TIMP-3 mRNA expression is regionally increased in moderately and poorly differentiated colorectal adenocarcinoma
In this study, we report on the distribution of tissue inhibitor of matrix metalloproteinase-3 (TIMP-3) mRNA expression in human normal colorectal mucosa, adenomas and adenocarcinomas. Northern blot analysis showed five TIMP-3 mRNA transcripts to be present in normal mucosal epithelium and in modera...
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| Veröffentlicht in: | British journal of cancer 1997-06, Vol.75 (11), p.1678-1683 |
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| creator | Powe, DG Brough, JL Carter, GI Bailey, EM Stetler-Stevenson, WG Turner, DR Hewitt, RE |
| description | In this study, we report on the distribution of tissue inhibitor of matrix metalloproteinase-3 (TIMP-3) mRNA expression in human normal colorectal mucosa, adenomas and adenocarcinomas. Northern blot analysis showed five TIMP-3 mRNA transcripts to be present in normal mucosal epithelium and in moderately and poorly differentiated carcinoma. Adenomas and well-differentiated carcinomas were not examined in this part of the investigation. In situ hybridization studies showed no detectable TIMP-3 mRNA in normal and adenomatous tissue. In contrast, TIMP-3 mRNA is localized to stromal fibroblast-like cells in colorectal carcinomas, with an increased incidence in moderately and poorly differentiated groups compared with well-differentiated carcinomas. Expression in both the moderately and the poorly differentiated tumour groups was strongest at the tumour invasive edge; none of the poorly differentiated carcinomas showed mRNA expression in regions ahead of the invasive edge, compared with 3 of 12 of the moderate group. To our knowledge, this is the first detailed report on the regional localization of TIMP-3 mRNA in colorectal tumours. We suggest that the lack of TIMP-3 mRNA expression in host stromal tissues ahead of poorly differentiated carcinomas may contribute to their increased invasiveness. |
| doi_str_mv | 10.1038/bjc.1997.285 |
| format | Article |
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Northern blot analysis showed five TIMP-3 mRNA transcripts to be present in normal mucosal epithelium and in moderately and poorly differentiated carcinoma. Adenomas and well-differentiated carcinomas were not examined in this part of the investigation. In situ hybridization studies showed no detectable TIMP-3 mRNA in normal and adenomatous tissue. In contrast, TIMP-3 mRNA is localized to stromal fibroblast-like cells in colorectal carcinomas, with an increased incidence in moderately and poorly differentiated groups compared with well-differentiated carcinomas. Expression in both the moderately and the poorly differentiated tumour groups was strongest at the tumour invasive edge; none of the poorly differentiated carcinomas showed mRNA expression in regions ahead of the invasive edge, compared with 3 of 12 of the moderate group. To our knowledge, this is the first detailed report on the regional localization of TIMP-3 mRNA in colorectal tumours. We suggest that the lack of TIMP-3 mRNA expression in host stromal tissues ahead of poorly differentiated carcinomas may contribute to their increased invasiveness.</description><identifier>ISSN: 0007-0920</identifier><identifier>EISSN: 1532-1827</identifier><identifier>DOI: 10.1038/bjc.1997.285</identifier><identifier>PMID: 9184186</identifier><identifier>CODEN: BJCAAI</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Adenocarcinoma - metabolism ; Biological and medical sciences ; Biomedical and Life Sciences ; Biomedicine ; Blotting, Northern ; Cancer Research ; clinical-oncology ; Colorectal Neoplasms - metabolism ; Drug Resistance ; Epidemiology ; Gastroenterology. Liver. Pancreas. Abdomen ; Humans ; In Situ Hybridization ; Medical sciences ; Molecular Medicine ; Oncology ; Protease Inhibitors - metabolism ; Proteins - genetics ; RNA, Messenger - analysis ; Stomach. Duodenum. Small intestine. Colon. Rectum. Anus ; Tissue Inhibitor of Metalloproteinase-3 ; Tumors</subject><ispartof>British journal of cancer, 1997-06, Vol.75 (11), p.1678-1683</ispartof><rights>Cancer Research Campaign 1997</rights><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c537t-24e16d579b8e8076f8d79144e5f5a80955bb1872dd700cda51e44adb9cf4a4253</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2223522/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2223522/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,2727,27924,27925,41488,42557,51319,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2677202$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9184186$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Powe, DG</creatorcontrib><creatorcontrib>Brough, JL</creatorcontrib><creatorcontrib>Carter, GI</creatorcontrib><creatorcontrib>Bailey, EM</creatorcontrib><creatorcontrib>Stetler-Stevenson, WG</creatorcontrib><creatorcontrib>Turner, DR</creatorcontrib><creatorcontrib>Hewitt, RE</creatorcontrib><title>TIMP-3 mRNA expression is regionally increased in moderately and poorly differentiated colorectal adenocarcinoma</title><title>British journal of cancer</title><addtitle>Br J Cancer</addtitle><addtitle>Br J Cancer</addtitle><description>In this study, we report on the distribution of tissue inhibitor of matrix metalloproteinase-3 (TIMP-3) mRNA expression in human normal colorectal mucosa, adenomas and adenocarcinomas. Northern blot analysis showed five TIMP-3 mRNA transcripts to be present in normal mucosal epithelium and in moderately and poorly differentiated carcinoma. Adenomas and well-differentiated carcinomas were not examined in this part of the investigation. In situ hybridization studies showed no detectable TIMP-3 mRNA in normal and adenomatous tissue. In contrast, TIMP-3 mRNA is localized to stromal fibroblast-like cells in colorectal carcinomas, with an increased incidence in moderately and poorly differentiated groups compared with well-differentiated carcinomas. Expression in both the moderately and the poorly differentiated tumour groups was strongest at the tumour invasive edge; none of the poorly differentiated carcinomas showed mRNA expression in regions ahead of the invasive edge, compared with 3 of 12 of the moderate group. To our knowledge, this is the first detailed report on the regional localization of TIMP-3 mRNA in colorectal tumours. We suggest that the lack of TIMP-3 mRNA expression in host stromal tissues ahead of poorly differentiated carcinomas may contribute to their increased invasiveness.</description><subject>Adenocarcinoma - metabolism</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Blotting, Northern</subject><subject>Cancer Research</subject><subject>clinical-oncology</subject><subject>Colorectal Neoplasms - metabolism</subject><subject>Drug Resistance</subject><subject>Epidemiology</subject><subject>Gastroenterology. Liver. Pancreas. Abdomen</subject><subject>Humans</subject><subject>In Situ Hybridization</subject><subject>Medical sciences</subject><subject>Molecular Medicine</subject><subject>Oncology</subject><subject>Protease Inhibitors - metabolism</subject><subject>Proteins - genetics</subject><subject>RNA, Messenger - analysis</subject><subject>Stomach. Duodenum. Small intestine. Colon. Rectum. Anus</subject><subject>Tissue Inhibitor of Metalloproteinase-3</subject><subject>Tumors</subject><issn>0007-0920</issn><issn>1532-1827</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kc2LFDEQxYMo6-zozauQg3iyxyTdmaQvwrLourB-IOs5VCfVY4bupE16xP3vTTPDoAcPIS95P14VPEJecLbhrNZvu73d8LZVG6HlI7LishYV10I9JivGmKpYK9hTcpnzvjxbptUFuWi5brjersh0f_vpa1XT8dvnK4q_p4Q5-xiozzThrigYhgfqg00IGV1RdIwOE8xY_iE4OsWYinS-7zFhmH2xHLVxiAntDAMFhyFaSNaHOMIz8qSHIePz070m3z-8v7_-WN19ubm9vrqrrKzVXIkG-dZJ1XYaNVPbXjvV8qZB2UvQrJWy67hWwjnFmHUgOTYNuK61fQONkPWavDvmToduRGfLZgkGMyU_QnowEbz51wn-h9nFX0YIUcty1uT1KSDFnwfMsxl9tjgMEDAeslEta5ZBBXxzBG2KOSfsz0M4M0tDpjRkloZMaajgL_9e7AyfKin-q5MP2cLQJwjW5zMmtkoJtqxXHbFcnLDDZPbxkEpd-X9j6ZEPMB8SnvMKtDAL8geHurWf</recordid><startdate>19970601</startdate><enddate>19970601</enddate><creator>Powe, DG</creator><creator>Brough, JL</creator><creator>Carter, GI</creator><creator>Bailey, EM</creator><creator>Stetler-Stevenson, WG</creator><creator>Turner, DR</creator><creator>Hewitt, RE</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19970601</creationdate><title>TIMP-3 