In vivo airway surface liquid Cl- analysis with solid-state electrodes

The pathogenesis of cystic fibrosis (CF) airways disease remains controversial. Hypotheses that link mutations in CFTR and defects in ion transport to CF lung disease predict that alterations in airway surface liquid (ASL) isotonic volume, or ion composition, are critically important. ASL [Cl-] is p...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:The Journal of general physiology 2002-01, Vol.119 (1), p.3-14
Hauptverfasser: Caldwell, Ray A, Grubb, Barbara R, Tarran, Robert, Boucher, Richard C, Knowles, Michael R, Barker, Pierre M
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 14
container_issue 1
container_start_page 3
container_title The Journal of general physiology
container_volume 119
creator Caldwell, Ray A
Grubb, Barbara R
Tarran, Robert
Boucher, Richard C
Knowles, Michael R
Barker, Pierre M
description The pathogenesis of cystic fibrosis (CF) airways disease remains controversial. Hypotheses that link mutations in CFTR and defects in ion transport to CF lung disease predict that alterations in airway surface liquid (ASL) isotonic volume, or ion composition, are critically important. ASL [Cl-] is pivotal in discriminating between these hypotheses, but there is no consensus on this value given the difficulty in measuring [Cl-] in the "thin" ASL (approximately 30 microm) in vivo. Consequently, a miniaturized solid-state electrode with a shallow depth of immersion was constructed to measure ASL [Cl-] in vivo. In initial experiments, the electrode measured [Cl-] in physiologic salt solutions, small volume (7.6 microl) test solutions, and in in vitro cell culture models, with > or =93% accuracy. Based on discrepancies in reported values and/or absence of data, ASL Cl- measurements were made in the following airway regions and species. First, ASL [Cl-] was measured in normal human nasal cavity and averaged 117.3 +/- 11.2 mM (n = 6). Second, ASL [Cl-] measured in large airway (tracheobronchial) regions were as follows: rabbit trachea and bronchus = 114.3 +/- 1.8 mM; (n = 6) and 126.9 +/- 1.7 mM; (n = 3), respectively; mouse trachea = 112.8 +/- 4.2 mM (n = 13); and monkey bronchus = 112.3 +/- 10.9 mM (n = 3). Third, Cl- measurements were made in small (1-2 mm) diameter airways of the rabbit (108.3 +/- 7.1 mM, n = 5) and monkey (128.5 +/- 6.8 mM, n = 3). The measured [Cl-], in excess of 100 mM throughout all airway regions tested in multiple species, is consistent with the isotonic volume hypothesis to describe ASL physiology.
doi_str_mv 10.1085/jgp.119.1.3
format Article
fullrecord <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2233861</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>71356155</sourcerecordid><originalsourceid>FETCH-LOGICAL-c377t-ff4846c908fce3d5643e5ff7e967c4ee886ef6ee532e230af59c8aaad00bfd533</originalsourceid><addsrcrecordid>eNpVkD1PwzAQhi0EoqUwsSNPLCjBH3GcLEioolCpEgvMluucW1duUuykVf89qVrxccsN9-i9Vw9Ct5SklBTicbXYpJSWKU35GRpSkZFEyqw4R0NCGEsoK8UAXcW4Iv0IRi7RgFIpOePZEE2mNd66bYO1Czu9x7ELVhvA3n11rsJjn2Bda7-PLuKda5c4Nt5VSWx1Cxg8mDY0FcRrdGG1j3Bz2iP0OXn5GL8ls_fX6fh5lhguZZtYmxVZbkpSWAO8EnnGQVgrocylyQCKIgebAwjOgHGirShNobWuCJnbSnA-Qk_H3E03X0NloG6D9moT3FqHvWq0U_8vtVuqRbNVjHFe5LQPuD8FhOarg9iqtYsGvNc1NF1UknKRUyF68OEImtDEGMD-PKFEHbyr3rvqvSuqDr3u_vb6ZU-i-TdWmICS</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>71356155</pqid></control><display><type>article</type><title>In vivo airway surface liquid Cl- analysis with solid-state electrodes</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Caldwell, Ray A ; Grubb, Barbara R ; Tarran, Robert ; Boucher, Richard C ; Knowles, Michael R ; Barker, Pierre M</creator><creatorcontrib>Caldwell, Ray A ; Grubb, Barbara R ; Tarran, Robert ; Boucher, Richard C ; Knowles, Michael R ; Barker, Pierre M</creatorcontrib><description>The pathogenesis of cystic fibrosis (CF) airways