S100A8 Triggers Oxidation-sensitive Repulsion of Neutrophils

The inflammatory response to tissue injury is a multi-faceted process. During this process, neutrophils migrate in the extravascular spaces, directed to the site of injury by chemical gradients generated by chemotactic molecules. S100A8, a protein associated with a wide variety of inflammatory condi...

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Veröffentlicht in:Journal of dental research 2006-09, Vol.85 (9), p.829-833
Hauptverfasser: Sroussi, H.Y., Berline, J., Dazin, P., Green, P., Palefsky, J.M.
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container_end_page 833
container_issue 9
container_start_page 829
container_title Journal of dental research
container_volume 85
creator Sroussi, H.Y.
Berline, J.
Dazin, P.
Green, P.
Palefsky, J.M.
description The inflammatory response to tissue injury is a multi-faceted process. During this process, neutrophils migrate in the extravascular spaces, directed to the site of injury by chemical gradients generated by chemotactic molecules. S100A8, a protein associated with a wide variety of inflammatory conditions, is heavily over-expressed in association with inflammation. We hypothesized that human S100A8 possesses neutrophil-repelling properties that result in an anti-inflammatory effect in vivo. The chemotactic activity of S100A8 on neutrophils was tested in Transwell chemotaxis assays. Analysis of the data indicates that S100A8 causes a repulsion of peripheral neutrophils, an activity that S100A8 loses upon its oxidation. Using a mutant of S100A8 resistant to oxidation and consistent with the in vitro findings, we demonstrated that S100A8 causes a strong anti-inflammatory effect in the rat air-pouch model of inflammation in vivo. These data highlight a naturally occurring novel anti-inflammatory pathway and provide potential molecular targets for the development of novel anti-inflammatory therapeutics. Abbrevations: ethylene diamine tetraacetic acid (EDTA); limulus amoebocyte lysate assay (LAL); pertussis toxin (PTX); forward scatter (FSC); Interleukin-8 (IL-8); formyl-Met-Leu-Phe (fMLP); monocyte chemotactic protein 1 (MCP1).
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These data highlight a naturally occurring novel anti-inflammatory pathway and provide potential molecular targets for the development of novel anti-inflammatory therapeutics. 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subjects Alanine - metabolism
Animals
Calgranulin A - physiology
Cells, Cultured
Chemotaxis, Leukocyte - drug effects
Chemotaxis, Leukocyte - physiology
Cysteine - metabolism
Dentistry
Flow Cytometry
Humans
Inflammation - metabolism
Inflammation Mediators - antagonists & inhibitors
Lipopolysaccharides
N-Formylmethionine Leucyl-Phenylalanine - antagonists & inhibitors
Neutrophils - drug effects
Neutrophils - physiology
Oxidation-Reduction
Rats
Recombinant Proteins - pharmacology
title S100A8 Triggers Oxidation-sensitive Repulsion of Neutrophils
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