Helicobacter Genotyping and Detection in Peroperative Lavage Fluid in Patients with Perforated Peptic Ulcer
Introduction and Objectives Certain Helicobacter pylori genotypes are associated with peptic ulcer disease; however, little is known about associations between the H. pylori genotype and perforated peptic ulcer (PPU). The primary aim of this study was to evaluate which genotypes are present in patie...
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| Veröffentlicht in: | Journal of gastrointestinal surgery 2008-03, Vol.12 (3), p.555-560 |
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| creator | Komen, N. A. P. Bertleff, M. J. O. E. van Doorn, L. J. Lange, J. F. de Graaf, P. W. |
| description | Introduction and Objectives
Certain
Helicobacter pylori
genotypes are associated with peptic ulcer disease; however, little is known about associations between the
H. pylori
genotype and perforated peptic ulcer (PPU). The primary aim of this study was to evaluate which genotypes are present in patients with PPU and which genotype is dominant in this population. The secondary aim was to study the possibility of determining the
H. pylori
status in a way other than by biopsy.
Materials and Methods
Serum samples, gastric tissue biopsies, lavage fluid, and fluid from the nasogastric tube were collected from patients operated upon for PPU. By means of PCR, DEIA, and LIPA the presence of the “cytotoxin associated gene” (
cagA
) and the genotype of the “vacuolating cytotoxin gene” were determined.
Results
Fluid from the nasogastric tube was obtained from 25 patients, lavage fluid from 26 patients, serum samples from 20 patients and biopsies from 18 patients. Several genotypes were found, of which the
vacA
s1
cagA
positive strains were predominant. Additionally, a correlation was found between the
H. pylori
presence in biopsy and its presence in lavage fluid (
p
= 0.015), rendering the latter as an alternative for biopsy. Sensitivity and specificity of lavage fluid analysis were 100% and 67%, respectively.
Conclusion
This study shows the
vacA
s1
cagA
positive strain is predominant in a PPU population. The correlation found between the
H. pylori
presence in biopsy and its presence in lavage fluid suggests that analysis of the lavage fluid is sufficient to determine the
H. pylori
presence. Risks associated with biopsy taking may be avoided. |
| doi_str_mv | 10.1007/s11605-007-0303-z |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2231408</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2789663211</sourcerecordid><originalsourceid>FETCH-LOGICAL-c468t-58285f39e9f856fd18f25be9663e22d759724ac9d2ce412d728aa381b08da9753</originalsourceid><addsrcrecordid>eNp1kV1rFDEUhgdR-mV_gDcyIHg3mpOZfN0IUm0rLNQLC96FbObMNnU2GZPMSvvrm3UXrUKv8nLOc96cw1tVr4C8A0LE-wTACWuKbEhL2ub-WXUEUrRNxyl_XjRR0FDGvh9WxyndEgKCgDyoDkEowhWRR9WPSxydDUtjM8b6An3Id5Pzq9r4vv6EGW12wdfO118xhgmjyW6D9cJszArr83F2_e9mKaPPqf7l8s0WHUIhsS9yys7W16PF-LJ6MZgx4en-Pamuzz9_O7tsFlcXX84-LhrbcZkbJqlkQ6tQDZLxoQc5ULZExXmLlPaCKUE7Y1VPLXZQClQa00pYEtkbJVh7Un3Y-U7zco29LYtFM-opurWJdzoYp__teHejV2GjKW2hI7IYvN0bxPBzxpT12iWL42g8hjlpQagAqdoCvvkPvA1z9OU4DQDFTnAQhYIdZWNIKeLwZxUgehuk3gWpt3IbpL4vM68f3_B3Yp9cAegOSKXlVxgfff2k6wPXCqqu</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1112237617</pqid></control><display><type>article</type><title>Helicobacter Genotyping and Detection in Peroperative Lavage Fluid in Patients with Perforated Peptic Ulcer</title><source>MEDLINE</source><source>SpringerNature Journals</source><creator>Komen, N. A. P. ; Bertleff, M. J. O. E. ; van Doorn, L. J. ; Lange, J. F. ; de Graaf, P. W.</creator><creatorcontrib>Komen, N. A. P. ; Bertleff, M. J. O. E. ; van Doorn, L. J. ; Lange, J. F. ; de Graaf, P. W.</creatorcontrib><description>Introduction and Objectives
Certain
Helicobacter pylori
genotypes are associated with peptic ulcer disease; however, little is known about associations between the
H. pylori
genotype and perforated peptic ulcer (PPU). The primary aim of this study was to evaluate which genotypes are present in patients with PPU and which genotype is dominant in this population. The secondary aim was to study the possibility of determining the
H. pylori
status in a way other than by biopsy.
Materials and Methods
Serum samples, gastric tissue biopsies, lavage fluid, and fluid from the nasogastric tube were collected from patients operated upon for PPU. By means of PCR, DEIA, and LIPA the presence of the “cytotoxin associated gene” (
cagA
) and the genotype of the “vacuolating cytotoxin gene” were determined.