mRNA expression is regionally increased in moderately and poorly differentiated colorectal adenocarcinoma</title><author>Powe, DG ; Brough, JL ; Carter, GI ; Bailey, EM ; Stetler-Stevenson, WG ; Turner, DR ; Hewitt, RE</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c537t-24e16d579b8e8076f8d79144e5f5a80955bb1872dd700cda51e44adb9cf4a4253</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Adenocarcinoma - metabolism</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Blotting, Northern</topic><topic>Cancer Research</topic><topic>clinical-oncology</topic><topic>Colorectal Neoplasms - metabolism</topic><topic>Drug Resistance</topic><topic>Epidemiology</topic><topic>Gastroenterology. Liver. Pancreas. Abdomen</topic><topic>Humans</topic><topic>In Situ Hybridization</topic><topic>Medical sciences</topic><topic>Molecular Medicine</topic><topic>Oncology</topic><topic>Protease Inhibitors - metabolism</topic><topic>Proteins - genetics</topic><topic>RNA, Messenger - analysis</topic><topic>Stomach. Duodenum. Small intestine. Colon. Rectum. Anus</topic><topic>Tissue Inhibitor of Metalloproteinase-3</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Powe, DG</creatorcontrib><creatorcontrib>Brough, JL</creatorcontrib><creatorcontrib>Carter, GI</creatorcontrib><creatorcontrib>Bailey, EM</creatorcontrib><creatorcontrib>Stetler-Stevenson, WG</creatorcontrib><creatorcontrib>Turner, DR</creatorcontrib><creatorcontrib>Hewitt, RE</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>British journal of cancer</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Powe, DG</au><au>Brough, JL</au><au>Carter, GI</au><au>Bailey, EM</au><au>Stetler-Stevenson, WG</au><au>Turner, DR</au><au>Hewitt, RE</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>TIMP-3 mRNA expression is regionally increased in moderately and poorly differentiated colorectal adenocarcinoma</atitle><jtitle>British journal of cancer</jtitle><stitle>Br J Cancer</stitle><addtitle>Br J Cancer</addtitle><date>1997-06-01</date><risdate>1997</risdate><volume>75</volume><issue>11</issue><spage>1678</spage><epage>1683</epage><pages>1678-1683</pages><issn>0007-0920</issn><eissn>1532-1827</eissn><coden>BJCAAI</coden><abstract>In this study, we report on the distribution of tissue inhibitor of matrix metalloproteinase-3 (TIMP-3) mRNA expression in human normal colorectal mucosa, adenomas and adenocarcinomas. Northern blot analysis showed five TIMP-3 mRNA transcripts to be present in normal mucosal epithelium and in moderately and poorly differentiated carcinoma. Adenomas and well-differentiated carcinomas were not examined in this part of the investigation. In situ hybridization studies showed no detectable TIMP-3 mRNA in normal and adenomatous tissue. In contrast, TIMP-3 mRNA is localized to stromal fibroblast-like cells in colorectal carcinomas, with an increased incidence in moderately and poorly differentiated groups compared with well-differentiated carcinomas. Expression in both the moderately and the poorly differentiated tumour groups was strongest at the tumour invasive edge; none of the poorly differentiated carcinomas showed mRNA expression in regions ahead of the invasive edge, compared with 3 of 12 of the moderate group. To our knowledge, this is the first detailed report on the regional localization of TIMP-3 mRNA in colorectal tumours. 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| subjects | Adenocarcinoma - metabolism Biological and medical sciences Biomedical and Life Sciences Biomedicine Blotting, Northern Cancer Research clinical-oncology Colorectal Neoplasms - metabolism Drug Resistance Epidemiology Gastroenterology. Liver. Pancreas. Abdomen Humans In Situ Hybridization Medical sciences Molecular Medicine Oncology Protease Inhibitors - metabolism Proteins - genetics RNA, Messenger - analysis Stomach. Duodenum. Small intestine. Colon. Rectum. Anus Tissue Inhibitor of Metalloproteinase-3 Tumors |
| title | TIMP-3 mRNA expression is regionally increased in moderately and poorly differentiated colorectal adenocarcinoma |
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