disease remains controversial. Hypotheses that link mutations in CFTR and defects in ion transport to CF lung disease predict that alterations in airway surface liquid (ASL) isotonic volume, or ion composition, are critically important. ASL [Cl-] is pivotal in discriminating between these hypotheses, but there is no consensus on this value given the difficulty in measuring [Cl-] in the "thin" ASL (approximately 30 microm) in vivo. Consequently, a miniaturized solid-state electrode with a shallow depth of immersion was constructed to measure ASL [Cl-] in vivo. In initial experiments, the electrode measured [Cl-] in physiologic salt solutions, small volume (7.6 microl) test solutions, and in in vitro cell culture models, with &gt; or =93% accuracy. Based on discrepancies in reported values and/or absence of data, ASL Cl- measurements were made in the following airway regions and species. First, ASL [Cl-] was measured in normal human nasal cavity and averaged 117.3 +/- 11.2 mM (n = 6). Second, ASL [Cl-] measured in large airway (tracheobronchial) regions were as follows: rabbit trachea and bronchus = 114.3 +/- 1.8 mM; (n = 6) and 126.9 +/- 1.7 mM; (n = 3), respectively; mouse trachea = 112.8 +/- 4.2 mM (n = 13); and monkey bronchus = 112.3 +/- 10.9 mM (n = 3). Third, Cl- measurements were made in small (1-2 mm) diameter airways of the rabbit (108.3 +/- 7.1 mM, n = 5) and monkey (128.5 +/- 6.8 mM, n = 3). The measured [Cl-], in excess of 100 mM throughout all airway regions tested in multiple species, is consistent with the isotonic volume hypothesis to describe ASL physiology.</description><identifier>ISSN: 0022-1295</identifier><identifier>EISSN: 1540-7748</identifier><identifier>DOI: 10.1085/jgp.119.1.3</identifier><identifier>PMID: 11773234</identifier><language>eng</language><publisher>United States: The Rockefeller University Press</publisher><subject>Adult ; Animals ; Bronchi - chemistry ; Bronchi - physiology ; Chlorides - analysis ; Chlorides - standards ; Female ; Humans ; Ion-Selective Electrodes - standards ; Macaca mulatta ; Male ; Mice ; Mice, Inbred C57BL ; Middle Aged ; Nasal Cavity - chemistry ; Nasal Cavity - physiology ; Original ; Rabbits ; Solutions ; Trachea - chemistry ; Trachea - physiology</subject><ispartof>The Journal of general physiology, 2002-01, Vol.119 (1), p.3-14</ispartof><rights>2002 The Rockefeller University Press 2002 The Rockefeller University Press</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c377t-ff4846c908fce3d5643e5ff7e967c4ee886ef6ee532e230af59c8aaad00bfd533</citedby><cites>FETCH-LOGICAL-c377t-ff4846c908fce3d5643e5ff7e967c4ee886ef6ee532e230af59c8aaad00bfd533</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2233861/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2233861/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,729,782,786,887,27931,27932,53798,53800</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11773234$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Caldwell, Ray A</creatorcontrib><creatorcontrib>Grubb, Barbara R</creatorcontrib><creatorcontrib>Tarran, Robert</creatorcontrib><creatorcontrib>Boucher, Richard C</creatorcontrib><creatorcontrib>Knowles, Michael R</creatorcontrib><creatorcontrib>Barker, Pierre M</creatorcontrib><title>In vivo airway surface liquid Cl- analysis with solid-state electrodes</title><title>The Journal of general physiology</title><addtitle>J Gen Physiol</addtitle><description>The pathogenesis of cystic fibrosis (CF) airways disease remains controversial. Hypotheses that link mutations in CFTR and defects in ion transport to CF lung disease predict that alterations in airway surface liquid (ASL) isotonic volume, or ion composition, are critically important. ASL [Cl-] is pivotal in discriminating between these hypotheses, but there is no consensus on this value given the difficulty in measuring [Cl-] in