodies, including anti-Scl70, a serological marker for SSc associated with histopathological alterations, validates huCol V induced-experimental model and brings out its potential for understanding the pathophysiology of SSc. |
| doi_str_mv | 10.1111/j.1365-2567.2007.02610.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2265984</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>20423466</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5270-1dbb04561b7a0a11c12a83e9dd518a306bb8d586e50367c5f28d38ac8255d7283</originalsourceid><addsrcrecordid>eNqNks1u1DAUha0KRKctr1C8Ypepf2LHswCpqqCt1IoFLUssx76ZySiJg53A5O3rdEYFVuCNfXW_c3ytY4QwJUua1sV2SbkUGROyWDJCiiVhMvV2R2jx0niFFoTQVcYUEcfoJMZtKjkR4g06pkWeM8L4An2_HAdvuqEuvZtwH3xVN4DrDg8bwLDrIdQtdINpcOsdNNhXONoGQipCaxLoRgsOlxMeph7wN7wZW9Nh65vGrKE7Q68r00R4e9hP0ePnTw9XN9ndl-vbq8u7zApWkIy6siS5kLQsDDGUWsqM4rByTlBlOJFlqZxQEgThsrCiYspxZaxiQriCKX6KPu59-7Fswdk0cjCN7tP0Jkzam1r_3enqjV77n5oxKVYqTwbvDwbB_xghDrqto4X0ig78GLVUNJdSqX-CjOSMJzSBag_a4GMMUL1MQ4meU9RbPYel57D0nKJ-TlHvkvT8z9f8Fh5iS8CHPfArpTX9t7G-vb-fT0n_bq-vjNdmHeqoH7-y-XuQYiVluuEJs2e1tg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>20423466</pqid></control><display><type>article</type><title>Autoantibody profile in the experimental model of scleroderma induced by type V human collagen</title><source>Wiley Online Library - AutoHoldings Journals</source><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>IngentaConnect Free/Open Access Journals</source><source>Wiley Online Library (Open Access Collection)</source><source>PubMed Central</source><creator>Callado, Maria R.M ; Viana, Vilma S.T ; Vendramini, Margarete B.G ; Leon, Elaine P ; Bueno, Cleonice ; Velosa, Ana P.P ; Teodoro, Walcy R ; Yoshinari, Natalino H</creator><creatorcontrib>Callado, Maria R.M ; Viana, Vilma S.T ; Vendramini, Margarete B.G ; Leon, Elaine P ; Bueno, Cleonice ; Velosa, Ana P.P ; Teodoro, Walcy R ; Yoshinari, Natalino H</creatorcontrib><description>The aim of this study is to evaluate the humoral autoimmune response in the experimental model of systemic sclerosis (SSc) induced by human type V collagen (huCol V). New Zealand rabbits were immunized with huCol V in Freund's complete adjuvant (FCA) and boosted twice with 15 days intervals with huCol V in Freund's incomplete adjuvant. Control groups included animals injected only with FCA or bovine serum albumin. Bleeding was done at days 0, 30, 75 and 120. Tissue specimens were obtained for histopathological investigation. Serological analysis included detection of antibodies against huCol V and anti-topoisomerase I (Anti-Scl70) by enzyme-linked immunosorbent assay, antinuclear antibodies (ANA) by indirect immunofluorescence, and rheumatoid factor (RF) by a latex agglutination test. Target antigens were characterized by immunoblot. Histological analysis revealed extracellular matrix remodeling with fibrosis and vasculitis. Anti-Scl70 and ANA were detected as early as 30 days in all huCol V animals. The universal ANA staining pattern was Golgi-like. This serum reactivity was not abolished by previous absorption with huCol V. Characterization of the target antigen by immunoblot revealed two major protein fractions of 175 000 and 220 000 MW. Similarly to ANA, there was a gradual increase of reactivity throughout the immunization and also it was not abolished by preincubation of serum samples with huCol V. RF testing was negative in hyperimmune sera. Conclusion: The production of autoantibodies, including anti-Scl70, a serological marker for SSc associated with histopathological alterations, validates huCol V induced-experimental model and brings out its potential for understanding the pathophysiology of SSc.