A dihydropyridine-sensitive voltage-dependent calcium channel in the sarcolemmal membrane of crustacean muscle
Single-channel currents through calcium channels in muscle of a marine crustacean, the isopod Idotea baltica, were investigated in cell-attached patches. Inward barium currents were strongly voltage-dependent, and the channels were closed at the cell's resting membrane potential. The open proba...
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| Veröffentlicht in: | The Journal of general physiology 1997-03, Vol.109 (3), p.313-326 |
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| creator | Erxleben, C Rathmayer, W |
| description | Single-channel currents through calcium channels in muscle of a marine crustacean, the isopod Idotea baltica, were investigated in cell-attached patches. Inward barium currents were strongly voltage-dependent, and the channels were closed at the cell's resting membrane potential. The open probability (Po) increased e-fold for an 8.2 mV (+/- 2.4, n = 13) depolarization. Channel opening were mainly brief (< 0.3 ms) and evenly distributed throughout 100-ms pulses. Averaged, quasimacroscopic currents showed fast activation and deactivation and did not inactivate during 100-ms test pulses. Similarly, channel activity persisted at steadily depolarized holding potentials. With 200 mM Ba2+ as charge carrier, the average slope conductance from the unitary currents between +30 and +80 mV, was 20 pS (+/- 2.6, n = 12). The proportion of long openings, which were very infrequent under control conditions, was greatly increased by preincubation of the muscle fibers with the calcium channel agonist, the dihydropyridine Bay K8644 (10-100 microM). Properties of these currents resemble those through the L-type calcium channels of mammalian nerve, smooth muscle, and cardiac muscle cells. |
| doi_str_mv | 10.1085/jgp.109.3.313 |
| format | Article |
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Inward barium currents were strongly voltage-dependent, and the channels were closed at the cell's resting membrane potential. The open probability (Po) increased e-fold for an 8.2 mV (+/- 2.4, n = 13) depolarization. Channel opening were mainly brief (< 0.3 ms) and evenly distributed throughout 100-ms pulses. Averaged, quasimacroscopic currents showed fast activation and deactivation and did not inactivate during 100-ms test pulses. Similarly, channel activity persisted at steadily depolarized holding potentials. With 200 mM Ba2+ as charge carrier, the average slope conductance from the unitary currents between +30 and +80 mV, was 20 pS (+/- 2.6, n = 12). The proportion of long openings, which were very infrequent under control conditions, was greatly increased by preincubation of the muscle fibers with the calcium channel agonist, the dihydropyridine Bay K8644 (10-100 microM). Properties of these currents resemble those through the L-type calcium channels of mammalian nerve, smooth muscle, and cardiac muscle cells.</description><identifier>ISSN: 0022-1295</identifier><identifier>EISSN: 1540-7748</identifier><identifier>DOI: 10.1085/jgp.109.3.313</identifier><identifier>PMID: 9089439</identifier><identifier>CODEN: JGPLAD</identifier><language>eng</language><publisher>United States: Rockefeller University Press</publisher><subject>3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester - pharmacology ; Animals ; Barium - metabolism ; Biochemistry ; Calcium ; Calcium Channel Agonists - pharmacology ; Calcium Channel Blockers - pharmacology ; Calcium Channels - drug effects ; Calcium Channels - physiology ; Crustacea - metabolism ; Crustaceans ; Dihydropyridines - pharmacology ; Electrophysiology ; In Vitro Techniques ; Ion Channel Gating - drug effects ; Ion Channel Gating - physiology ; Marine biology ; Membrane Potentials - drug effects ; Membrane Potentials - physiology ; Membranes ; Muscle Contraction - drug effects ; Muscle Contraction - physiology ; Muscle Fibers, Skeletal - physiology ; Muscles - drug effects ; Muscles - metabolism ; Muscles - ultrastructure ; Muscular system ; Patch-Clamp Techniques ; Sarcolemma - drug effects ; Sarcolemma - metabolism</subject><ispartof>The Journal of general physiology, 1997-03, Vol.109 (3), p.313-326</ispartof><rights>Copyright Rockefeller University Press Mar 