the "thin" ASL (approximately 30 microm) in vivo. Consequently, a miniaturized solid-state electrode with a shallow depth of immersion was constructed to measure ASL [Cl-] in vivo. In initial experiments, the electrode measured [Cl-] in physiologic salt solutions, small volume (7.6 microl) test solutions, and in in vitro cell culture models, with &gt; or =93% accuracy. Based on discrepancies in reported values and/or absence of data, ASL Cl- measurements were made in the following airway regions and species. First, ASL [Cl-] was measured in normal human nasal cavity and averaged 117.3 +/- 11.2 mM (n = 6). Second, ASL [Cl-] measured in large airway (tracheobronchial) regions were as follows: rabbit trachea and bronchus = 114.3 +/- 1.8 mM; (n = 6) and 126.9 +/- 1.7 mM; (n = 3), respectively; mouse trachea = 112.8 +/- 4.2 mM (n = 13); and monkey bronchus = 112.3 +/- 10.9 mM (n = 3). Third, Cl- measurements were made in small (1-2 mm) diameter airways of the rabbit (108.3 +/- 7.1 mM, n = 5) and monkey (128.5 +/- 6.8 mM, n = 3). The measured [Cl-], in excess of 100 mM throughout all airway regions tested in multiple species, is consistent with the isotonic volume hypothesis to describe ASL physiology.</description><subject>Adult</subject><subject>Animals</subject><subject>Bronchi - chemistry</subject><subject>Bronchi - physiology</subject><subject>Chlorides - analysis</subject><subject>Chlorides - standards</subject><subject>Female</subject><subject>Humans</subject><subject>Ion-Selective Electrodes - standards</subject><subject>Macaca mulatta</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Middle Aged</subject><subject>Nasal Cavity - chemistry</subject><subject>Nasal Cavity - physiology</subject><subject>Original</subject><subject>Rabbits</subject><subject>Solutions</subject><subject>Trachea - chemistry</subject><subject>Trachea - physiology</subject><issn>0022-1295</issn><issn>1540-7748</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkD1PwzAQhi0EoqUwsSNPLCjBH3GcLEioolCpEgvMluucW1duUuykVf89qVrxccsN9-i9Vw9Ct5SklBTicbXYpJSWKU35GRpSkZFEyqw4R0NCGEsoK8UAXcW4Iv0IRi7RgFIpOePZEE2mNd66bYO1Czu9x7ELVhvA3n11rsJjn2Bda7-PLuKda5c4Nt5VSWx1Cxg8mDY0FcRrdGG1j3Bz2iP0OXn5GL8ls_fX6fh5lhguZZtYmxVZbkpSWAO8EnnGQVgrocylyQCKIgebAwjOgHGirShNobWuCJnbSnA-Qk_H3E03X0NloG6D9moT3FqHvWq0U_8vtVuqRbNVjHFe5LQPuD8FhOarg9iqtYsGvNc1NF1UknKRUyF68OEImtDEGMD-PKFEHbyr3rvqvSuqDr3u_vb6ZU-i-TdWmICS</recordid><startdate>200201</startdate><enddate>200201</enddate><creator>Caldwell, Ray A</creator><creator>Grubb, Barbara R</creator><creator>Tarran, Robert</creator><creator>Boucher, Richard C</creator><creator>Knowles, Michael R</creator><creator>Barker, Pierre M</creator><general>The Rockefeller University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>200201</creationdate><title>In vivo airway surface liquid Cl- analysis with solid-state electrodes</title><author>Caldwell, Ray A ; Grubb, Barbara R ; Tarran, Robert ; Boucher, Richard C ; Knowles, Michael R ; Barker, Pierre M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c377t-ff4846c908fce3d5643e5ff7e967c4ee886ef6ee532e230af59c8aaad00bfd533</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Adult</topic><topic>Animals</topic><topic>Bronchi - chemistry</topic><topic>Bronchi - physiology</topic><topic>Chlorides - analysis</topic><topic>Chlorides - standards</topic><topic>Female</topic><topic>Humans</topic><topic>Ion-Selective Electrodes - standards</topic><topic>Macaca mulatta</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Middle Aged</topic><topic>Nasal Cavity - chemistry</topic><topic>Nasal Cavity - physiology</topic><topic>Original</topic><topic>Rabbits</topic><topic>Solutions</topic><topic>Trachea - chemistry</topic><topic>Trachea - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Caldwell, Ray A</creatorcontrib><creatorcontrib>Grubb, Barbara