</description><identifier>ISSN: 0019-2805</identifier><identifier>EISSN: 1365-2567</identifier><identifier>DOI: 10.1111/j.1365-2567.2007.02610.x</identifier><identifier>PMID: 17442023</identifier><language>eng</language><publisher>Oxford, UK: Oxford, UK : Blackwell Publishing Ltd</publisher><subject>Animals ; Antibodies, Antinuclear - blood ; autoantibodies ; Autoantibodies - biosynthesis ; Biomarkers - blood ; Collagen Type V - immunology ; Disease Models, Animal ; DNA Topoisomerases, Type I - immunology ; Enzyme-Linked Immunosorbent Assay - methods ; Esophagus - pathology ; experimental model ; Female ; Humans ; Immunization - methods ; Lung - pathology ; Original ; Rabbits ; Rheumatoid Factor - blood ; Scleroderma ; Scleroderma, Systemic - immunology ; Scleroderma, Systemic - pathology ; Skin - pathology ; systemic sclerosis ; Type V collagen</subject><ispartof>Immunology, 2007-09, Vol.122 (1), p.38-46</ispartof><rights>2007 Blackwell Publishing Ltd 2007</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5270-1dbb04561b7a0a11c12a83e9dd518a306bb8d586e50367c5f28d38ac8255d7283</citedby><cites>FETCH-LOGICAL-c5270-1dbb04561b7a0a11c12a83e9dd518a306bb8d586e50367c5f28d38ac8255d7283</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2265984/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2265984/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,315,728,781,785,886,1418,1434,27929,27930,45579,45580,46414,46838,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17442023$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Callado, Maria R.M</creatorcontrib><creatorcontrib>Viana, Vilma S.T</creatorcontrib><creatorcontrib>Vendramini, Margarete B.G</creatorcontrib><creatorcontrib>Leon, Elaine P</creatorcontrib><creatorcontrib>Bueno, Cleonice</creatorcontrib><creatorcontrib>Velosa, Ana P.P</creatorcontrib><creatorcontrib>Teodoro, Walcy R</creatorcontrib><creatorcontrib>Yoshinari, Natalino H</creatorcontrib><title>Autoantibody profile in the experimental model of scleroderma induced by type V human collagen</title><title>Immunology</title><addtitle>Immunology</addtitle><description>The aim of this study is to evaluate the humoral autoimmune response in the experimental model of systemic sclerosis (SSc) induced by human type V collagen (huCol V). New Zealand rabbits were immunized with huCol V in Freund's complete adjuvant (FCA) and boosted twice with 15 days intervals with huCol V in Freund's incomplete adjuvant. Control groups included animals injected only with FCA or bovine serum albumin. Bleeding was done at days 0, 30, 75 and 120. Tissue specimens were obtained for histopathological investigation. Serological analysis included detection of antibodies against huCol V and anti-topoisomerase I (Anti-Scl70) by enzyme-linked immunosorbent assay, antinuclear antibodies (ANA) by indirect immunofluorescence, and rheumatoid factor (RF) by a latex agglutination test. Target antigens were characterized by immunoblot. Histological analysis revealed extracellular matrix remodeling with fibrosis and vasculitis. Anti-Scl70 and ANA were detected as early as 30 days in all huCol V animals. The universal ANA staining pattern was Golgi-like. This serum reactivity was not abolished by previous absorption with huCol V. Characterization of the target antigen by immunoblot revealed two major protein fractions of 175 000 and 220 000 MW. Similarly to ANA, there was a gradual increase of reactivity throughout the immunization and also it was not abolished by preincubation of serum samples with huCol V. RF testing was negative in hyperimmune sera. Conclusion: The production of autoantibodies, including anti-Scl70, a serological marker for SSc associated with histopathological alterations, validates huCol V induced-experimental model and brings out its potential for understanding the pathophysiology of SSc.