1997</rights><rights>1997</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c409t-c42833436f86b4499b1f66a19045bddabee961b843479b426687d3d34aaec86b3</citedby><cites>FETCH-LOGICAL-c409t-c42833436f86b4499b1f66a19045bddabee961b843479b426687d3d34aaec86b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9089439$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Erxleben, C</creatorcontrib><creatorcontrib>Rathmayer, W</creatorcontrib><title>A dihydropyridine-sensitive voltage-dependent calcium channel in the sarcolemmal membrane of crustacean muscle</title><title>The Journal of general physiology</title><addtitle>J Gen Physiol</addtitle><description>Single-channel currents through calcium channels in muscle of a marine crustacean, the isopod Idotea baltica, were investigated in cell-attached patches. Inward barium currents were strongly voltage-dependent, and the channels were closed at the cell's resting membrane potential. The open probability (Po) increased e-fold for an 8.2 mV (+/- 2.4, n = 13) depolarization. Channel opening were mainly brief (< 0.3 ms) and evenly distributed throughout 100-ms pulses. Averaged, quasimacroscopic currents showed fast activation and deactivation and did not inactivate during 100-ms test pulses. Similarly, channel activity persisted at steadily depolarized holding potentials. With 200 mM Ba2+ as charge carrier, the average slope conductance from the unitary currents between +30 and +80 mV, was 20 pS (+/- 2.6, n = 12). The proportion of long openings, which were very infrequent under control conditions, was greatly increased by preincubation of the muscle fibers with the calcium channel agonist, the dihydropyridine Bay K8644 (10-100 microM). Properties of these currents resemble those through the L-type calcium channels of mammalian nerve, smooth muscle, and cardiac muscle cells.</description><subject>3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester - pharmacology</subject><subject>Animals</subject><subject>Barium - metabolism</subject><subject>Biochemistry</subject><subject>Calcium</subject><subject>Calcium Channel Agonists - pharmacology</subject><subject>Calcium Channel Blockers - pharmacology</subject><subject>Calcium Channels - drug effects</subject><subject>Calcium Channels - physiology</subject><subject>Crustacea - metabolism</subject><subject>Crustaceans</subject><subject>Dihydropyridines - pharmacology</subject><subject>Electrophysiology</subject><subject>In Vitro Techniques</subject><subject>Ion Channel Gating - drug effects</subject><subject>Ion Channel Gating - physiology</subject><subject>Marine biology</subject><subject>Membrane Potentials - drug effects</subject><subject>Membrane Potentials - physiology</subject><subject>Membranes</subject><subject>Muscle Contraction - drug effects</subject><subject>Muscle Contraction - physiology</subject><subject>Muscle Fibers, Skeletal - physiology</subject><subject>Muscles - drug effects</subject><subject>Muscles - metabolism</subject><subject>Muscles - ultrastructure</subject><subject>Muscular system</subject><subject>Patch-Clamp Techniques</subject><subject>Sarcolemma - drug effects</subject><subject>Sarcolemma - metabolism</subject><issn>0022-1295</issn><issn>1540-7748</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkUlrHDEQhYVJcMZOjj4GRA659URbL7oEjEmcgCGX-CzUUvWMBi0dqXtg_r1lPJgkOpQK6qvHKx5CN5RsKRnaL4fdXBu55VtO-QXa0FaQpu_F8AZtCGGsoUy279BVKQdSX8vIJbqUZJCCyw2Kt9i6_cnmNJ-ysy5CUyAWt7gj4GPyi95BY2GGaCEu2Ghv3Bqw2esYwWMX8bIHXHQ2yUMI2uMAYcw6Ak4TNnktizagIw5rMR7eo7eT9gU-nP9r9Pj92--7H83Dr_ufd7cPjRFELrWygXPBu2noRiGkHOnUdZpKItrRWj0CyI6Og-Cil6NgXTf0llsutAZTV_g1-vqiO69jAGuq96y9mrMLOp9U0k79O4lur3bpqBijPel5Ffh8FsjpzwplUcEVA97Xy9JaVD9IInnfVvDTf-AhrTnW4xQjLaWMd6RCzQtkciolw_TqhBL1nKKqKdZGKq5qipX_-Lf9V_ocG38ChxKbMA</recordid><startdate>19970301</startdate><enddate>19970301</enddate><creator>Erxleben, C</creator><creator>Rathmayer, W</creator><general>Rockefeller University Press</general><general>The Rockefeller University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TS</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19970301</creationdate><title>A dihydropyridine-sensitive voltage-dependent calcium channel in the sarcolemmal membrane of crustacean muscle</title><author>Erxleben, C ; Rathmayer, W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c409t-c42833436f86b4499b1f66a19045bddabee961b843479b426687d3d34aaec86b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester - pharmacology</topic><topic>Animals</topic><topic>Barium - metabolism</topic><topic>Biochemistry</topic><topic>Calcium</topic><topic>Calcium Channel Agonists - pharmacology</topic><topic>Calcium Channel Blockers - pharmacology</topic><topic>Calcium Channels - drug effects</topic><topic>Calcium Channels - physiology</topic><topic>Crustacea - metabolism</topic><topic>Crustaceans</topic><topic>Dihydropyridines - pharmacology</topic><topic>Electrophysiology</topic><topic>In Vitro Techniques</topic><topic>Ion Channel Gating - drug effects</topic><topic>Ion Channel Gating - physiology</topic><topic>Marine biology</topic><topic>Membrane Potentials - drug effects</topic><topic>Membrane Potentials - physiology</topic><topic>Membranes</topic><topic>Muscle Contraction - drug effects</topic><topic>Muscle Contraction - physiology</topic><topic>Muscle Fibers, Skeletal - physiology</topic><topic>Muscles - drug effects</topic><topic>Muscles - metabolism</topic><topic>Muscles - ultrastructure</topic><topic>Muscular system</topic><topic>Patch-Clamp Techniques</topic><topic>Sarcolemma - drug effects</topic><topic>Sarcolemma - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Erxleben, C</creatorcontrib><creatorcontrib>Rathmayer, W</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Physical Education Index</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of general physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Erxleben, C</au><au>Rathmayer, W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A dihydropyridine-sensitive voltage-dependent calcium channel in the sarcolemmal membrane of crustacean muscle</atitle><jtitle>The Journal of general physiology</jtitle><addtitle>J Gen Physiol</addtitle><date>1997-03-01</date><risdate>1997</risdate><volume>109</volume><issue>3</issue><spage>313</spage><epage>326</epage><pages>313-326</pages><issn>0022-1295</issn><eissn>1540-7748</eissn><coden>JGPLAD</coden><abstract>Single-channel currents through calcium channels in muscle of a marine crustacean, the isopod Idotea baltica, were investigated in cell-attached patches. Inward barium currents were strongly voltage-dependent, and the channels were closed at the cell's resting membrane potential. The open probability (Po) increased e-fold for an 8.2 mV (+/- 2.4, n = 13) depolarization. Channel opening were mainly brief (< 0.3 ms) and evenly distributed throughout 100-ms pulses. Averaged, quasimacroscopic currents showed fast activation and deactivation and did not inactivate during 100-ms test pulses. Similarly, channel activity persisted at steadily depolarized holding potentials. With 200 mM Ba2+ as charge carrier, the average slope conductance from the unitary currents between +30 and +80 mV, was 20 pS (+/- 2.6, n = 12). The proportion of long openings, which were very infrequent under control conditions, was greatly increased by preincubation of the muscle fibers with the calcium channel agonist, the dihydropyridine Bay K8644 (10-100 microM). Properties of these currents resemble those through the L-type calcium channels of mammalian nerve, smooth muscle, and cardiac muscle cells.</abstract><cop>United States</cop><pub>Rockefeller University Press</pub><pmid>9089439</pmid><doi>10.1085/jgp.109.3.313</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester - pharmacology Animals Barium - metabolism Biochemistry Calcium Calcium Channel Agonists - pharmacology Calcium Channel Blockers - pharmacology Calcium Channels - drug effects Calcium Channels - physiology Crustacea - metabolism Crustaceans Dihydropyridines - pharmacology Electrophysiology In Vitro Techniques Ion Channel Gating - drug effects Ion Channel Gating - physiology Marine biology Membrane Potentials - drug effects Membrane Potentials - physiology Membranes Muscle Contraction - drug effects Muscle Contraction - physiology Muscle Fibers, Skeletal - physiology Muscles - drug effects Muscles - metabolism Muscles - ultrastructure Muscular system Patch-Clamp Techniques Sarcolemma - drug effects Sarcolemma - metabolism |
| title | A dihydropyridine-sensitive voltage-dependent calcium channel in the sarcolemmal membrane of crustacean muscle |
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