Competition between the ATPase Prp5 and Branch Region-U2 snRNA Pairing Modulates the Fidelity of Spliceosome Assembly

ATPase-facilitated steps during spliceosome function have been postulated to afford opportunities for kinetic proofreading. Spliceosome assembly requires the ATPase Prp5p, whose activity might thus impact fidelity during initial intron recognition. Using alanine mutations in S. cerevisiae Prp5p, we...

Ausführliche Beschreibung