</description><subject>Animals</subject><subject>Antibodies, Antinuclear - blood</subject><subject>autoantibodies</subject><subject>Autoantibodies - biosynthesis</subject><subject>Biomarkers - blood</subject><subject>Collagen Type V - immunology</subject><subject>Disease Models, Animal</subject><subject>DNA Topoisomerases, Type I - immunology</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Esophagus - pathology</subject><subject>experimental model</subject><subject>Female</subject><subject>Humans</subject><subject>Immunization - methods</subject><subject>Lung - pathology</subject><subject>Original</subject><subject>Rabbits</subject><subject>Rheumatoid Factor - blood</subject><subject>Scleroderma</subject><subject>Scleroderma, Systemic - immunology</subject><subject>Scleroderma, Systemic - pathology</subject><subject>Skin - pathology</subject><subject>systemic sclerosis</subject><subject>Type V collagen</subject><issn>0019-2805</issn><issn>1365-2567</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNks1u1DAUha0KRKctr1C8Ypepf2LHswCpqqCt1IoFLUssx76ZySiJg53A5O3rdEYFVuCNfXW_c3ytY4QwJUua1sV2SbkUGROyWDJCiiVhMvV2R2jx0niFFoTQVcYUEcfoJMZtKjkR4g06pkWeM8L4An2_HAdvuqEuvZtwH3xVN4DrDg8bwLDrIdQtdINpcOsdNNhXONoGQipCaxLoRgsOlxMeph7wN7wZW9Nh65vGrKE7Q68r00R4e9hP0ePnTw9XN9ndl-vbq8u7zApWkIy6siS5kLQsDDGUWsqM4rByTlBlOJFlqZxQEgThsrCiYspxZaxiQriCKX6KPu59-7Fswdk0cjCN7tP0Jkzam1r_3enqjV77n5oxKVYqTwbvDwbB_xghDrqto4X0ig78GLVUNJdSqX-CjOSMJzSBag_a4GMMUL1MQ4meU9RbPYel57D0nKJ-TlHvkvT8z9f8Fh5iS8CHPfArpTX9t7G-vb-fT0n_bq-vjNdmHeqoH7-y-XuQYiVluuEJs2e1tg</recordid><startdate>200709</startdate><enddate>200709</enddate><creator>Callado, Maria R.M</creator><creator>Viana, Vilma S.T</creator><creator>Vendramini, Margarete B.G</creator><creator>Leon, Elaine P</creator><creator>Bueno, Cleonice</creator><creator>Velosa, Ana P.P</creator><creator>Teodoro, Walcy R</creator><creator>Yoshinari, Natalino H</creator><general>Oxford, UK : Blackwell Publishing Ltd</general><general>Blackwell Publishing Ltd</general><general>Blackwell Science Inc</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>200709</creationdate><title>Autoantibody profile in the experimental model of scleroderma induced by type V human collagen</title><author>Callado, Maria R.M ; Viana, Vilma S.T ; Vendramini, Margarete B.G ; Leon, Elaine P ; Bueno, Cleonice ; Velosa, Ana P.P ; Teodoro, Walcy R ; Yoshinari, Natalino H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5270-1dbb04561b7a0a11c12a83e9dd518a306bb8d586e50367c5f28d38ac8255d7283</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Antibodies, Antinuclear - blood</topic><topic>autoantibodies</topic><topic>Autoantibodies - biosynthesis</topic><topic>Biomarkers - blood</topic><topic>Collagen Type V - immunology</topic><topic>Disease Models, Animal</topic><topic>DNA Topoisomerases, Type I - immunology</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Esophagus - pathology</topic><topic>experimental model</topic><topic>Female</topic><topic>Humans</topic><topic>Immunization - methods</topic><topic>Lung - pathology</topic><topic>Original</topic><topic>Rabbits</topic><topic>Rheumatoid Factor - blood</topic><topic>Scleroderma</topic><topic>Scleroderma, Systemic - immunology</topic><topic>Scleroderma, Systemic - pathology</topic><topic>Skin - pathology</topic><topic>systemic sclerosis</topic><topic>Type V collagen</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Callado, Maria