R</creatorcontrib><creatorcontrib>Tarran, Robert</creatorcontrib><creatorcontrib>Boucher, Richard C</creatorcontrib><creatorcontrib>Knowles, Michael R</creatorcontrib><creatorcontrib>Barker, Pierre M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of general physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Caldwell, Ray A</au><au>Grubb, Barbara R</au><au>Tarran, Robert</au><au>Boucher, Richard C</au><au>Knowles, Michael R</au><au>Barker, Pierre M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vivo airway surface liquid Cl- analysis with solid-state electrodes</atitle><jtitle>The Journal of general physiology</jtitle><addtitle>J Gen Physiol</addtitle><date>2002-01</date><risdate>2002</risdate><volume>119</volume><issue>1</issue><spage>3</spage><epage>14</epage><pages>3-14</pages><issn>0022-1295</issn><eissn>1540-7748</eissn><abstract>The pathogenesis of cystic fibrosis (CF) airways disease remains controversial. Hypotheses that link mutations in CFTR and defects in ion transport to CF lung disease predict that alterations in airway surface liquid (ASL) isotonic volume, or ion composition, are critically important. ASL [Cl-] is pivotal in discriminating between these hypotheses, but there is no consensus on this value given the difficulty in measuring [Cl-] in the "thin" ASL (approximately 30 microm) in vivo. Consequently, a miniaturized solid-state electrode with a shallow depth of immersion was constructed to measure ASL [Cl-] in vivo. In initial experiments, the electrode measured [Cl-] in physiologic salt solutions, small volume (7.6 microl) test solutions, and in in vitro cell culture models, with &gt; or =93% accuracy. Based on discrepancies in reported values and/or absence of data, ASL Cl- measurements were made in the following airway regions and species. First, ASL [Cl-] was measured in normal human nasal cavity and averaged 117.3 +/- 11.2 mM (n = 6). Second, ASL [Cl-] measured in large airway (tracheobronchial) regions were as follows: rabbit trachea and bronchus = 114.3 +/- 1.8 mM; (n = 6) and 126.9 +/- 1.7 mM; (n = 3), respectively; mouse trachea = 112.8 +/- 4.2 mM (n = 13); and monkey bronchus = 112.3 +/- 10.9 mM (n = 3). Third, Cl- measurements were made in small (1-2 mm) diameter airways of the rabbit (108.3 +/- 7.1 mM, n = 5) and monkey (128.5 +/- 6.8 mM, n = 3). The measured [Cl-], in excess of 100 mM throughout all airway regions tested in multiple species, is consistent with the isotonic volume hypothesis to describe ASL physiology.</abstract><cop>United States</cop><pub>The Rockefeller University Press</pub><pmid>11773234</pmid><doi>10.1085/jgp.119.1.3</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0022-1295
ispartof The Journal of general physiology, 2002-01, Vol.119 (1), p.3-14
issn 0022-1295
1540-7748
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2233861
source MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection
subjects Adult
Animals
Bronchi - chemistry
Bronchi - physiology
Chlorides - analysis
Chlorides - standards
Female
Humans
Ion-Selective Electrodes - standards
Macaca mulatta
Male
Mice
Mice, Inbred C57BL
Middle Aged
Nasal Cavity - chemistry
Nasal Cavity - physiology
Original
Rabbits
Solutions
Trachea - chemistry
Trachea - physiology
title In vivo airway surface liquid Cl- analysis with solid-state electrodes
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-05T17%3A56%3A38IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=In%20vivo%20airway%20surface%20liquid%20Cl-%20analysis%20with%20solid-state%20electrodes&rft.jtitle=The%20Journal%20of%20general%20physiology&rft.au=Caldwell,%20Ray%20A&rft.date=2002-01&rft.volume=119&rft.issue=1&rft.spage=3&rft.epage=14&rft.pages=3-14&rft.issn=0022-1295&rft.eissn=1540-7748&rft_id=info:doi/10.1085/jgp.119.1.3&rft_dat=%3Cproquest_pubme%3E71356155%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=71356155&rft_id=info:pmid/11773234&rfr_iscdi=true