A Cytological Analysis of the Antimetabolite Activity of 5-Hydroxyuracil in Vicia faba Roots
The effects of 5-hydroxyuracil (5-HU) (isobarbituric acid) upon cell elongation, mitosis, and DNA synthesis were studied in Vicia faba roots. 5-HU had no consistent effect upon root elongation. It blocked DNA synthesis (analyzed by photometric measurements of Feulgen dye in nuclei) during the first...
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| Veröffentlicht in: | Journal of biophysical and biochemical cytology 1958-11, Vol.4 (6), p.793-806 |
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| creator | Schreiber, Richard W. Duncan, Robert E. |
| description | The effects of 5-hydroxyuracil (5-HU) (isobarbituric acid) upon cell elongation, mitosis, and DNA synthesis were studied in Vicia faba roots. 5-HU had no consistent effect upon root elongation. It blocked DNA synthesis (analyzed by photometric measurements of Feulgen dye in nuclei) during the first 6 hours of treatment; the block spontaneously disappeared by the 12th hour of treatment. Uracil and thymine had no effect upon this block of synthesis. Both thymidine and uridine reversed the block in 6 and 9 hours respectively. In all cases blockage of DNA synthesis was followed by inhibition of mitosis (determined by changes in the percentage of cells in mitosis) and resumption of DNA synthesis was followed by resumption of mitosis. Inhibition indices calculated from the mitotic data indicated a competitive relationship between 5-HU and thymidine and 5-HU and uridine. 5-HU is considered to block DNA synthesis by competing with thymidine for sites on enzymes involved in the synthesis. It is suggested that uridine reverses the block in synthesis by undergoing a conversion to thymidine. |
| doi_str_mv | 10.1083/jcb.4.6.793 |
| format | Article |
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It blocked DNA synthesis (analyzed by photometric measurements of Feulgen dye in nuclei) during the first 6 hours of treatment; the block spontaneously disappeared by the 12th hour of treatment. Uracil and thymine had no effect upon this block of synthesis. Both thymidine and uridine reversed the block in 6 and 9 hours respectively. In all cases blockage of DNA synthesis was followed by inhibition of mitosis (determined by changes in the percentage of cells in mitosis) and resumption of DNA synthesis was followed by resumption of mitosis. Inhibition indices calculated from the mitotic data indicated a competitive relationship between 5-HU and thymidine and 5-HU and uridine. 5-HU is considered to block DNA synthesis by competing with thymidine for sites on enzymes involved in the synthesis. It is suggested that uridine reverses the block in synthesis by undergoing a conversion to thymidine.</description><identifier>ISSN: 0095-9901</identifier><identifier>ISSN: 0021-9525</identifier><identifier>EISSN: 1540-8140</identifier><identifier>DOI: 10.1083/jcb.4.6.793</identifier><identifier>PMID: 13610946</identifier><language>eng</language><publisher>United States: Rockefeller Institute</publisher><subject>Antimetabolites ; Cell growth ; Cell Nucleus ; DNA ; DNA - metabolism ; DNA Replication ; Enzymes ; Fabaceae ; Interphase ; Mitosis ; Nucleosides ; Old Medline ; Plant growth ; Plant Roots ; Root growth ; Teeth ; Thymidine ; Thymine ; Uracil - analogs & derivatives ; Uridine ; Vicia faba</subject><ispartof>Journal of biophysical and biochemical cytology, 1958-11, Vol.4 (6), p.793-806</ispartof><rights>Copyright 1958 The Rockefeller Institute</rights><rights>Copyright © Copyright, 1958, by The Rockefeller Institute</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c400t-11e0b07cd5737316ab56b5a3e696085deafa022458303743fa9d16be004c27c43</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2224534/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2224534/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27923,27924,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/13610946$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Schreiber, Richard W.</creatorcontrib><creatorcontrib>Duncan, Robert E.</creatorcontrib><title>A Cytological Analysis of the Antimetabolite Activity of 5-Hydroxyuracil in Vicia faba Roots</title><title>Journal of biophysical and biochemical cytology</title><addtitle>J Biophys Biochem Cytol</addtitle><description>The effects of 5-hydroxyuracil (5-HU) (isobarbituric acid) upon cell elongation, mitosis, and DNA synthesis were studied in Vicia faba roots. 5-HU had no consistent effect upon root elongation. It blocked DNA synthesis (analyzed by photometric measurements of Feulgen dye in nuclei) during the first 6 hours of treatment; the block spontaneously disappeared by the 12th hour of treatment. Uracil and thymine had no effect upon this block of synthesis. Both thymidine and uridine reversed the block in 6 and 9 hours respectively. In all cases blockage of DNA synthesis was followed by inhibition of mitosis (determined by changes in the percentage of cells in mitosis) and resumption of DNA synthesis was followed by resumption of mitosis. Inhibition indices calculated from the mitotic data indicated a competitive relationship between 5-HU and thymidine and 5-HU and uridine. 