HIV-Particles in Spermatozoa of Patients with AIDS and Their Transfer into the Oocyte
By immunocytochemistry and in situ hybridization at the electron microscopy level, and by the PCR technique, we have shown that HIV-1 binds and enters normal sperm; that viral particles, their antigens, and nucleic acid are present in sperm from HIV-1 infected men; and that such sperm can transfer H...
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| Veröffentlicht in: | The Journal of cell biology 1994-11, Vol.127 (4), p.903-914 |
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| container_title | The Journal of cell biology |
| container_volume | 127 |
| creator | Baccetti, Baccio Benedetto, Arrigo Burrini, Anna G. Collodel, Giulia Ceccarini, Elvira Costantino Crisà, Nicla Di Caro, Antonino Estenoz, Mariela Garbuglia, Anna R. Massacesi, Adina Piomboni, Paola Renieri, Tommaso Solazzo, Delia |
| description | By immunocytochemistry and in situ hybridization at the electron microscopy level, and by the PCR technique, we have shown that HIV-1 binds and enters normal sperm; that viral particles, their antigens, and nucleic acid are present in sperm from HIV-1 infected men; and that such sperm can transfer HIV-1 like particles to normal human oocytes. We also present evidence that a galactosylceramide-like compound is present on the sperm membrane and could function as an alternative receptor for HIV. |
| doi_str_mv | 10.1083/jcb.127.4.903 |
| format | Article |
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that viral particles, their antigens, and nucleic acid are present in sperm from HIV-1 infected men; and that such sperm can transfer HIV-1 like particles to normal human oocytes. We also present evidence that a galactosylceramide-like compound is present on the sperm membrane and could function as an alternative receptor for HIV.</abstract><cop>United States</cop><pub>Rockefeller University Press</pub><pmid>7962075</pmid><doi>10.1083/jcb.127.4.903</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of cell biology, 1994-11, Vol.127 (4), p.903-914 |
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| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection; EZB Electronic Journals Library |
| subjects | Acquired immune deficiency syndrome Acquired Immunodeficiency Syndrome - pathology Acquired Immunodeficiency Syndrome - transmission Acquired Immunodeficiency Syndrome - virology AIDS AIDS/HIV Cell lines Cell Membrane - physiology Cell Membrane - ultrastructure Cellular biology Disease Transmission, Infectious DNA Female Fertilization Galactosylceramides - analysis HIV HIV 1 HIV-1 - isolation & purification HIV-1 - ultrastructure Human immunodeficiency virus Humans In Situ Hybridization Male Microscopy, Electron Oocytes - virology Polymerase Chain Reaction Receptors, HIV - analysis Reproduction RNA, Viral - analysis Spermatozoa Spermatozoa - pathology Spermatozoa - ultrastructure Spermatozoa - virology T lymphocytes Virions Viruses |
| title | HIV-Particles in Spermatozoa of Patients with AIDS and Their Transfer into the Oocyte |
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