Results
Fluid from the nasogastric tube was obtained from 25 patients, lavage fluid from 26 patients, serum samples from 20 patients and biopsies from 18 patients. Several genotypes were found, of which the
vacA
s1
cagA
positive strains were predominant. Additionally, a correlation was found between the
H. pylori
presence in biopsy and its presence in lavage fluid (
p
= 0.015), rendering the latter as an alternative for biopsy. Sensitivity and specificity of lavage fluid analysis were 100% and 67%, respectively.
Conclusion
This study shows the
vacA
s1
cagA
positive strain is predominant in a PPU population. The correlation found between the
H. pylori
presence in biopsy and its presence in lavage fluid suggests that analysis of the lavage fluid is sufficient to determine the
H. pylori
presence. Risks associated with biopsy taking may be avoided.</description><identifier>ISSN: 1091-255X</identifier><identifier>EISSN: 1873-4626</identifier><identifier>DOI: 10.1007/s11605-007-0303-z</identifier><identifier>PMID: 17906908</identifier><language>eng</language><publisher>New York: Springer-Verlag</publisher><subject>Adult ; Aged ; Aged, 80 and over ; Antigens, Bacterial - isolation & purification ; Bacterial Proteins - isolation & purification ; Bronchoalveolar Lavage Fluid - microbiology ; Female ; Fluids ; Gastroenterology ; Genotype ; Genotype & phenotype ; Helicobacter Infections - epidemiology ; Helicobacter pylori - genetics ; Humans ; Infections ; Male ; Medicine ; Medicine & Public Health ; Middle Aged ; Peptic Ulcer Perforation - microbiology ; Surgery ; Ulcers</subject><ispartof>Journal of gastrointestinal surgery, 2008-03, Vol.12 (3), p.555-560</ispartof><rights>The Society for Surgery of the Alimentary Tract 2007</rights><rights>The Society for Surgery of the Alimentary Tract 2008</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c468t-58285f39e9f856fd18f25be9663e22d759724ac9d2ce412d728aa381b08da9753</citedby><cites>FETCH-LOGICAL-c468t-58285f39e9f856fd18f25be9663e22d759724ac9d2ce412d728aa381b08da9753</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11605-007-0303-z$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11605-007-0303-z$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>230,314,780,784,885,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17906908$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Komen, N. A. P.</creatorcontrib><creatorcontrib>Bertleff, M. J. O. E.</creatorcontrib><creatorcontrib>van Doorn, L. J.</creatorcontrib><creatorcontrib>Lange, J. F.</creatorcontrib><creatorcontrib>de Graaf, P. W.</creatorcontrib><title>Helicobacter Genotyping and Detection in Peroperative Lavage Fluid in Patients with Perforated Peptic Ulcer</title><title>Journal of gastrointestinal surgery</title><addtitle>J Gastrointest Surg</addtitle><addtitle>J Gastrointest Surg</addtitle><description>Introduction and Objectives
Certain
Helicobacter pylori
genotypes are associated with peptic ulcer disease; however, little is known about associations between the
H. pylori
genotype and perforated peptic ulcer (PPU). The primary aim of this study was to evaluate which genotypes are present in patients with PPU and which genotype is dominant in this population. The secondary aim was to study the possibility of determining the
H. pylori
status in a way other than by biopsy.
Materials and Methods
Serum samples, gastric tissue biopsies, lavage fluid, and fluid from the nasogastric tube were collected from patients operated upon for PPU. By means of PCR, DEIA, and LIPA the presence of the “cytotoxin associated gene” (
cagA
) and the genotype of the “vacuolating cytotoxin gene” were determined.
Results
Fluid from the nasogastric tube was obtained from 25 patients, lavage fluid from 26 patients, serum samples from 20 patients and biopsies from 18 patients. Several genotypes were found, of which the
vacA
s1
cagA
positive strains were predominant. Additionally, a correlation was found between the
H. pylori
presence in biopsy and its presence in lavage fluid (
p
= 0.015), rendering the latter as an alternative for biopsy. Sensitivity and specificity of lavage fluid analysis were 100% and 67%, respectively.
