Undistorted structural analysis of soluble proteins by attenuated total reflectance infrared spectroscopy

Water from the solvent very strongly absorbs light in the frequency range of interest for studying protein structure by infrared (IR) spectroscopy. This renders handling of the observation cells painstaking and time consuming, and limits the reproducibility of the measurements when IR spectroscopy i...

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Veröffentlicht in:	Protein science 2005-11, Vol.14 (11), p.2781-2792
Hauptverfasser:	Goldberg, Michel E., Chaffotte, Alain F.
Format:	Artikel
Sprache:	eng
Schlagworte:	adsorption ATR ATR, attenuated total reflectance Biochemistry, Molecular Biology FKPA, a peptidylprolyl cis–trans isomerase from Escherichia coli FTIR FTIR, Fourier transform infrared Life Sciences MBP, the maltose binding protein from Escherichia coli Muramidase Muramidase - chemistry PDB, the Research Collaboratory for Structural Bioinformatics (RCSB) Protein Data Bank Protein Structure, Secondary proteins Reproducibility of Results secondary structure Solubility Solutions Spectroscopy, Fourier Transform Infrared Spectroscopy, Fourier Transform Infrared - methods Structural Biology TRX, the oxidized thioredoxin from Escherichia coli
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