5-HU is considered to block DNA synthesis by competing with thymidine for sites on enzymes involved in the synthesis. It is suggested that uridine reverses the block in synthesis by undergoing a conversion to thymidine.</description><subject>Antimetabolites</subject><subject>Cell growth</subject><subject>Cell Nucleus</subject><subject>DNA</subject><subject>DNA - metabolism</subject><subject>DNA Replication</subject><subject>Enzymes</subject><subject>Fabaceae</subject><subject>Interphase</subject><subject>Mitosis</subject><subject>Nucleosides</subject><subject>Old Medline</subject><subject>Plant growth</subject><subject>Plant Roots</subject><subject>Root growth</subject><subject>Teeth</subject><subject>Thymidine</subject><subject>Thymine</subject><subject>Uracil - analogs & derivatives</subject><subject>Uridine</subject><subject>Vicia faba</subject><issn>0095-9901</issn><issn>0021-9525</issn><issn>1540-8140</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1958</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkc1r20AQxZfS0DhpT72WolMuQc7sp6RLwZh8FAKB0PZUWEarVbJmrU1316b67yNjk4_T8Hg_3gzzCPlKYU6h5hcr087FXM2rhn8gMyoFlDUV8JHMABpZNg3QY3KS0gqAVaKWn8gx5YpCI9SM_F0UyzEHHx6cQV8sBvRjcqkIfZEf7aSzW9uMbfAuT9Jkt3V53NmyvBm7GP6Pm4jG-cINxR9nHBY9tljch5DTZ3LUo0_2y2Gekt9Xl7-WN-Xt3fXP5eK2NAIgl5RaaKEynax4xanCVqpWIreqUVDLzmKPwJiQNQdeCd5j01HVWgBhWGUEPyU_9rlPm3ZtO2OHHNHrp-jWGEcd0On3zuAe9UPYarZL5buAs0NADP82NmW9dslY73GwYZN0zRivGDQTeL4HTQwpRdu_LKGgd23oqQ0ttNJTGxP9_e1dr-zh_RPwbQ-sUg7x1VfAmaL8GfhAj6w</recordid><startdate>19581125</startdate><enddate>19581125</enddate><creator>Schreiber, Richard W.</creator><creator>Duncan, Robert E.</creator><general>Rockefeller Institute</general><general>The Rockefeller University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19581125</creationdate><title>A Cytological Analysis of the Antimetabolite Activity of 5-Hydroxyuracil in Vicia faba Roots</title><author>Schreiber, Richard W. ; Duncan, Robert E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c400t-11e0b07cd5737316ab56b5a3e696085deafa022458303743fa9d16be004c27c43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1958</creationdate><topic>Antimetabolites</topic><topic>Cell growth</topic><topic>Cell Nucleus</topic><topic>DNA</topic><topic>DNA - metabolism</topic><topic>DNA Replication</topic><topic>Enzymes</topic><topic>Fabaceae</topic><topic>Interphase</topic><topic>Mitosis</topic><topic>Nucleosides</topic><topic>Old Medline</topic><topic>Plant growth</topic><topic>Plant Roots</topic><topic>Root growth</topic><topic>Teeth</topic><topic>Thymidine</topic><topic>Thymine</topic><topic>Uracil - analogs & derivatives</topic><topic>Uridine</topic><topic>Vicia faba</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schreiber, Richard W.</creatorcontrib><creatorcontrib>Duncan, Robert E.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of biophysical and biochemical cytology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schreiber, Richard W.</au><au>Duncan, Robert E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Cytological Analysis of the Antimetabolite Activity of 5-Hydroxyuracil in Vicia faba Roots</atitle><jtitle>Journal of biophysical and biochemical cytology</jtitle><addtitle>J Biophys Biochem Cytol</addtitle><date>1958-11-25</date><risdate>1958</risdate><volume>4</volume><issue>6</issue><spage>793</spage><epage>806</epage><pages>793-806</pages><issn>0095-9901</issn><issn>0021-9525</issn><eissn>1540-8140</eissn><abstract>The effects of 5-hydroxyuracil (5-HU) (isobarbituric acid) upon cell elongation, mitosis, and DNA synthesis were studied in Vicia faba roots. 5-HU had no consistent effect upon root elongation. It blocked DNA synthesis (analyzed by photometric measurements of Feulgen dye in nuclei) during the first 6 hours of treatment; the block spontaneously disappeared by the 12th hour of treatment. Uracil and thymine had no effect upon this block of synthesis. Both thymidine and uridine reversed the block in 6 and 9 hours respectively. In all cases blockage of DNA synthesis was followed by inhibition of mitosis (determined by changes in the percentage of cells in mitosis) and resumption of DNA synthesis was followed by resumption of mitosis. Inhibition indices calculated from the mitotic data indicated a competitive relationship between 5-HU and thymidine and 5-HU and uridine. 5-HU is considered to block DNA synthesis by competing with thymidine for sites on enzymes involved in the synthesis. It is suggested that uridine reverses the block in synthesis by undergoing a conversion to thymidine.</abstract><cop>United States</cop><pub>Rockefeller Institute</pub><pmid>13610946</pmid><doi>10.1083/jcb.4.6.793</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of biophysical and biochemical cytology, 1958-11, Vol.4 (6), p.793-806 |
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| source | MEDLINE; PubMed Central Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Antimetabolites Cell growth Cell Nucleus DNA DNA - metabolism DNA Replication Enzymes Fabaceae Interphase Mitosis Nucleosides Old Medline Plant growth Plant Roots Root growth Teeth Thymidine Thymine Uracil - analogs & derivatives Uridine Vicia faba |
| title | A Cytological Analysis of the Antimetabolite Activity of 5-Hydroxyuracil in Vicia faba Roots |
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