Functional expression of eukaryotic membrane proteins in Lactococcus lactis

The overproduction of eukaryotic membrane proteins is a major impediment in their structural and functional characterization. Here we have used the nisin‐inducible expression system of Lactococcus lactis for the overproduction of 11 mitochondrial transport proteins from yeast. They were expressed at...

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Veröffentlicht in:	Protein science 2005-12, Vol.14 (12), p.3048-3056
Hauptverfasser:	Monné, Magnus, Chan, Ka Wai, Slotboom, Dirk‐Jan, Kunji, Edmund R.S.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Carrier Proteins - biosynthesis Carrier Proteins - chemistry Carrier Proteins - genetics Carrier Proteins - metabolism eukaryotic membrane proteins Gene Expression Lactococcus lactis Lactococcus lactis - genetics Membrane Proteins - biosynthesis Membrane Proteins - chemistry Membrane Proteins - genetics Membrane Proteins - metabolism Mitochondria - metabolism mitochondrial carriers Mitochondrial Proteins - biosynthesis Mitochondrial Proteins - chemistry Mitochondrial Proteins - genetics Mitochondrial Proteins - metabolism Molecular Sequence Data nisin‐inducible expression system NMR. nuclear magnetic resonance Protein Sorting Signals - genetics Protein Sorting Signals - physiology Recombinant Proteins - chemistry Recombinant Proteins - genetics Recombinant Proteins - metabolism Saccharomyces cerevisiae - chemistry Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism Saccharomyces cerevisiae Proteins - biosynthesis Saccharomyces cerevisiae Proteins - chemistry Saccharomyces cerevisiae Proteins - genetics Saccharomyces cerevisiae Proteins - metabolism TIM, translocase of the inner membrane TOM, translo‐case of the outer membrane transport
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