R.M</creatorcontrib><creatorcontrib>Viana, Vilma S.T</creatorcontrib><creatorcontrib>Vendramini, Margarete B.G</creatorcontrib><creatorcontrib>Leon, Elaine P</creatorcontrib><creatorcontrib>Bueno, Cleonice</creatorcontrib><creatorcontrib>Velosa, Ana P.P</creatorcontrib><creatorcontrib>Teodoro, Walcy R</creatorcontrib><creatorcontrib>Yoshinari, Natalino H</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Callado, Maria R.M</au><au>Viana, Vilma S.T</au><au>Vendramini, Margarete B.G</au><au>Leon, Elaine P</au><au>Bueno, Cleonice</au><au>Velosa, Ana P.P</au><au>Teodoro, Walcy R</au><au>Yoshinari, Natalino H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Autoantibody profile in the experimental model of scleroderma induced by type V human collagen</atitle><jtitle>Immunology</jtitle><addtitle>Immunology</addtitle><date>2007-09</date><risdate>2007</risdate><volume>122</volume><issue>1</issue><spage>38</spage><epage>46</epage><pages>38-46</pages><issn>0019-2805</issn><eissn>1365-2567</eissn><abstract>The aim of this study is to evaluate the humoral autoimmune response in the experimental model of systemic sclerosis (SSc) induced by human type V collagen (huCol V). New Zealand rabbits were immunized with huCol V in Freund's complete adjuvant (FCA) and boosted twice with 15 days intervals with huCol V in Freund's incomplete adjuvant. Control groups included animals injected only with FCA or bovine serum albumin. Bleeding was done at days 0, 30, 75 and 120. Tissue specimens were obtained for histopathological investigation. Serological analysis included detection of antibodies against huCol V and anti-topoisomerase I (Anti-Scl70) by enzyme-linked immunosorbent assay, antinuclear antibodies (ANA) by indirect immunofluorescence, and rheumatoid factor (RF) by a latex agglutination test. Target antigens were characterized by immunoblot. Histological analysis revealed extracellular matrix remodeling with fibrosis and vasculitis. Anti-Scl70 and ANA were detected as early as 30 days in all huCol V animals. The universal ANA staining pattern was Golgi-like. This serum reactivity was not abolished by previous absorption with huCol V. Characterization of the target antigen by immunoblot revealed two major protein fractions of 175 000 and 220 000 MW. Similarly to ANA, there was a gradual increase of reactivity throughout the immunization and also it was not abolished by preincubation of serum samples with huCol V. RF testing was negative in hyperimmune sera. Conclusion: The production of autoantibodies, including anti-Scl70, a serological marker for SSc associated with histopathological alterations, validates huCol V induced-experimental model and brings out its potential for understanding the pathophysiology of SSc.</abstract><cop>Oxford, UK</cop><pub>Oxford, UK : Blackwell Publishing Ltd</pub><pmid>17442023</pmid><doi>10.1111/j.1365-2567.2007.02610.x</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Antibodies, Antinuclear - blood autoantibodies Autoantibodies - biosynthesis Biomarkers - blood Collagen Type V - immunology Disease Models, Animal DNA Topoisomerases, Type I - immunology Enzyme-Linked Immunosorbent Assay - methods Esophagus - pathology experimental model Female Humans Immunization - methods Lung - pathology Original Rabbits Rheumatoid Factor - blood Scleroderma Scleroderma, Systemic - immunology Scleroderma, Systemic - pathology Skin - pathology systemic sclerosis Type V collagen |
| title | Autoantibody profile in the experimental model of scleroderma induced by type V human collagen |
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