Conclusion
This study shows the
vacA
s1
cagA
positive strain is predominant in a PPU population. The correlation found between the
H. pylori
presence in biopsy and its presence in lavage fluid suggests that analysis of the lavage fluid is sufficient to determine the
H. pylori
presence. Risks associated with biopsy taking may be avoided.</description><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Antigens, Bacterial - isolation & purification</subject><subject>Bacterial Proteins - isolation & purification</subject><subject>Bronchoalveolar Lavage Fluid - microbiology</subject><subject>Female</subject><subject>Fluids</subject><subject>Gastroenterology</subject><subject>Genotype</subject><subject>Genotype & phenotype</subject><subject>Helicobacter Infections - epidemiology</subject><subject>Helicobacter pylori - genetics</subject><subject>Humans</subject><subject>Infections</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Middle Aged</subject><subject>Peptic Ulcer Perforation - microbiology</subject><subject>Surgery</subject><subject>Ulcers</subject><issn>1091-255X</issn><issn>1873-4626</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNp1kV1rFDEUhgdR-mV_gDcyIHg3mpOZfN0IUm0rLNQLC96FbObMNnU2GZPMSvvrm3UXrUKv8nLOc96cw1tVr4C8A0LE-wTACWuKbEhL2ub-WXUEUrRNxyl_XjRR0FDGvh9WxyndEgKCgDyoDkEowhWRR9WPSxydDUtjM8b6An3Id5Pzq9r4vv6EGW12wdfO118xhgmjyW6D9cJszArr83F2_e9mKaPPqf7l8s0WHUIhsS9yys7W16PF-LJ6MZgx4en-Pamuzz9_O7tsFlcXX84-LhrbcZkbJqlkQ6tQDZLxoQc5ULZExXmLlPaCKUE7Y1VPLXZQClQa00pYEtkbJVh7Un3Y-U7zco29LYtFM-opurWJdzoYp__teHejV2GjKW2hI7IYvN0bxPBzxpT12iWL42g8hjlpQagAqdoCvvkPvA1z9OU4DQDFTnAQhYIdZWNIKeLwZxUgehuk3gWpt3IbpL4vM68f3_B3Yp9cAegOSKXlVxgfff2k6wPXCqqu</recordid><startdate>20080301</startdate><enddate>20080301</enddate><creator>Komen, N. A. P.</creator><creator>Bertleff, M. J. O. E.</creator><creator>van Doorn, L. J.</creator><creator>Lange, J. F.</creator><creator>de Graaf, P. 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A. P.</creatorcontrib><creatorcontrib>Bertleff, M. J. O. E.</creatorcontrib><creatorcontrib>van Doorn, L. J.</creatorcontrib><creatorcontrib>Lange, J. F.</creatorcontrib><creatorcontrib>de Graaf, P. 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A. P.</au><au>Bertleff, M. J. O. E.</au><au>van Doorn, L. J.</au><au>Lange, J. F.</au><au>de Graaf, P. W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Helicobacter Genotyping and Detection in Peroperative Lavage Fluid in Patients with Perforated Peptic Ulcer</atitle><jtitle>Journal of gastrointestinal surgery</jtitle><stitle>J Gastrointest Surg</stitle><addtitle>J Gastrointest Surg</addtitle><date>2008-03-01</date><risdate>2008</risdate><volume>12</volume><issue>3</issue><spage>555</spage><epage>560</epage><pages>555-560</pages><issn>1091-255X</issn><eissn>1873-4626</eissn><abstract>Introduction and Objectives
Certain
Helicobacter pylori
genotypes are associated with peptic ulcer disease; however, little is known about associations between the
H. pylori
genotype and perforated peptic ulcer (PPU). The primary aim of this study was to evaluate which genotypes are present in patients with PPU and which genotype is dominant in this population. The secondary aim was to study the possibility of determining the
H. pylori
status in a way other than by biopsy.
Materials and Methods
Serum samples, gastric tissue biopsies, lavage fluid, and fluid from the nasogastric tube were collected from patients operated upon for PPU. By means of PCR, DEIA, and LIPA the presence of the “cytotoxin associated gene” (
cagA
) and the genotype of the “vacuolating cytotoxin gene” were determined.
Results
Fluid from the nasogastric tube was obtained from 25 patients, lavage fluid from 26 patients, serum samples from 20 patients and biopsies from 18 patients. Several genotypes were found, of which the
vacA
s1
cagA
positive strains were predominant. Additionally, a correlation was found between the
H. pylori
presence in biopsy and its presence in lavage fluid (
p
= 0.015), rendering the latter as an alternative for biopsy. Sensitivity and specificity of lavage fluid analysis were 100% and 67%, respectively.
Conclusion
This study shows the
vacA
s1
cagA
positive strain is predominant in a PPU population. The correlation found between the
H. pylori
presence in biopsy and its presence in lavage fluid suggests that analysis of the lavage fluid is sufficient to determine the
H. pylori
presence. Risks associated with biopsy taking may be avoided.</abstract><cop>New York</cop><pub>Springer-Verlag</pub><pmid>17906908</pmid><doi>10.1007/s11605-007-0303-z</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of gastrointestinal surgery, 2008-03, Vol.12 (3), p.555-560 |
| issn | 1091-255X 1873-4626 |
| language | eng |
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| source | MEDLINE; SpringerNature Journals |
| subjects | Adult Aged Aged, 80 and over Antigens, Bacterial - isolation & purification Bacterial Proteins - isolation & purification Bronchoalveolar Lavage Fluid - microbiology Female Fluids Gastroenterology Genotype Genotype & phenotype Helicobacter Infections - epidemiology Helicobacter pylori - genetics Humans Infections Male Medicine Medicine & Public Health Middle Aged Peptic Ulcer Perforation - microbiology Surgery Ulcers |
| title | Helicobacter Genotyping and Detection in Peroperative Lavage Fluid in Patients with Perforated Peptic Ulcer |
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