Distinct Activities of the Germline and Somatic Reproductive Tissues in the Regulation of Caenorhabditis elegans' Longevity
The two parts of the Caenorhabditis elegans reproductive system, the germ cells and the somatic reproductive tissues, each influence the life span of the animal. Removing the germ cells increases longevity, and this life span extension requires the somatic gonad. Here we show that the somatic gonad...
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| Veröffentlicht in: | Genetics (Austin) 2008-01, Vol.178 (1), p.513-526 |
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| creator | Yamawaki, Tracy M Arantes-Oliveira, Nuno Berman, Jennifer R Zhang, Peichuan Kenyon, Cynthia |
| description | The two parts of the Caenorhabditis elegans reproductive system, the germ cells and the somatic reproductive tissues, each influence the life span of the animal. Removing the germ cells increases longevity, and this life span extension requires the somatic gonad. Here we show that the somatic gonad and the germ cells make distinct contributions to life span determination. The life span increase produced by loss of the germ cells requires the DAF-16/FOXO transcription factor. In response to germ-cell removal, DAF-16 accumulates in nuclei. We find that the somatic gonad is not required for DAF-16 nuclear accumulation or for the increased stress resistance that is produced by germ-cell removal. The somatic gonad is required, however, for expression of specific DAF-16 target genes. DAF-16 is known to be activated by reduced insulin/IGF-1 signaling in C. elegans. In certain insulin/IGF-1-pathway mutants, the somatic gonad is not required for germ-cell removal to extend life span. Our genetic experiments suggest that these mutations reduce insulin/IGF-1 signaling below a critical threshold level. At these low levels of insulin/IGF-1 signaling, factors normally provided by the somatic gonad are no longer needed for germ-cell removal to increase the expression of DAF-16 target genes. |
| doi_str_mv | 10.1534/genetics.107.083253 |
| format | Article |
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Removing the germ cells increases longevity, and this life span extension requires the somatic gonad. Here we show that the somatic gonad and the germ cells make distinct contributions to life span determination. The life span increase produced by loss of the germ cells requires the DAF-16/FOXO transcription factor. In response to germ-cell removal, DAF-16 accumulates in nuclei. We find that the somatic gonad is not required for DAF-16 nuclear accumulation or for the increased stress resistance that is produced by germ-cell removal. The somatic gonad is required, however, for expression of specific DAF-16 target genes. DAF-16 is known to be activated by reduced insulin/IGF-1 signaling in C. elegans. In certain insulin/IGF-1-pathway mutants, the somatic gonad is not required for germ-cell removal to extend life span. Our genetic experiments suggest that these mutations reduce insulin/IGF-1 signaling below a critical threshold level. At these low levels of insulin/IGF-1 signaling, factors normally provided by the somatic gonad are no longer needed for germ-cell removal to increase the expression of DAF-16 target genes.</description><identifier>ISSN: 0016-6731</identifier><identifier>ISSN: 1943-2631</identifier><identifier>EISSN: 1943-2631</identifier><identifier>DOI: 10.1534/genetics.107.083253</identifier><identifier>PMID: 18202391</identifier><identifier>CODEN: GENTAE</identifier><language>eng</language><publisher>United States: Genetics Soc America</publisher><subject>Age ; Animals ; Caenorhabditis elegans - cytology ; Caenorhabditis elegans - genetics ; Caenorhabditis elegans - physiology ; Caenorhabditis elegans Proteins - metabolism ; Cell Nucleus - metabolism ; Cells ; Forkhead Transcription Factors ; Gene Expression Regulation, Developmental ; Germ Cells - metabolism ; Gonads - cytology ; Gonads - metabolism ; Insulin - metabolism ; Investigations ; Kinases ; Longevity - physiology ; Models, Biological ; Mutation ; Mutation - genetics ; Oxidative Stress ; Protein Transport ; Receptor, Insulin - metabolism ; Recombinant Fusion Proteins - metabolism ; Reproduction ; Rodents ; Signal Transduction ; Superoxide Dismutase - metabolism ; Transcription Factors - metabolism</subject><ispartof>Genetics (Austin), 2008-01, Vol.178 (1), p.513-526</ispartof><rights>Copyright Genetics Society of America Jan 2008</rights><rights>Copyright © 2008 by the Genetics Society of America</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c461t-81b0cf451d029418c953de5ca11287313662206cbaf1450634d2fda897ab79073</citedby><cites>FETCH-LOGICAL-c461t-81b0cf451d029418c953de5ca11287313662206cbaf1450634d2fda897ab79073</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18202391$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yamawaki, Tracy M</creatorcontrib><creatorcontrib>Arantes-Oliveira, Nuno</creatorcontrib><creatorcontrib>Berman, Jennifer R</creatorcontrib><creatorcontrib>Zhang, Peichuan</creatorcontrib><creatorcontrib>Kenyon, Cynthia</creatorcontrib><title>Distinct Activities of the Germline and Somatic Reproductive Tissues in the Regulation of Caenorhabditis elegans' Longevity</title><title>Genetics (Austin)</title><addtitle>Genetics</addtitle><description>The two parts of the Caenorhabditis elegans reproductive system, the germ cells and the somatic reproductive tissues, each influence the life span of the animal. Removing the germ cells increases longevity, and this life span extension requires the somatic gonad. Here we show that the somatic gonad and the germ cells make distinct contributions to life span determination. The life span increase produced by loss of the germ cells requires the DAF-16/FOXO transcription factor. In response to germ-cell removal, DAF-16 accumulates in nuclei. We find that the somatic gonad is not required for DAF-16 nuclear accumulation or for the increased stress resistance that is produced by germ-cell removal. The somatic gonad is required, however, for expression of specific DAF-16 target genes. DAF-16 is known to be activated by reduced insulin/IGF-1 signaling in C. elegans. In certain insulin/IGF-1-pathway mutants, the somatic gonad is not required for germ-cell removal to extend life span. Our genetic experiments suggest that these mutations reduce insulin/IGF-1 signaling below a critical threshold level. At these low levels of insulin/IGF-1 signaling, factors normally provided by the somatic gonad are no longer needed for germ-cell removal to increase the expression of DAF-16 target genes.</description><subject>Age</subject><subject>Animals</subject><subject>Caenorhabditis elegans - cytology</subject><subject>Caenorhabditis elegans - genetics</subject><subject>Caenorhabditis elegans - physiology</subject><subject>Caenorhabditis elegans Proteins - metabolism</subject><subject>Cell Nucleus - metabolism</subject><subject>Cells</subject><subject>Forkhead Transcription Factors</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Germ Cells - metabolism</subject><subject>Gonads - cytology</subject><subject>Gonads - metabolism</subject><subject>Insulin - metabolism</subject><subject>Investigations</subject><subject>Kinases</subject><subject>Longevity - physiology</subject><subject>Models, Biological</subject><subject>Mutation</subject><subject>Mutation - genetics</subject><subject>Oxidative Stress</subject><subject>Protein Transport</subject><subject>Receptor, Insulin - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Genetics (Austin)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yamawaki, Tracy M</au><au>Arantes-Oliveira, Nuno</au><au>Berman, Jennifer R</au><au>Zhang, Peichuan</au><au>Kenyon, Cynthia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Distinct Activities of the Germline and Somatic Reproductive Tissues in the Regulation of Caenorhabditis elegans' Longevity</atitle><jtitle>Genetics (Austin)</jtitle><addtitle>Genetics</addtitle><date>2008-01-01</date><risdate>2008</risdate><volume>178</volume><issue>1</issue><spage>513</spage><epage>526</epage><pages>513-526</pages><issn>0016-6731</issn><issn>1943-2631</issn><eissn>1943-2631</eissn><coden>GENTAE</coden><abstract>The two parts of the Caenorhabditis elegans reproductive system, the germ cells and the somatic reproductive tissues, each influence the life span of the animal. Removing the germ cells increases longevity, and this life span extension requires the somatic gonad. Here we show that the somatic gonad and the germ cells make distinct contributions to life span determination. The life span increase produced by loss of the germ cells requires the DAF-16/FOXO transcription factor. In response to germ-cell removal, DAF-16 accumulates in nuclei. We find that the somatic gonad is not required for DAF-16 nuclear accumulation or for the increased stress resistance that is produced by germ-cell removal. The somatic gonad is required, however, for expression of specific DAF-16 target genes. DAF-16 is known to be activated by reduced insulin/IGF-1 signaling in C. elegans. In certain insulin/IGF-1-pathway mutants, the somatic gonad is not required for germ-cell removal to extend life span. Our genetic experiments suggest that these mutations reduce insulin/IGF-1 signaling below a critical threshold level. At these low levels of insulin/IGF-1 signaling, factors normally provided by the somatic gonad are no longer needed for germ-cell removal to increase the expression of DAF-16 target genes.</abstract><cop>United States</cop><pub>Genetics Soc America</pub><pmid>18202391</pmid><doi>10.1534/genetics.107.083253</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Age Animals Caenorhabditis elegans - cytology Caenorhabditis elegans - genetics Caenorhabditis elegans - physiology Caenorhabditis elegans Proteins - metabolism Cell Nucleus - metabolism Cells Forkhead Transcription Factors Gene Expression Regulation, Developmental Germ Cells - metabolism Gonads - cytology Gonads - metabolism Insulin - metabolism Investigations Kinases Longevity - physiology Models, Biological Mutation Mutation - genetics Oxidative Stress Protein Transport Receptor, Insulin - metabolism Recombinant Fusion Proteins - metabolism Reproduction Rodents Signal Transduction Superoxide Dismutase - metabolism Transcription Factors - metabolism |
| title | Distinct Activities of the Germline and Somatic Reproductive Tissues in the Regulation of Caenorhabditis elegans' Longevity |
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